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Attachment and biofilm formation of Pseudomonas fluorescens PSD4 isolated from a dairy processing line

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Abstract

The effects of different nutrient sources and temperatures on attachment and biofilm formation of Pseudomonas fluorescens PSD4, a dairy isolate, were studied. Initial adherence and attachment capabilities among different strains were studied using microtitre plate assays. Biofilm development was observed using confocal microscopy. Strongly adherent cells were seen in protein rich media. Citrate as a carbon source enhanced biofilm formation. Glucose did not favor biofilm development. Psychrotrophic P. fluorescens PSD4 formed strongly adherent biofilms having high metabolic activities at low temperatures. P. fluorescens PSD4 with spoilage potential was capable of forming strong biofilms in dairy processing environments. Biofilm formation was influenced by nutrient availability and growth conditions. These factors should be considered for design of effective anti-biofilm strategies.

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Authors and Affiliations

  1. Department of Studies in Microbiology, University of Mysore, Mysore, 570 006, India

    Jamuna Bai Aswathanarayan & Ravishankar Rai Vittal

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  1. Jamuna Bai Aswathanarayan
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  2. Ravishankar Rai Vittal
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Correspondence to Ravishankar Rai Vittal.

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Aswathanarayan, J.B., Vittal, R.R. Attachment and biofilm formation of Pseudomonas fluorescens PSD4 isolated from a dairy processing line. Food Sci Biotechnol 23, 1903–1910 (2014). https://doi.org/10.1007/s10068-014-0260-8

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  • DOI: https://doi.org/10.1007/s10068-014-0260-8

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