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Manganese oxide increases bleaching efficacy and reduces the cytotoxicity of a 10% hydrogen peroxide bleaching gel

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Abstract

Objective

The study aims to assess the effects of a 10% H2O2 bleaching gel with different MnO2 concentrations on the bleaching efficacy (BE), degradation kinetics (DK) of H2O2, and trans-amelodentinal cytotoxicity (TC).

Materials and methods

Standardized bovine enamel/dentin disks (n = 96) were placed in artificial pulp chambers, and the bleaching gels were applied for 45 min. Thus, the following groups were established: (G1) no treatment (negative control/NC); (G2) 35% H2O2 (positive control/PC); (G3) 10% H2O2; (G4) 10% H2O2 + 2 mg/mL MnO2; (G5) 10% H2O2 + 6 mg/mL MnO2; and (G6) 10% H2O2 + 10 mg/mL MnO2. After analyzing bleaching efficacy (ΔE00 and ΔWI), the degradation kinetics of H2O2 and trans-amelodentinal cytotoxicity were determined (n = 8, ANOVA/Tukey; p < 0.05).

Results

G6 presented BE (ΔE00 and ΔWI) statistically similar to G2, which represented conventional in-office bleaching (p = 0.6795; p > 0.9999). A significant reduction in the diffusion of H2O2 occurred in G3, G4, G5, and G6 compared to G2 (p < 0.0001). The highest DK of H2O2 occurred in G6 (p < 0.0001), which had the lowest TC in comparison with all other bleached groups (p ≤ 0.0186).

Conclusion

The addition of 10 mg/mL of MnO2 in a 10% H2O2 bleaching gel potentiates the degradation of this reactive molecule, which increases the BE of the product and decreases TC.

Clinical significance

Replacing a 35% H2O2 gel commonly used for conventional in-office dental bleaching by a 10% H2O2 gel containing 10 mg/mL of MnO2 reduces the cytotoxicity of this professional therapy, maintaining its excellent esthetic efficacy.

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Funding

The work was supported by the Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) (grants 2020/08882–6 and 2021/01184–4) and the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) (grants 302047/2019–0 and 408721/2018–9).

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Authors and Affiliations

  1. Department of Dental Materials and Prosthodontics, School of Dentistry, São Paulo State University (UNESP), Araraquara, Brazil

    Rafael Antonio de Oliveira Ribeiro, Uxua Ortecho Zuta & Igor Paulino Mendes Soares

  2. Department of Morphology and Pediatric Dentistry, School of Dentistry, São Paulo State University (UNESP), Araraquara, Brazil

    Caroline Anselmi & Josimeri Hebling

  3. Department of Dentistry, Endodontics and Dental Materials, School of Dentistry, São Paulo University (USP), Bauru, Brazil

    Diana Gabriela Soares

  4. Department of Restorative Dentistry, School of Dentistry, São Paulo State University (UNESP), Aracatuba, Brazil

    André Luiz Fraga Briso

  5. Department of Physiology and Pathology, School of Dentistry, São Paulo State University (UNESP), Araraquara, Brazil

    Carlos Alberto de Souza Costa

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  1. Rafael Antonio de Oliveira Ribeiro
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Correspondence to Carlos Alberto de Souza Costa.

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de Oliveira Ribeiro, R.A., Zuta, U.O., Soares, I.P.M. et al. Manganese oxide increases bleaching efficacy and reduces the cytotoxicity of a 10% hydrogen peroxide bleaching gel. Clin Oral Invest 26, 7277–7286 (2022). https://doi.org/10.1007/s00784-022-04688-3

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