Abstract
The aim of this study was to evaluate the performance of a pen-type laser fluorescence (LF) device (LFpen: DIAGNOdent pen) to detect and monitor the progression of caries-like lesions on smooth surfaces. Fifty-two bovine enamel blocks were submitted to three different demineralisation cycles for caries-like lesion induction using Streptococcus mutans, Lactobacillus casei and Actinomyces naeslundii. At baseline and after each cycle, the enamel blocks were analysed under Knoop surface micro-hardness (SMH) and an LFpen. One enamel block after each cycle was randomly chosen for Raman spectroscopy analysis. Cross-sectional micro-hardness (CSMH) was performed at different depths (20, 40, 60, 80 and 100 μm) in 26 enamel blocks after the second cycle and 26 enamel blocks after the third cycle. Average values of SMH (± standard deviation (SD)) were 319.3 (± 21.5), 80.5 (± 31.9), 39.8 (± 12.7), and 29.77 (± 10.34) at baseline and after the first, second and third cycles, respectively. Statistical significant difference was found among all periods (p < 0.01). The LFpen values were 4.3 (± 1.5), 7.5 (± 9.4), 7.1 (± 7.1) and 5.10 (± 3.58) at baseline and after the first, second, and third cycles, respectively, among all periods (p < 0.05). The CSMH values after the second and third cycles at 20, 40, 60, 80 and 100 μm were 182.8 (± 69.8), 226.1 (± 79.6), 247.20 (± 69.36), 262.35 (± 66.36) and 268.45 (± 65.49), and for the third cycle were 193.7 (± 73.4), 239.5 (± 81.5), 262.64 (± 82.46), 287.10 (± 78.44) and 284.79 (± 72.63) (n = 24 and 23), respectively. No correlation was observed between the LFpen and SMH values (p > 0.05). One sample of each cycle was characterised through Raman spectroscopy analysis. It can be concluded that LF was effective in detecting the first demineralisation on enamel; however, the method did not show any effect in monitoring lesion progression after three cycles of in vitro demineralisation.
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Pretty IA, Maupome G (2004) A closer look at diagnosis in clinical dental practice: part 5. Emerging technologies for caries detection and diagnosis. J can Dent Assoc 70:540–540a–540i
Gimenez T, Bispo BA, Souza DP et al (2016) Does the decline in caries prevalence of Latin American and Caribbean children continue in the new century? Evidence from systematic review with meta-analysis. PLoS One 11:e0164903. doi:10.1371/journal.pone.0164903
Tagliaferro EPS, Meneghim MC, Ambrosano GMB et al (2008) Distribution and prevalence of dental caries in Bauru, Brazil, 1976–2006. Int Dent J 58:75–80
Bader JD, Shugars DA (2006) The evidence supporting alternative management strategies for early occlusal caries and suspected occlusal dentinal caries. J Evid Based Dent Pract 6:91–100. doi:10.1016/j.jebdp.2005.12.004
Spiguel MH, Tovo MF, Kramer PF et al (2009) Evaluation of laser fluorescence in the monitoring of the initial stage of the de-/remineralization process: an in vitro and in situ study. Caries Res 43:302–307. doi:10.1159/000218094
Lussi A, Francescut P (2003) Performance of conventional and new methods for the detection of occlusal caries in deciduous teeth. Caries Res 37:2–7
Moriyama CM, Rodrigues JA, Lussi A, Diniz MB (2014) Effectiveness of fluorescence-based methods to detect in situ demineralization and remineralization on smooth surfaces. Caries Res 48:507–514. doi:10.1159/000363074
Rodrigues JA, Hug I, Diniz MB, Lussi A (2008) Performance of fluorescence methods, radiographic examination and ICDAS II on occlusal surfaces in vitro. Caries Res 42:297–304. doi:10.1159/000148162
Braun A, Krause F, Jepsen S (2005) The influence of the calibration mode of a laser fluorescence device on caries detection. Caries Res 39:144–149. doi:10.1159/000083161
Aljehani A, Yang L, Shi X-Q (2007) In vitro quantification of smooth surface caries with DIAGNOdent and the DIAGNOdent pen. Acta Odontol Scand 65:60–63. doi:10.1080/00016350601058051
