Abstract
Genetically modified organisms (GMO) invade more and more the agricultural production in the world. Although there are no legislations on GM labeling and cultivation of GM crops in Tunisia, the present study aims to check the status of GMO in Tunisian market using qualitative and quantitative real time-PCR (QRT-PCR). Three-hundred-sixty five samples were collected and different DNA extraction methods were adapted and optimized. Specific primers targeting 35S promoter from Cauliflower mosaic virus (CaMV) and nopaline synthase terminator from Agrobacterium tumefaciens (At) were used for the detection of the GMO insert and Taxon specific primers for the detection of plant species. Validated Taqman® probes (EU-RL) targeting event specific regions of the maize events MON810, Bt11, and the soybean event RRS were used for the quantification studies. Seven food and feed products showed different amounts of RRS (1.9%), MON810 (2.1%), and Bt11 (1.6%). The results demonstrate for the first time the presence of GMO in Tunisian markets reinforcing the need for the development of accurate quantitative methods in routine analyses.
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Alderborn A, Sundström J, Soeria-Atmadja D, Sandberg M, Andersson HC, Hammerling U. Genetically modified plants for non-food or non-feed purposes: Straightforward screening for their appearance in food and feed. Food Chem. Toxicol. 48: 453–464 (2010)
James C. Global Status of Commercialized Biotech/GM Crops: 2011. ISAAA Brief No. 43. ISAAA, Ithaca, NY, USA (2011)
Bellocchi G, De Giacomo M, Foti N, Mazzara M, Palmaccio E, Savini C, Di Domenicantonio C, Onori R, van den Eede G. Testing the interaction between analytical modules: An example with Roundup Ready® soybean line GTS 40-3-2. BMC Biotechnol. 10: 55 (2010)
Vijayakumar KR, Asha M, Lalitha RG, Prakash V. Detection of genetically modified soya and maize: Impact of heat processing. Food Chem. 117: 514–521 (2009)
Pansiot J, Chaouachi M, Cavellini L, Romaniuk M, Ayadi M, Bertheau Y, Laval V. Development of two screening duplex PCR assays for genetically modified organism quantification using multiplex real-time PCR master mixes. Eur. Food Res. Technol. 232: 327–334 (2011)
Alishahi A, Farahmand H, Prieto N, Cozzolino D. Identification of transgenic foods using NIR spectroscopy. Spectrochim. Acta A 75: 1–7 (2010)
Remi A. Agricultural biotechnology: Does it work in Africa? pp. In: Workshop on GMOs for African agriculture: Challenges and opportunities. September 18, ASSAE Offices, Lynwood, South Africa. Academy of Science for South Africa, Lynwood, South Africa (2009)
Paarlberg R. GMO foods and crops: Africa’s choice. New Biotech. 27: 609–613 (2010)
Louanchi M, Salhi LN, Ghazi M. Preliminary detection of GMO in crops and food products. pp. In: Algeria in the Proceeding of the 1st Global Conference on GMO Analysis. June 24–27, Villa Erba, Como, Italy (2008)
El-Sanhoty R, Broll H, Grohmann L, Linke B, Spiegelberg A, Bögl KW, Zagon J. Genetically modified maize and soybean on the Egyptian food market. Mol. Nutr. Food Res. 46: 360–363 (2002)
Abdel-Mawgood AL, Gassem AM, Alsadon AA, Safar-Alghamdi S, Al-Doss AA. Monitoring of genetically modified food in Saudi Arabia. Afr. J. Food Sci. 8: 536–540 (2010)
Tinker NA, Fortin MG, Mather DE. Random amplified polymorphic DNA and pedigree relationship in spring barley. Theor. Appl. Genet. 85: 976–984 (1993)
Arumuganathan K, Earle ED. Nuclear DNA content of some important plant species. Plant Mol. Bio. Rep. 9: 208–218 (1991)
