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Purification and characterization of a novel skatole-degrading protease from Lactobacillus brevis 1.12

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Abstract

Skatole, a substance with an unpleasant flavor, is released from the cecum and colon of entire male pigs. In this study, a novel skatole-degrading protease from the fermentation broth of Lactobacillus brevis 1.12 was extracted and purified through (NH4)2SO4 precipitation and chromatography with DEAE-Sephadex A-25 and Sephadex G-75. The purified protease was found to be homogeneous in terms of SDS-PAGE and had a molecular mass estimate of 57.5 kDa. This skatole-degrading protease had an optimal pH of 5.0 and optimal temperature of 35°C. The enzyme was only inhibited greatly at a 10 mmol 1/L concentration of K+. The results suggest that the novel skatole-degrading protease was purified. The biochemical characteristics of this protease can provide useful information for its potential use in industrial applications.

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  1. College of Food Science, Southwest University, Chongqing, 400715, China

    Xiao Meng, Zhi Fei He & Hong Jun Li

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  1. Xiao Meng
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Correspondence to Hong Jun Li.

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Meng, X., He, Z.F. & Li, H.J. Purification and characterization of a novel skatole-degrading protease from Lactobacillus brevis 1.12. Food Sci Biotechnol 22, 1–7 (2013). https://doi.org/10.1007/s10068-013-0224-4

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  • DOI: https://doi.org/10.1007/s10068-013-0224-4

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