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Microvascular imaging and monitoring of human oral cavity lesions in vivo by swept-source OCT-based angiography

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Abstract

We report the development of optical coherence tomography- (OCT) based angiography (OCTA) to image blood flow within microcirculatory tissue beds in human oral cavity in vivo with a field of view at 10 mm × 10 mm. Three-dimensional (3D) structural and vascular images of labial mucosa tissue are obtained at a single 3D acquisition. Pathologic mucosal sites with mouth ulcers are examined using the OCT tomograms and angiograms, upon which to monitor the lesion healing process over a period of 2 weeks. Quantitative metrics of the capillary loop density within the lamina propria layer are evaluated, providing statistically significant difference between healthy and diseased conditions over time. Furthermore, tissue anatomy and vessel morphology of other susceptible sites to ulcer, such as tongue, alveolar mucosa, and labial frenulum, are also imaged to demonstrate the promise of the proposed method as a clinically useful tool for the diagnosis and monitoring of therapeutic treatment of oral tissue abnormalities.

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Funding

This work was supported in part by research grants from the National Heart, Lung, and Blood Institute (R01 HL093140). The content is solely the responsibility of the authors and does not necessarily represent the official views of grant giving bodies.

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Author notes

  1. Wei Wei and Woo June Choi contributed equally to this work.

Authors and Affiliations

  1. Department of Bioengineering, University of Washington, 3720 15th Ave NE, Seattle, WA, 98195, USA

    Wei Wei, Woo June Choi & Ruikang K. Wang

Authors
  1. Wei Wei
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  2. Woo June Choi
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  3. Ruikang K. Wang
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Correspondence to Ruikang K. Wang.

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The authors declare that they have no conflict of interest.

Ethical approval

The in vivo imaging study that uses home-built systems to image human subjects was reviewed and approved by the Institutional Review Board of University of Washington, and the informed written consent was obtained from all subjects before imaging. This study followed the tenets of the Declaration of Helsinki and was conducted in compliance with the Health Insurance Portability and Accountability Act.

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Wei, W., Choi, W.J. & Wang, R.K. Microvascular imaging and monitoring of human oral cavity lesions in vivo by swept-source OCT-based angiography. Lasers Med Sci 33, 123–134 (2018). https://doi.org/10.1007/s10103-017-2350-3

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  • DOI: https://doi.org/10.1007/s10103-017-2350-3

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