Skip to main content

Advertisement

SpringerLink
Log in

A study of diagnostic criteria established for two oral mucous diseases by HMME-fluorescence spectroscopy

  • Original Article
  • Published:
Lasers in Medical Science Aims and scope Submit manuscript

Abstract

Malignant oral ulcers are common pathological occurrence in oral and maxillofacial tumors. A noninvasive method for diagnosis of malignant oral ulcers was developed in the study, which is based on hematoporphyrin monomethylether (HMME) fluorescence spectroscopy. The objective of this work is to determine the feasibility of this method in differentiating the malignant tissues from the inflammatory ones in the hamster cheek pouch model. Adult hamsters were used for the study and a cheek pouch model was established. For the malignant model, the 9, 10-dimethyl-1, 2-benzanthracene carcinogenesis was applied to one cheek pouch for 10 weeks (N = 35). The simple ulcers were created on buccal cheek mucosa in a simple manner (N = 10). Prior to sacrifice, HMME solution was injected into the tissues. The induced fluorescence spectra of the cheek tissues were recorded by a fiber spectrometer with excitation at 405 nm. A spectral algorithm was used to eliminate the effect of autofluorescence, and a spectral parameter S was selected as diagnostic criterion. After fluorescence measurement, the animals were sacrificed and the measured tissues were collected. Histological staining was performed and the results of histopathological evaluation were documented. The diagnostic criteria that reflected the fluorescence intensity were set as follows: normal, S ≤ 10; simple ulcer, 230 ≤ S ≤ 290; and malignant ulcer, 140 ≤ S ≤ 200. The sensitivity and specificity of this detection method was verified by scalpel biopsy, and the overall accuracy was over 90 %. The results of this study showed that the fluorescence spectroscopic method implemented by HMME can accurately differentiate the two kinds of clinically indistinguishable diseases.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  1. Compilato D, Cirillo N, Termine N et al (2009) Long-standing oral ulcers: proposal for a new ‘S-C-D classification system’. J Oral Pathol Med 38:241–253

    Article  CAS  PubMed  Google Scholar 

  2. Güneri P, Epstein JB, Kaua A et al (2011) The utility of toluidine blue staining and brush cytology as adjuncts in clinical examination of suspicious oral mucosal lesions. Int J Oral Maxillofac Surg 40:155–161

    Article  PubMed  Google Scholar 

  3. Scully C (2008) Soreness and ulcers. In: Oral and maxillofacial medicine. Wright Publishing Co., Edinburgh, p 171–181

  4. Zuluage AF, Utzinger URS, Durkin A et al (1999) Fluorescence excitation emission matrices of human tissue: a system for in vivo measurement and method of data analysis. Appl Spectrosc 53:302–311

    Article  Google Scholar 

  5. Heintzelman DL, Utzinger U, Fuchs H et al (2000) Optimal excitation wavelengths for in vivo detection of oral neoplasia using fluorescence spectroscopy. Photochem Photobiol 72:103–113

    Article  CAS  PubMed  Google Scholar 

  6. Rajaram N, Reichenberg JS, Migden MR et al (2010) Pilot clinical study for quantitave spectral diagnosis of non-melanoma skin cancer. Lasers Surg Med 42:716–727

    PubMed Central  PubMed  Google Scholar 

  7. Lingen MW, Kalmar JR, Karrison T et al (2008) Critical evaluation of diagnostic aids for the detection of oral cancer. Oral Oncol 44:10–22

    Article  PubMed Central  PubMed  Google Scholar 

  8. Kennedy JC, Pottier RH (1992) Endogenous protoporphyrin IX, a clinically useful photosensitizer for photodynamic therapy. J Photochem Photobiol B 14:275–292

    Article  CAS  PubMed  Google Scholar 

  9. Ebihara A, Krasieva TB, Liaw L-HL et al (2003) Detection and diagnosis of oral cancer by light-induced fluorescence. Lasers Surg Med 32:17–24

    Article  PubMed  Google Scholar 

  10. Fan KFM, Hopper C, Speight PM et al (1996) Photodynamic therapy using 5-Aminolevulinic acid for premalignant and malignant lesions of the oral cavity. Cancer 78:1374–1383

    Article  CAS  PubMed  Google Scholar 

  11. Van der Neen N, de Bruijn HS, Berg RWJ et al (1996) Kinetics and localization of PpIX fluorescence after topical and systemic ALA application, observed in skin and skin tumours of UVB treated mice. Br J Cancer 73:925–930

