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Biocompatibility of bulk-fill resins in vitro

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Abstract

Objectives

This study aims to evaluate the cytotoxicity and genotoxicity of three different extracts obtained from Filtek™ One Bulk Fill, Tetric Evoceram® Bulk Fill and Coltene Fill-Up! resins.

Materials and methods

The cytotoxicity was determined on 3T3 fibroblast cells using the MTT and crystal violet assays. The genotoxicity was determined using a cytokinesis-block micronucleus assay.

Results

The cytotoxicity of the resin extracts on 3T3 mouse fibroblasts was found to be dose-dependent with both the MTT and crystal violet assays. Extracts concentrated above 1% were cytotoxic according to the MTT assay. The Filtek™ One Bulk Fill, Tetric Evoceram® Bulk Fill, and Coltene Fill-Up! resins reached the LD50 at concentrations of 60%, 50%, and 20%, respectively, and showed genotoxicity rates that were 2–5 times, 3–8 times, and 4–15 times higher than the negative control, respectively.

Conclusions

Coltene Fill-Up! resin extracts were the most cytotoxic and genotoxic, followed by Tetric Evoceram® Bulk Fill and Filtek™ One Bulk Fill.

Clinical relevance

The analyzed bulk-fill resins showed differences in in vitro biocompatibility, and the Filtek™ One Bulk Fill was found to be the safest for clinical use.

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Funding

Egas Moniz CRL funded this research.

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Authors and Affiliations

  1. Egas Moniz Center for Interdisciplinary Research (CiiEM), Egas Moniz School of Health & Science, Quinta da Granja, Monte da Caparica, 2829-511, Caparica, Portugal

    Carla Junqueira, Paulo Mascarenhas, Mariana Avelar, Ana Clara Ribeiro & Isabel Barahona

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  1. Carla Junqueira
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  2. Paulo Mascarenhas
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  3. Mariana Avelar
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  4. Ana Clara Ribeiro
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  5. Isabel Barahona
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Contributions

C. J. and I. B. wrote the main manuscript and P. M. and M. A. prepared figures. All authors reviewed the manuscript.

Corresponding author

Correspondence to Isabel Barahona.

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Junqueira, C., Mascarenhas, P., Avelar, M. et al. Biocompatibility of bulk-fill resins in vitro. Clin Oral Invest 27, 7851–7858 (2023). https://doi.org/10.1007/s00784-023-05376-6

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