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β-Glucan from Aureobasidum species inhibits fat accumulation in 3T3-L1 adipocyte differentiation

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Abstract

The anti-fat accumulative effects of bacteria-derived β-glucan in 3T3-L1 adipocyte differentiation was investigated. 3T3-L1 preadipocytes were treated with β-glucan at 50 and 100 μg/mL during adipogenesis. Oil Red O staining was used to study expressions of the adipogenic genes PPARγ, ACC, and aP2. Adipogenesis of 3T3-L1 cells treated with β-glucan at 50 and 100 μg/mL was significantly (p<0.05) inhibited by 32.8 and 39.7%, respectively, compared with controls. Expressions of the PPARγ gene from 3T3-L1 cells treated with β-glucan at 50 and 100 μg/mL were significantly (p<0.05) decreased by 13.6 and 46.1%, respectively, ACC gene expression by 18.3 and 36.0%, respectively, and aP2 gene expression by 36.8 and 56.7%, respectively, compared to adipocyte controls. Bacteria-derived β-glucan inhibits fat accumulation at the cellular level that is mediated via decreases in expressions of the PPARγ, ACC, and aP2 genes.

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Authors and Affiliations

  1. College of Biotechnology and Bioengineering, Sungkyunkwan University, Suwon, Gyeonggi, 440-746, Korea

    Mi-Ja Kim

  2. Department of Fermented Food Science, Seoul Venture University, Seoul, 137-070, Korea

    Ork-Hee Kim & Chul Cheong

  3. Department of Food & Nutrition, Kangwon National University, Samcheok, Gangwon, 245-711, Korea

    Ki-Hyo Jang

  4. Biotechnology Research Division, Korea Research Institute of Bioscience and Biotechnology, Daejeon, 306-809, Korea

    Chul-Ho Kim

  5. Department of Conversing Technology, Graduate School of Venture, Institute of Health Industry, Hoseo University, Seoul, 137-867, Korea

    Soon Ah Kang

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  1. Mi-Ja Kim
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  2. Ork-Hee Kim
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  3. Chul Cheong
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  4. Ki-Hyo Jang
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Correspondence to Soon Ah Kang.

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Kim, MJ., Kim, OH., Cheong, C. et al. β-Glucan from Aureobasidum species inhibits fat accumulation in 3T3-L1 adipocyte differentiation. Food Sci Biotechnol 24, 1147–1150 (2015). https://doi.org/10.1007/s10068-015-0146-4

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  • DOI: https://doi.org/10.1007/s10068-015-0146-4

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