    Article  Google Scholar 

  12. Zhang L, Bi LJ, Shi JN, Zhang ZG et al (2013) A quantitative diagnostic method for oral mucous precancerosis by Rose Bengal fluorescence spectroscopy. Lasers Med Sci 28:241–246

    Article  PubMed  Google Scholar 

  13. Sun Y, Xing DF, Shen LH et al (2013) Bactericidal effects of hematoporphyrin monomethylether-mediated photosensitization against pathogenic communities from supragingival plaque. Appl Microbiol Biotechnol 97:5079–5087

    Article  CAS  PubMed  Google Scholar 

  14. Shi JJ, Ma RR, Wang L et al (2013) The application of hyaluronic acid-derivatized carbon nanotubes in hematoporphyrin monomethyl ether-based photodynamic therapy for in vivo and in vitro cancer treatment. Int J Nanomedicine 8:2361–2373

    Article  PubMed Central  PubMed  Google Scholar 

  15. Lei TC, Glazner GF, Duffy M et al (2012) Optical properties of hematoporphyrin monomethyl ether (HMME), a PDT photosensitizer. Photodiagn Photodyn Ther 9:232–242

    Article  CAS  Google Scholar 

  16. MacDonald DG (1981) Comparison of epithelial dysplasia in hamster cheek pouch carcinogenesis and human oral mucosa. J Oral Pathol 10:186–191

    Article  CAS  PubMed  Google Scholar 

  17. Lu J, Pei H, Kaeck M (1997) Gene expression changes associated with chemically induced rat mammary carcinogenesis. Mol Carcinog 20:204–215

    Article  CAS  PubMed  Google Scholar 

  18. Bedard N, Pierce M, El-Nagger A (2010) Emerging roles for multimodal optical imaging in early cancer detection: a global challenge. Technol Cancer Res Treat 9:211–217

    Article  PubMed Central  PubMed  Google Scholar 

  19. Roblyer D, Kurachi C, Stepanek V et al (2009) Objective detection and delineation of oral neoplasia using autofluorescence imaging. Cancer Prev Res (Phila) 2:423–431

    Article  Google Scholar 

  20. Qin YL, Luan XL, Bi LJ et al (2007) Real-time detection of dental calculus by blue-LED-induced fluorescence spectroscopy. J Photochem Photobiol B 87:88–94

    Article  CAS  PubMed  Google Scholar 

  21. Jiang CF, Wang CY, Chiang CP (2011) Comparative study of protoporphyrin IX fluorescence image enhancement methods to improve an optical imaging system for oral cancer detection. J Biomed Opt 16:076006

    Article  PubMed  Google Scholar 

  22. Souza LR, Fonseca-Silva T, Pereira CS et al (2011) Immunohistochemical analysis of p53, APE1, hMSH2 and ERCC1 proteins in actinic cheilitis and lip squamous cell carcinoma. Histopathology 58:352–360

    Article  PubMed  Google Scholar 

Download references

Acknowledgments

This research was supported by Hei Long Jiang Postdoctoral Foundation (No. LBH-Z12208), and the First Affiliated Hospital of Harbin Medical University Foundation (No. 2014B19).

Author information

Authors and Affiliations

  1. Department of Oral and Maxillofacial Surgery, The First Affiliated Hospital, Harbin Medical University, Harbin, 150001, People’s Republic of China

    Moyang Lv, Limin Mao & Shaohua Lv

  2. Condensed Matter Science and Technology Institute, Harbin Institute of Technology, Harbin, 150080, People’s Republic of China

    Feng Qin & Zhiguo Zhang

  3. Department of Preventive Dentistry, The First Affiliated Hospital, Harbin Medical University, Harbin, 150001, People’s Republic of China

    Lei Zhang

  4. BoRui Dental Clinic, Harbin, 150001, People’s Republic of China

    Jian Jin

Authors
  1. Moyang Lv
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Feng Qin
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Limin Mao
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Lei Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Shaohua Lv
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Jian Jin
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Zhiguo Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Limin Mao or Lei Zhang.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Lv, M., Qin, F., Mao, L. et al. A study of diagnostic criteria established for two oral mucous diseases by HMME-fluorescence spectroscopy. Lasers Med Sci 30, 2151–2156 (2015). https://doi.org/10.1007/s10103-015-1776-8

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10103-015-1776-8

Keywords

Search

Navigation