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Chemical profiling and antioxidants screening from natural products: using CiNingJi as an example

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Abstract

Natural products with good antioxidative properties have been paid increased attention globally. However, due to its chemical complexity, it is difficult to find out its antioxidative compounds. Herein, the chemical profiling and antioxidant capacity of CiNingJi (CNJ) were analyzed, as an example. By using UHPLC-Q-TOF/MS, a total of 82 compounds were tentatively deduced. Furthermore, its free radical scavenging capacity was assessed by different in vitro spectrophotometric-based assays. The result showed that one ingredient, Rosa roxburghii, plays a critical role in its antioxidant activity. In addition, 18 potential antioxidants were screened out in CNJ by comparing the difference of it with and without DPPH reaction. They were identified mainly as catechin, ellagic acid, kajiichigoside F1, and their derivatives or isomers. With the further quantification of major found antioxidants, our results may provide some knowledge on predicting the antioxidative compounds of natural products.

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Acknowledgements

This study was funded by the Guangzhou Institute of Respiratory Health Open Project (Funds provided by China Evergrande Group) (Project No.:2020GIRHHMS04).

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Author notes

  1. Yida Zhang & Peiyan Zheng

    Present address: Department of Allergy and Clinical Immunology, National Center for Respiratory Medicine, National Clinical Research Center for Respiratory Disease, State Key Laboratory of Respiratory Disease, Guangzhou Institute of Respiratory Health, The First Affiliated Hospital of Guangzhou Medical University, No.151 Yanjiang Xi Lu, Yuexiu District, Guangzhou, China

  2. Yida Zhang, Peiyan Zheng and Guanyu Yan are co-first authors and contributed equally to this work.

Authors and Affiliations

  1. Department of Allergy and Clinical Immunology, National Center for Respiratory Medicine, National Clinical Research Center for Respiratory Disease, State Key Laboratory of Respiratory Disease, Guangzhou Institute of Respiratory Health, The First Affiliated Hospital of Guangzhou Medical University, No.151 Yanjiang Xi Lu, Yuexiu District, Guangzhou, China

    Guanyu Yan & Sun Baoqing

  2. State Key Laboratory of Quality Research in Chinese Medicine, Macau Institute for Applied Research in Medicine and Health, Macau University of Science and Technology, Macao, China

    Wu Jian-lin

  3. Science and Technology Innovation Center, Guangzhou University of Chinese Medicine, 510405, Guangzhou, China

    Zhuo Yue

Authors
  1. Yida Zhang
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Contributions

Yida Zhang, Peiyan Zheng and Guanyu Yan contributed equally to this work. Yida Zhang analyzed the data, interpreted the results, and drafted the manuscript. Guanyu Yan performed the experiments. Yue Zhuo provided guidance for experiments. Peiyan Zheng critically revised the manuscript. Jian-lin Wu and Baoqing Sun conceived and designed the study. All authors read and approved the final manuscript.

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Correspondence to Sun Baoqing.

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Zhang, Y., Zheng, P., Yan, G. et al. Chemical profiling and antioxidants screening from natural products: using CiNingJi as an example. Food Sci Biotechnol 31, 407–421 (2022). https://doi.org/10.1007/s10068-022-01049-4

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  • DOI: https://doi.org/10.1007/s10068-022-01049-4

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