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Electrochemical myoglobin biosensor based on carbon ionic liquid electrode modified with Fe3O4@SiO2 microsphere

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Abstract

In this paper, a Fe3O4@SiO2 core-shell structure microsphere was synthesized and used to investigate the direct electron transfer of myoglobin (Mb) with a 1-butylpyridinium hexafluorophosphate based carbon ionic liquid electrode (CILE) as the substrate electrode. The mixture of Mb and Fe3O4@SiO2 microsphere could form an organic–inorganic composite, which was immobilized on the surface of CILE with a chitosan (CS) film. Cyclic voltammetric experiments indicated that a pair of well-defined quasi-reversible redox peaks appeared on CS/Mb-Fe3O4@SiO2/CILE with the formal peak potential (E 0′) located at −0.31 V (vs. saturated calomel electrode), which was corresponded to the electroactive center of Mb heme Fe(III)/Fe(II) redox couples. Direct electrochemical behaviors of Mb in CS-Fe3O4@SiO2 composite film were carefully investigated with the electrochemical parameters calculated. The CS/Mb-Fe3O4@SiO2/CILE showed good electrocatalytic behaviors to the reduction of trichloroacetic acid in the concentration range from 0.2 to 11.0 mmol L−1 with the detection limit of 0.18 mmol L−1 (3σ). Based on CS/Mb-Fe3O4@SiO2/CILE, a new third-generation reagentless electrochemical biosensor was constructed with higher sensitivity and reproducibility.

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Acknowledgments

We are grateful for the financial support of the National Natural Science Foundation of China (nos. 30901700, 50905096).

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Authors and Affiliations

  1. Department of Precision Instruments, Tsinghua University, Beijing, 100084, People’s Republic of China

    Xiaofeng Wang & Zheng You

  2. Department of Orthodontics, Beijing Stomatological Hospital, Beijing, 100050, People’s Republic of China

    Hailiang Sha

  3. College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao, 266042, People’s Republic of China

    Zhaolan Sun & Wei Sun

Authors
  1. Xiaofeng Wang
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  2. Zheng You
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  3. Hailiang Sha
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  4. Zhaolan Sun
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  5. Wei Sun
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Correspondence to Wei Sun.

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Wang, X., You, Z., Sha, H. et al. Electrochemical myoglobin biosensor based on carbon ionic liquid electrode modified with Fe3O4@SiO2 microsphere. J Solid State Electrochem 18, 207–213 (2014). https://doi.org/10.1007/s10008-013-2259-0

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  • DOI: https://doi.org/10.1007/s10008-013-2259-0

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