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Photobiomodulation induces hypotensive effect in spontaneously hypertensive rats

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Abstract

To evaluate whether acute photobiomodulation can elicit a hypotensive effect in spontaneously hypertensive rats (SHR). Male SHR were submitted to the implantation of a polyethylene cannula into the femoral artery. After 24 h, baseline measurements of the hemodynamic parameters: systolic, diastolic, and mean arterial pressure, and heart rate were accomplished for 1 h. Afterwards, laser application was simulated, and the hemodynamic parameters were recorded for 1 h. In the same animal, the laser was applied at six different positions of the rat’s abdomen, and the hemodynamic parameters were also recorded until the end of the hypotensive effect. The irradiation parameters were red wavelength (660 nm); average optical power of 100 mW; 56 s per point (six points); spot area of 0.0586 cm2; and irradiance of 1.71 W/cm2 yielding to a fluency of 96 J/cm2 per point. For measuring plasma NO levels, blood was collected before the recording, as well as immediately after the end of the mediated hypotensive effect. Photobiomodulation therapy was able to reduce the systolic arterial pressure in 69% of the SHR submitted to the application, displaying a decrease in systolic, diastolic, and mean arterial pressure. No change in heart rate was observed. Nevertheless, there was an increase in serum nitric oxide levels in the SHR responsive to photobiomodulation. Our results suggest that acute irradiation with a red laser at 660 nm can elicit a hypotensive effect in SHR, probably by a mechanism involving the release of NO, without changing the heart rate.

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Acknowledgments

We thank the DMC Equipment LTDA for providing the Photon Lase III.

Funding

This study was financed in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior—Brazil (CAPES)—(Finance Code 001) and by the São Paulo Research Foundation (FAPESP 2018/10588-9 and 2013/20549-7).

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Authors and Affiliations

  1. Departamento de Ciências Fisiológicas, Universidade Federal de São Carlos – UFSCar, Rod. Washington Luis, km 235, São Carlos, SP, CEP: 13565-905, Brazil

    Tereza C. Buzinari

  2. Department of Physiological Sciences, Federal University of São Carlos, São Carlos, SP, Brazil

    Tereza C. Buzinari, Thiago F. de Moraes & Gerson J. Rodrigues

  3. Department of Nursing, General and Specialized, School of Nursing of Ribeirão Preto, University of São Paulo – USP, Ribeirão Preto, SP, Brazil

    Evelin C. Cárnio

  4. Nucleus of Research and Teaching of Phototherapy in Health Sciences – NUPEN, São Carlos, SP, Brazil

    Luciana A. Lopes

  5. Department of Physiology, Ribeirão Preto Medical School, University of São Paulo – USP, Ribeirão Preto, SP, Brazil

    Helio C. Salgado

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  1. Tereza C. Buzinari
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  2. Thiago F. de Moraes
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Correspondence to Tereza C. Buzinari.

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All experimental protocols were performed in accordance with the guidelines of the Brazilian College for Animal Experimentation (COBEA), and were approved by the Ethical Committee for Animal of the Federal University of São Carlos—UFSCAR (no. 7936070618).

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Buzinari, T.C., de Moraes, T.F., Cárnio, E.C. et al. Photobiomodulation induces hypotensive effect in spontaneously hypertensive rats. Lasers Med Sci 35, 567–572 (2020). https://doi.org/10.1007/s10103-019-02849-7

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