Mendes FM, Hissadomi M, Imparato JCP (2004) Effects of drying time and the presence of plaque on the in vitro performance of laser fluorescence in occlusal caries of primary teeth. Caries Res 38:104–108. doi:10.1159/000075933
Mendes FM, Siqueira WL, Mazzitelli JF et al (2005) Performance of DIAGNOdent for detection and quantification of smooth-surface caries in primary teeth. J Dent 33:79–84. doi:10.1016/j.jdent.2004.10.010
Paes Leme AF, Tabchoury CPM, Zero DT, Cury JA (2003) Effect of fluoridated dentifrice and acidulated phosphate fluoride application on early artificial carious lesions. Am J Dent 16:91–95
Pinelli C, Campos Serra M, de Castro Monteiro Loffredo L (2002) Validity and reproducibility of a laser fluorescence system for detecting the activity of white-spot lesions on free smooth surfaces in vivo. Caries Res 36:19–24
Shi XQ, Tranaeus S, Angmar-Mansson B (2001) Validation of DIAGNOdent for quantification of smooth-surface caries: an in vitro study. Acta Odontol Scand 59:74–78
Yassen GH, Platt JA, Hara AT (2011) Bovine teeth as substitute for human teeth in dental research: a review of literature. J Oral Sci 53:273–282
Arthur RA, Martins VB, de Oliveira CL et al (2015) Effect of over-the-counter fluoridated products regimens on root caries inhibition. Arch Oral Biol 60:1588–1594. doi:10.1016/j.archoralbio.2015.07.018
Cury JA, Rebelo MA, Del Bel Cury AA et al (2000) Biochemical composition and cariogenicity of dental plaque formed in the presence of sucrose or glucose and fructose. Caries Res 34:491–497
Lussi A, Hellwig E (2006) Performance of a new laser fluorescence device for the detection of occlusal caries in vitro. J Dent 34:467–471. doi:10.1016/j.jdent.2005.11.002
Lussi A, Imwinkelried S, Pitts N et al (1999) Performance and reproducibility of a laser fluorescence system for detection of occlusal caries in vitro. Caries Res 33:261–266
De Campos PH, Sanabe ME, Rodrigues JA et al (2015) Different bacterial models for in vitro induction of non-cavitated enamel caries-like lesions: microhardness and polarized light miscroscopy analyses. Microsc Res Tech 78:444–451. doi:10.1002/jemt.22493
Purdell-Lewis DJ, Groeneveld A, Arends J (1976) Hardness tests on sound enamel and artificially demineralized white spot lesions. Caries Res 10:201–215
Arends J, Schuthof J, Jongebloed WG (1979) Microhardness indentations on artificial white spot lesions. Caries Res 13:290–297
Diniz MB, Paes Leme AF, Cardoso K d S et al (2009) The efficacy of laser fluorescence to detect in vitro demineralization and remineralization of smooth enamel surfaces. Photomed Laser Surg 27:57–61. doi:10.1089/pho.2007.2230
Gokalp S, Baseren M (2005) Use of laser fluorescence in monitoring the durability and cariostatic effects of fluoride and chlorhexidine varnishes on occlusal caries: a clinical study. Quintessence Int 36:183–189
Mohanty B, Dadlani D, Mahoney D, Mann AB (2013) Characterizing and identifying incipient carious lesions in dental enamel using micro-Raman spectroscopy. Caries Res 47:27–33. doi:10.1159/000342432
Acknowledgements
We acknowledge the Post-Graduate Programme in Dentistry of the Federal University of Paraná for the use of their laboratory for micro-hardness analysis and FAPERGS (Fundação de Amparo a Pesquisa do Estado do Rio Grande do Sul) for the financial support (grant ARD no. 11/1737-9). The funder had no role in study the design, data collection and analysis, decision to publish or preparation of the manuscript.
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This study was approved by the Research and Ethics Committee on Animal Use of the Federal University of Rio Grande do Sul (process no. 20576) since bovine teeth were used. Therefore, no informed consent was necessary.
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Rodrigues, J.A., Sarti, C.S., Assunção, C.M. et al. Evaluation of laser fluorescence in monitoring non-cavitated caries lesion progression on smooth surfaces in vitro. Lasers Med Sci 32, 1793–1800 (2017). https://doi.org/10.1007/s10103-017-2262-2
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DOI: https://doi.org/10.1007/s10103-017-2262-2