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A novel and simple biosensor based on poly(indoleacetic acid) film and its application for simultaneous electrochemical determination of dopamine and epinephrine in the presence of ascorbic acid

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Abstract

A novel and simple biosensor based on poly(indoleacetic acid) film-modified electrode (PIAA/CPE) was fabricated by electrochemical polymerization of indoleacetic acid on a carbon paste electrode (CPE) through cyclic voltammetry. The resulting electrode was characterized by scanning electron microscopy, and the electrochemical behaviors of dopamine (DA) and epinephrine (EP) at the electrode were studied. It was illustrated that PIAA/CPE had excellent electrochemical catalytic activities toward DA and EP. The anodic peak currents (I pa) were dramatically enhanced by about seven-fold for DA and ten times for EP at PIAA/CPE. Thus, the determinations of DA and EP were carried out using PIAA/CPE successfully. The linear responses were obtained in the range of 3.0 × 10−7∼7.0 × 10−4 and 1.0 × 10−6 ∼8.0 × 10−4 mol L−1 with the detection limits (3σ) of 1 × 10−7 and 4 × 10−7 mol L−1 corresponding with DA and EP, respectively. Moreover, the cathodic peaks of DA and EP were well-separated with a potential difference about 325 mV in pH 5.3 phosphate-buffered saline, so simultaneous determination of DA and EP was carried out in this paper. Additionally, the interference studies showed that the PIAA/CPE exhibited excellent selectivity in the presence of ascorbic acid (AA). With good selectivity and sensitivity, the present method has been successfully applied to the determination of DA and EP in pharmaceutical samples.

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Acknowledgments

This work was supported by the Shaanxi Provincial Natural Science Foundation for Young Scientist (No. 2009JQ2011), the Shaanxi Provincial Education Department Foundation (No. 08JK322), the Basic Research Foundation (No. JC1014), and Youth Scientist Foundation (No. RC0942) of Xi’an University of Architecture & Technology.

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Authors and Affiliations

  1. School of Science, Xi’an University of Architecture & Technology, Xi’an, Shaanxi, 710055, China

    Yuanzhen Zhou, Maofang He & Sheying Dong

  2. College of Chemistry and Materials Science, Northwest University, Xi’an, Shaanxi, 710069, China

    Maofang He

  3. Institute of Analytical Science/Shaanxi Provincial Key Laboratory of Electroanalytical Chemistry, Northwest University, Xi’an, Shaanxi, 710069, China

    Cancan Huang & Jianbin Zheng

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  1. Yuanzhen Zhou
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  2. Maofang He
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  3. Cancan Huang
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  4. Sheying Dong
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Correspondence to Yuanzhen Zhou.

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Zhou, Y., He, M., Huang, C. et al. A novel and simple biosensor based on poly(indoleacetic acid) film and its application for simultaneous electrochemical determination of dopamine and epinephrine in the presence of ascorbic acid. J Solid State Electrochem 16, 2203–2210 (2012). https://doi.org/10.1007/s10008-012-1646-2

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  • DOI: https://doi.org/10.1007/s10008-012-1646-2

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