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Direct electrochemistry and biocatalysis of glucose oxidase immobilized on magnetic mesoporous carbon

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Abstract

A magnetic mesoporous carbon material (i.e., mesoporous iron oxide/C, mesoFe/C) is synthesized for protein immobilization, using glucose oxidase (GOx) as model. Transmission electron microscopy images show that mesoFe/C has highly ordered porous structure with uniform pore size, and iron oxide nanoparticles are dispersed along the wall of carbon. After adsorption of GOx, the GOx-mesoFe/C composite is separated with magnet. The immobilized GOx remains its natural structure according to the reflection–absorption infrared spectra. When the GOx-mesoFe/C composite is coated on a Pt electrode surface, the GOx gives a couple of quasireversible voltammetric peaks at −0.5 V (vs. saturated calomel electrode) due to the redox of FAD/FADH2. The electron-transfer rate constant (k s) is ca. 0.49 s−1. The modified electrode presents remarkably amperometric response to glucose at 0.6 V. The response time (t 95%) is less than 6 s; the response current is linear to glucose concentration in the range of 0.2–10 mM with a sensitivity of 27 μA mM−1 cm−2. The detection limit is 0.08 mM (S/N = 3). The apparent Michaelis–Menten constant (K appm ) of the enzyme reaction is ca. 6.6 mM, indicating that the GOx immobilized with mesoFe/C has high affinity to the substrate.

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Acknowledgments

The authors appreciate the support from the National Natural Science Foundation of China (grant number: 20173040) and the State Key Laboratory of Chem/Biosensing and Chemometrics, Hunan University, Changsha, People’s Republic of China.

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Authors and Affiliations

  1. Department of Chemistry, Wuhan University, Hubei Province, Wuhan, 430072, People’s Republic of China

    Jingjing Yu, Jiaxing Tu, Faqiong Zhao & Baizhao Zeng

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  1. Jingjing Yu
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  2. Jiaxing Tu
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  3. Faqiong Zhao
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  4. Baizhao Zeng
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Corresponding author

Correspondence to Faqiong Zhao.

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Table S1

Determination result of glucose in beer samples. (DOC 30 kb)

Fig. S1

a CVs of Nafion/GOx-mesoFe/C/Pt in PBS of different pH (from a to e: 4.92, 5.91, 6.98, 8.04, 9.18); b Plots of Epa vs. pH (a), formal potential vs. pH (b), and Epc vs. pH (c). (DOC 197 kb)

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Yu, J., Tu, J., Zhao, F. et al. Direct electrochemistry and biocatalysis of glucose oxidase immobilized on magnetic mesoporous carbon. J Solid State Electrochem 14, 1595–1600 (2010). https://doi.org/10.1007/s10008-009-0990-3

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  • DOI: https://doi.org/10.1007/s10008-009-0990-3

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