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Alternative extraction technique for the quantitative analysis of succinic acid in a chitosan-succinic acid powder mixture using GC-MS

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Abstract

Typical solvent extraction of succinic acid from a chitosan-succinic acid powder mixture gave unsatisfactory yield (10.0%) due to the presence of intramolecular crosslinks between the chitosan amine groups and succinic acid carboxylic groups. An improved extraction method, which included hydrolysis of cross-links by KOH to liberate succinic acid from the matrix, afforded satisfactory yield (95.2±4.0%). Quantitative analysis of succinic acid in chitosan-succinic acid mixture was performed using GCMS and the optimized analysis method showed good validation in terms of specificity, linearity, accuracy (recovery rate), precision (inter-day and intra-day precision), limit of quantification (LOQ), and limit of detection (LOD).

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Authors and Affiliations

  1. Graduate School of Biotechnology & Institute of Life Sciences & Resources, Kyung Hee University, Yongin, Gyeonggi, 446-701, Korea

    Do-Gyeong Lee, Ji-Hae Park, Myung-Chong Song, In-Sik Chung & Nam-In Baek

  2. Health Functional Food Team, Ju Yeong NS Co., Ltd., Seoul, 137-063, Korea

    Jee-Hye Yun

  3. Department of Herbal Foodceutical Science, Daegu Haany University, Gyeongsan, Gyeongbuk, 712-715, Korea

    Eun-Mi Ahn

Authors
  1. Do-Gyeong Lee
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  2. Ji-Hae Park
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  3. Jee-Hye Yun
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  4. Myung-Chong Song
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  5. Eun-Mi Ahn
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  6. In-Sik Chung
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  7. Nam-In Baek
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Correspondence to Nam-In Baek.

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Lee, DG., Park, JH., Yun, JH. et al. Alternative extraction technique for the quantitative analysis of succinic acid in a chitosan-succinic acid powder mixture using GC-MS. Food Sci Biotechnol 20, 1609–1614 (2011). https://doi.org/10.1007/s10068-011-0222-3

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  • DOI: https://doi.org/10.1007/s10068-011-0222-3

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