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The immobilization of Cytochrome c on MWNT–PAMAM–Chit nanocomposite incorporated with DNA biocomposite film modified glassy carbon electrode for the determination of nitrite

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Abstract

A novel and sensitive biosensor was developed for the determination of nitrite. Firstly, multi-walled carbon nanotubes–poly(amidoamine)–chitosan (MWNT–PAMAM–Chit) nanocomposite along with the incorporation of DNA was used to modify the glassy carbon electrode. Then the immobilization of Cyt c was accomplished using electrochemical deposition method by consecutive cyclic voltammetry (CV) scanning in a neutral Cyt c solution. CV behaviors of the modified electrodes showed that the MWNT–PAMAM–Chit nanocomposite is a good platform for the immobilization of DNA and Cyt c in order, at the same time, an excellent promoter for the electron transfer between Cyt c and the electrode. At high potential, the immobilized Cyt c could be further oxidized into highly reactive Cyt c π-cation by two-step electrochemical oxidation, which could oxidize NO2 − into NO3 − in the solution. Therefore, a nitrite biosensor based on the biocatalytic oxidation of the immobilized Cyt c was fabricated, which showed a fast response to nitrite (less than 5 s). The linear range of 0.2–80 μM and a detection limit of 0.03 μM was obtained. Finally, the application in food analysis using sausage as testing samples was also investigated.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (No.20775044) and the Natural Science Foundation of Shandong province, China (Y2006B20).

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Authors and Affiliations

  1. College of Chemistry and Material Science, Shandong Agricultural University, Taian, Shandong, 271018, China

    Shiyun Ai, Hai Fan, Jun Cai, Qiang Ma & Huanshun Yin

  2. College of Resources and Environment, Shandong Agricultural University, Taian, Shandong, 271018, China

    Quanpeng Chen & Xiangbin Zhu

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  1. Quanpeng Chen
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  2. Shiyun Ai
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  3. Hai Fan
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  4. Jun Cai
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  7. Huanshun Yin
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Correspondence to Shiyun Ai.

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Chen, Q., Ai, S., Fan, H. et al. The immobilization of Cytochrome c on MWNT–PAMAM–Chit nanocomposite incorporated with DNA biocomposite film modified glassy carbon electrode for the determination of nitrite. J Solid State Electrochem 14, 1681–1688 (2010). https://doi.org/10.1007/s10008-010-1010-3

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