Chaouachi M, Giancola S, Romaniuk M, Laval V, Bertheau Y, Brunel D. A strategy for designing multi-taxa specific reference gene systems. Example of application-ppi phosphofructokinase (ppi-PPF) used for the detection and quantification of 3 taxa: Maize (Zea mays), cotton (Gossypium hirsutum), and rice (Oryza sativa). J. Agr. Food Chem. 55: 8003–8010 (2007)
Guillaume P, Gruère A. Review of international labeling policies of genetically modified food to evaluate India’s proposed rule. AgBioForum 10(1): 51–64 (2007)
Hernandez M, Duplan MN, Berthier G, Vaitilingom M, Hauser W, Freyer R, Pla M, Bertheau Y. Development and comparison of four real-time polymerase chain reaction systems for specific detection and quantification of Zea mays. J. Agr. Food Chem. 52: 4632–4637 (2004)
Su-Lan B, Xiaobo Z, Ligeng M, Wenjie Z, Liu-Min F, Ning W, Xing WD. A simple and reliable assay for detecting specific nucleotide sequences in plants using optical thin-film biosensor chips. Plant J. 10: 132–156 (2006)
Randhawa GJ, Singh M, Chhabra R, Sharma R. Qualitative and quantitative molecular testing methodologies and traceability systems for commercialized Bt cotton events and other Bt crops under field trials in India. Food Anal. Method. 3: 295–303 (2010)
Hernandez M, Esteve T, Pla M. Real-time polymerase chain reaction based assays for quantitative detection of barley, rice, sunflower, and wheat. J. Agr. Food Chem. 53: 7003–7009 (2005)
Litao Y, Aihu P, Junwei J, Jiayu D, Jianxiu C, Huang C, Chengmei Z, Dabing Z. Validation of a tomato-specific gene, LAT52, used as an endogenous reference gene in qualitative and real-time quantitative PCR detection of transgenic tomatoes. J. Agr. Food Chem. 53: 183–190 (2005)
Mayu I, Satomi Y, Hirohito Y, Katsuyuki H, Hideo K, Takashi K, Satoshi F, Hiroshi A, Tamio M, Akhihiro H. Development of taxonspecific sequences of common wheat for the detection of genetically modified wheat. J. Agr. Food Chem. 53: 6294–6300 (2005)
Jiauyu D, Junwei J, Litao Y, Haibo W, Chengmei Z, Wenxuan L, Dabing Z. Validation of a rice specific gene, sucrose phosphate synthase, used as the endogenous reference gene for qualitative and real-time quantitative PCR detection of transgenes. J. Agr. Food Chem. 52: 3372–3377 (2004)
James D, Schmidt AM, Wall E, Green M, Masri S. Reliable detection and identification of genetically modified maize, soybean, and canola by multiplex PCR analysis. J. Agr. Food Chem. 51: 5839–5834 (2003)
Fernandez S, Charles-Delobel C, Geldreich A, Berthier G, Boyer F, Collonier C, Coue-Philippe G, Dioloez A, Duplan MN, Kebdani N, Romaniuk M, Bertheau Y. Quantification of the 35S promoter in DNA extracts from genetically modified organisms using real-time polymerase chain reaction and specificity assessment on various genetically modified organisms. Part I: Operating procedure. J. AOAC Int. 88: 547–557 (2005)
Allmann M, Candrian U, Höfelein C, Lüthy J. Polymerase chain reaction (PCR): A possible alternative to immunochemical methods assuring safety and quality of food. Z. Lebensm. Unters. For. 196: 248–251 (1993)
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Chaouachi, M., Nabi, N., Hafsa, A.B. et al. Monitoring of genetically modified food and feed in the Tunisian market using qualitative and quantitative real-time PCR. Food Sci Biotechnol 22, 1161–1170 (2013). https://doi.org/10.1007/s10068-013-0198-2
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DOI: https://doi.org/10.1007/s10068-013-0198-2