Abstract
Analysis of bacterial DNA using a polymerase chain reaction performed with broad-range eubacterial 16S rDNA primers may yield a diagnosis of bacterial meningitis in cases where Gram staining of cerebrospinal fluid (CFS), antigen detection techniques or culture fail. Since this PCR technique occasionally gives false-positive results due to contamination of samples or laboratory reagents, a study was performed to establish the diagnostic value of assaying concentrations of tumour necrosis factor-α (TNF-α) and interleukin-10 (IL-10) in 90 CSF samples. A high correlation was found between a positive PCR result and the concentrations of TNF-α and IL-10, indicating that cytokine assays may be used as a confirmatory test. The findings suggested that a combination of the PCR technique, amplicon sequencing and assay of TNF-α and IL-10 concentrations in CSF is a reliable and cost-effective procedure for diagnosis of culture-negative bacterial meningitis.
Similar content being viewed by others
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Lorino, G., Angeletti, S., Gherardi, G. et al. Diagnostic Value of Cytokine Assays in Cerebrospinal Fluid in Culture-Negative, Polymerase Chain Reaction-Positive Bacterial Meningitis. EJCMID 19, 388–392 (2000). https://doi.org/10.1007/s100960050501
Issue Date:
DOI: https://doi.org/10.1007/s100960050501