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Statistical optimization of environmental factors to produce the cytotoxic enniatins H, I and MK1688 against human multidrug resistance cancer cell lines

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Abstract

The environmental conditions were optimized to produce the enniatin H, I, and MK1688 by Fusarium strain on cereal grain exhibiting anti-carcinogenic potential against MES-SA (human uterine sarcoma cell line), HCT15 (human colorectal carcinoma cancer cell line), and their multidrug resistance sublines. From the statistical optimization by response surface methodology, the optimal condition of independent variables affecting the response variables were 20.85 °C (temperature), 46.85% (w/w, initial moisture content), and 18.42 days (growth time) for ENN H; 23.31 °C, 44.15% (w/w) and 17.23 days for ENN I; 23.08 °C, 43.97% (w/w) and 17.06 days for ENN MK1688. In case of cytotoxic effects, ENNs significantly suppressed growth of cancer cell lines without multidrug resistance, and ENN I inhibited growth of cancer cell lines most strongly. These data will provide valuable point to produce the cyclic hexadepsipeptide exhibiting anti-carcinogenic potential from Fusarium strains.

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Acknowledgements

This research was supported by his research was supported by a grant (21153MFDS605) from the Ministry of Food and Drug Safety, and the Chung-Ang University Graduate Research Scholarship (academic scholarship for College of Biotechnology and Natural Resources) in 2021.

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Authors and Affiliations

  1. Department of Food Safety and Regulatory Science, Chung-Ang University, Anseong, 17546, Republic of Korea

    YoungMin Hwang & Hee-Seok Lee

  2. Department of Food Science and Biotechnology, Chung-Ang University, Anseong, 17546, Republic of Korea

    Hee-Seok Lee

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  1. YoungMin Hwang
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Correspondence to Hee-Seok Lee.

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Hwang, Y., Lee, HS. Statistical optimization of environmental factors to produce the cytotoxic enniatins H, I and MK1688 against human multidrug resistance cancer cell lines. Food Sci Biotechnol 33, 579–587 (2024). https://doi.org/10.1007/s10068-023-01363-5

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