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Inactivation of Aggregatibacter actinomycetemcomitans by two different modalities of photodynamic therapy using Toluidine blue O or Radachlorin as photosensitizers: an in vitro study

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Abstract

Aggregatibacter actinomycetemcomitans (A. actinomycetemcomitans) is one of the periodontopathogens strongly associated with aggressive periodontitis. The aim of this investigation was to compare the effect of laser and light-emitting diode on the photodynamic inactivation of A. actinomycetemcomitans. Eighty-four samples of bacterial suspensions (200 μL) were prepared and divided in seven groups: control group (no treatment), laser group (indium-gallium-aluminum-phosphate laser with wavelength of 662 ± 0.1 nm, energy density of 6 j/cm2, and irradiation time of 34 s), light-emitting diode (LED) group (wavelength 625–635 nm, energy density 6 j/cm2, time of irradiation 30 s), Toluidine blue O (TBO) group (0.1 mg/mL), Radachlorin group (0.1 %), Radachlorin + laser group (after pre-irradiation time of 10 min, laser was irradiated), and TBO + LED group (after preirradiation time of 10 min, LED was irradiated). Then, 100 μL of each sample was cultured in brain heart infusion (BHI) plates and incubated for 48–72 h in microaerophilic atmosphere for colony counting. Application of Radachlorin + laser resulted in a significant decrease in the concentration of A. actinomycetemcomitans (P values <0.05). Photodynamic therapy with laser + Radachlorin was more effective than that of LED + TBO in suppression of this microorganism (P value <0.05). Within the limits of this study, it can be concluded that photodynamic inactivation using laser and Radachlorin was more effective than that of LED and TBO in eradication of A. actinomycetemcomitans.

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Acknowledgments

This research was supported by a grant from the Laser Research Center of Dentistry, Tehran University of Medical Sciences (number 89-04-97-11854). The authors would like to thank Dr. Raika Jamali, assistant professor of Tehran University of Medical Sciences for his special help in performing statistical analyses.

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Authors and Affiliations

  1. Laser Research Center of Dentistry, Tehran University of Medical Sciences, Tehran, Iran

    Neda Moslemi, Pardis Soleiman-zadeh Azar, Nasim Chiniforush & Reza Fekrazad

  2. Department of Periodontics, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran

    Neda Moslemi, Nina Rouzmeh & Mojgan Paknejad

  3. Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran

    Abbas Bahador

  4. Dental Research Center, Tehran University of Medical Sciences, Tehran, Iran

    Mojgan Paknejad

  5. Laser Research Center in Medical Sciences, AJA University of Medical Sciences, Tehran, Iran

    Reza Fekrazad

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  1. Neda Moslemi
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  2. Pardis Soleiman-zadeh Azar
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Correspondence to Mojgan Paknejad.

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Moslemi, N., Soleiman-zadeh Azar, P., Bahador, A. et al. Inactivation of Aggregatibacter actinomycetemcomitans by two different modalities of photodynamic therapy using Toluidine blue O or Radachlorin as photosensitizers: an in vitro study. Lasers Med Sci 30, 89–94 (2015). https://doi.org/10.1007/s10103-014-1621-5

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  • DOI: https://doi.org/10.1007/s10103-014-1621-5

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