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Evaluation of decellularization protocols for production of porcine small intestine submucosa for use in abdominal wall reconstruction

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Abstract

Background

Porcine-derived acellular biologic grafts are increasingly used in abdominal wall reconstruction and other soft tissue repairs. In a previous work, we have shown porcine small intestine submucosa (PSIS) exhibits clear advantages over porcine pericardium (PPC) and porcine acellular dermal matrix (PADM) in repairing full-thickness abdominal wall defects. In the present study, we aim to determine, quantify, and compare the effects of two most commonly used decellularization protocols on biomechanical and biocompatible properties of PSIS.

Materials and methods

After mechanical preparation, PSIS was treated with either alkaline and acid (AA) protocol or sodium dodecyl sulfate (SDS) protocol. Cellular content removal, preservation of matrix components, micro- and ultra- structures, and mechanical properties were compared. The host responses were evaluated using PSIS for repairing rat abdominal wall defects.

Results and conclusion

With regard to the absence of cellular contents, neatly arranged collagen fiber structures, better retention of growth factors, better mechanical strength, lower degrees of local and systemic inflammatory responses, higher degree of vascularization and tissue ingrowth, alkaline and acid protocol exhibits clear advantages over SDS protocol for the preparation of PSIS extracellular matrix.

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Funding

This work was supported by the National Natural Science Foundation of China (No. 81571827), Shanghai Rising-Star Program (No. 17QA1405600), and Shanghai Excellent Young Medical Talents Program (No. 2017YQ006).

Author information

Author notes

  1. Yunsheng Chai and Jian Xu contribute equally to this work.

Authors and Affiliations

  1. Division of Colorectal Surgery, Changzheng Hospital, Second Military Medical University, No. 415, Fengyang Road, Shanghai, 200003, People’s Republic of China

    Y. Chai, J. Zhang, Z. Hu & H. Zhou

  2. Department of Surgery, Shanghai Baoshan District Combining Traditional Chinese and Western Medicine Hospital, No. 181, Youyi Road, Shanghai, People’s Republic of China

    J. Xu

  3. Department of Gastrointestinal Surgery, Shuguang Hospital, Shanghai University of Traditional Chinese Medicine, No. 528, Zhangheng Road, Shanghai, People’s Republic of China

    Y. Zhang

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  1. Y. Chai
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  4. J. Zhang
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Contributions

HZ and ZH designed the study. YC, JX, and YZ performed experiments. JZ and HZ analyzed the data. HZ wrote the draft. ZH revised it critically. All the authors approved the version to be published.

Corresponding authors

Correspondence to Z. Hu or H. Zhou.

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Conflict of interest

The authors declare that there are no conflicts of interest.

Ethical approval

This study was approved by the Institutional Review Board at the Second Military Medical University.

Human and animal rights

The experiments were performed according to the Principles of Laboratory Animal Care formulated by the National Society for Medical Research and the Guide for the Care and Use of Laboratory Animals published by the national Institutes of Health (NIH publication No. 85-23, revised 1996), and all protocols were approved by the Animal Care and Use Committee of Second Military Medical University.

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Chai, Y., Xu, J., Zhang, Y. et al. Evaluation of decellularization protocols for production of porcine small intestine submucosa for use in abdominal wall reconstruction. Hernia 24, 1221–1231 (2020). https://doi.org/10.1007/s10029-019-01954-4

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  • DOI: https://doi.org/10.1007/s10029-019-01954-4

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