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Raman spectroscopy detection of molecular changes associated with two experimental models of osteoarthritis in rats

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Abstract

The aim of the present study was to evaluate the feasibility of applying Raman spectroscopy in probing the molecular changes in terms of collagen deposition and tissue remodeling associated with two well-established experimental models of osteoarthritis (OA) in knee of rats. In order to evaluate alterations in the articular surface area, the menisci-covered tibial region was assessed into three groups as follows: control (joint preserved) and two models of experimental knee OA: collagenase-induced model (n = 8) and treadmill exercise-induced model (n = 8). Each group was examined for molecular changes using spectral parameters related to cartilage, subchondral bone, and bone tissues. A significant increase of Raman ratios related to mineralization and tissue remodeling was found (p < 0.05), suggesting that both models were successful for inducing OA in rats. The significantly lower phenylalanine content and higher crystallinity in the treadmill exercise-induced model of OA than collagenase-induced model of OA (p < 0.05) indicated that the OA pathogenesis was model-dependent. Thus, this work suggests that the Raman spectroscopy technique has potential for the diagnosis and detection of cartilage damage and monitoring of subchondral bone and bone in OA pathogenesis at the molecular level.

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Acknowledgments

R.A. Souza thanks FAPEMIG (Fundação de Amparo a Pesquisa do Estado de Minas Gerais) for the research grant support (APQ-02900-10). M. Xavier thanks FAPEMIG for the research grant support (APQ-01733-11). L. Silveira Jr. thanks FAPESP (São Paulo Research Foundation) and CNPq (National Counsel of Technological and Scientific Development) for the partial financial support (2009/01788-5 and 483026/2010-7, respectively).

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Authors and Affiliations

  1. Grupo de Estudos e Pesquisa em Ciências da Saúde (GEP-CS), Instituto Federal de Educação, Ciência e Tecnologia do Sul de Minas Gerais, Campus Muzambinho. Estrada de Muzambinho, km 35, Caixa Postal 02, 37890-000, Muzambinho, Minas Gerais, Brazil

    Renato Aparecido de Souza

  2. Department of Physiotherapy, Federal University of Jequitinhonha and Mucuri Valleys, UFVJM. Rodovia MGT 367 - Km 583, no. 5000, 39100-000, Diamantina, Minas Gerais, Brazil

    Murilo Xavier & Ana Paula Santos

  3. Department of Physiotherapy, Federal University of Piauí, UFPI, Campus Parnaíba, Av. São Sebastião Reis Velloso, 2819, 64202-020, Parnaiba, Piaui, Brazil

    Nilton Maciel Mangueira

  4. Center of Biophotonics, School of Dentistry, Federal University of Bahia, UFBA, Av. Araújo Pinho, 62–Canela, 40110-150, Salvador, Bahia, Brazil

    Antonio Luiz Barbosa Pinheiro

  5. Biomedical Engineering Institute, Universidade Camilo Castelo Branco—UNICASTELO, Parque Tecnológico de São José dos Campos, Estrada Dr. Altino Bondesan, 500, São José dos Campos, Sao Paulo, Brazil

    Antonio Luiz Barbosa Pinheiro, Antonio Balbin Villaverde & Landulfo Silveira Jr.

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  1. Renato Aparecido de Souza
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  2. Murilo Xavier
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  3. Nilton Maciel Mangueira
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  4. Ana Paula Santos
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  7. Landulfo Silveira Jr.
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Correspondence to Renato Aparecido de Souza.

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de Souza, R.A., Xavier, M., Mangueira, N.M. et al. Raman spectroscopy detection of molecular changes associated with two experimental models of osteoarthritis in rats. Lasers Med Sci 29, 797–804 (2014). https://doi.org/10.1007/s10103-013-1423-1

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  • DOI: https://doi.org/10.1007/s10103-013-1423-1

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