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Enzymatic formation of ions and their detection at a three-phase electrode

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Abstract

An electrochemical method for the detection of enzymatically created anions is described that uses a thin-film electrode with decamethylferrocene as an electroactive redox probe. The enzymatic oxidation of glucose with enzyme glucose oxidase produces gluconic acid as a final product. The oxidation of decamethylferrocene dissolved in the thin-nitrobenzene film, that is spread on the working graphite electrode and submerged in the aqueous solution containing glucose and glucose oxidase, is followed by the up-take of gluconate anions from the aqueous phase to nitrobenzene. The peak currents of the square-wave voltammetric responses of that system are a linear function of the glucose concentration in the milimolar range from 0.1 mmol/L to 0.7 mmol/L (R2=0.994).

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Acknowledgments

Rubin Gulaboski thanks Fundação para a Ciência e a Tecnologia (FCT) of Portugal for providing of a post. doc. fellowship (SFRH/BPD/14894/2004). The authors would like to thank FCT for financial support (CIQ L4 and FEDER for the project POCTI/QUI/42472/2001).

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Authors and Affiliations

  1. Departamento de Quimica, Faculdade do Ciências, Universidade do Porto, 4169-007, Porto, Portugal

    Rubin Gulaboski, Carlos M. Pereira, M. Natalia D. S. Cordeiro & Fernando Silva

  2. Institute of Physiology, University of Saarland, Homburg, Germany

    Ivan Bogeski

Authors
  1. Rubin Gulaboski
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  2. Carlos M. Pereira
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  3. M. Natalia D. S. Cordeiro
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  4. Ivan Bogeski
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  5. Fernando Silva
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Corresponding author

Correspondence to Carlos M. Pereira.

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Gulaboski, R., Pereira, C.M., Cordeiro, M.N.D.S. et al. Enzymatic formation of ions and their detection at a three-phase electrode. J Solid State Electrochem 9, 469–474 (2005). https://doi.org/10.1007/s10008-005-0696-0

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  • DOI: https://doi.org/10.1007/s10008-005-0696-0

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