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Photobiomodulation therapy upregulates the growth kinetics and multilineage differentiation potential of human dental pulp stem cells—an in vitro Study

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Abstract

This study aims to evaluate the impact of red LED irradiation on the viability, proliferation, colonogenic potential, markers expression along with osteogenic and chondrogenic differentiation of dental pulp stem cells. DPSCs were isolated from sound human permanent teeth using enzymatic digestion method and seeded with regular culture media. Cells at P4 were irradiated using red LED Light (627 nm, 2 J/cm2) and examined for growth kinetics, and multilineage differentiation using the appropriate differentiation media. The irradiated groups showed an increase in cellular growth rates, cell viability, clonogenic potential, and decrease in population doubling time compared to the control group. Cells of the irradiated groups showed enhanced differentiation towards osteogenic and chondrogenic lineages as revealed by histochemical staining using alizarin red and alcian blue stains. Photobiomodulation is an emerging promising element of tissue engineering triad besides stem cells, scaffolds, and growth factors.

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Data availability

The data supporting the findings of this study are available from the corresponding author upon request. Some data may not be made available because of privacy or ethical restrictions.

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Acknowledgements

We would like to express the deepest appreciation to Dr./Ahmed Mohamed Rakha, MSCs Endodontics, Faculty of Dentistry, Suez Canal University, Egypt for his great support and help throughout this research work. Special thanks to Prof. Dr. Ayman Abo-El Magd, Professor of physics, National Institute of Laser Enhanced Sciences, Cairo University, Egypt for help in the characterization of the LED device used in this study and to Prof. Dr. Abdel-Ghaffar Abo-El Soud, Professor of physiology, Faculty of Sciences, Suez Canal University, Egypt through all the steps of the research.

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Authors and Affiliations

  1. Oral Biology Department, Faculty of Dentistry, Suez Canal University, Ismailia, 41523, Egypt

    Lama E. Dawoud, Enas M. Hegazy & Mervat M. Youssef

  2. Human Anatomy and Embryology Department, Faculty of Medicine, Suez Canal University, Ismailia, 41523, Egypt

    Rania A. Galhom

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  1. Lama E. Dawoud
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Correspondence to Lama E. Dawoud.

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This study was performed in line with the principles of the Declaration of Helsinki. Approval was granted by the Ethics Committee of Faculty of Dentistry, Suez Canal University (approval number: 221/2019).

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Dawoud, L.E., Hegazy, E.M., Galhom, R.A. et al. Photobiomodulation therapy upregulates the growth kinetics and multilineage differentiation potential of human dental pulp stem cells—an in vitro Study. Lasers Med Sci 37, 1993–2003 (2022). https://doi.org/10.1007/s10103-021-03461-4

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