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Linear and nonlinear Raman microspectroscopy: History, instrumentation, and applications

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Abstract

Through carrying out Raman spectroscopy under a microscope, we can observe the molecular distribution and dynamics of living cells in situ without staining or labeling. In general, Raman microscopy/microspectroscopy is classified into spontaneous Raman microscopy/microspectroscopy and nonlinear Raman microscopy/microspectroscopy. This review will first summarize the historical development of Raman microscopes, and will discuss in detail their instrumentation and applications.

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Authors and Affiliations

  1. Institute of Applied Physics, University of Tsukuba, Tsukuba, Ibaraki, 305-8573, Japan

    Hideaki Kano

  2. Department of Chemistry, School of Science, The University of Tokyo, Bunkyo, Tokyo, 113-0033, Japan

    Hiroki Segawa

  3. UMR CNRS No. 7252, Institut de Recherche XLIM, 87060, Limoges Cedex, France

    Philippe Leproux & Vincent Couderc

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  1. Hideaki Kano
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  2. Hiroki Segawa
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  4. Vincent Couderc
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Correspondence to Hideaki Kano.

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Kano, H., Segawa, H., Leproux, P. et al. Linear and nonlinear Raman microspectroscopy: History, instrumentation, and applications. OPT REV 21, 752–761 (2014). https://doi.org/10.1007/s10043-014-0123-9

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