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Volumetric analysis of the periodontal microstructure under antiresorptive therapy: an experimental study in rabbits

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Abstract

Objectives

The objective of this study was to volumetrically assess changes in the periodontal microstructure under antiresorptive therapy.

Materials and methods

Microtomographic scans from a total of 9 Dutch Belted rabbits having been randomly allocated to either the intravenous administration of amino-bisphosphonate (zoledronic acid) (Za) (n = 5) or a negative control group (nZa) (n = 4) were obtained at 10 months following a repeated drug administration. A quantification of the periodontal space thickness (P.Th) of both maxillary and mandibular most posterior premolars, as well as of the 2nd molars was performed. Bone micromorphometry was assessed by means of bone volume per total volume (BV/TV), the bone mineral density (BMD), trabecular thickness (Tb.Th), trabecular number (Tb.N), bone surface (BS), and the specific bone surface (BS/BV).

Results

Za was associated with significantly higher P.Th (P = 0.010), which was most pronounced in the upper jaw. Bone micromorphometry revealed no significant differences among the two groups, i.e., Za and nZa, for all the investigated parameters.

Conclusions

Volumetric analysis revealed that antiresorptive therapy was associated with periodontal space widening, whereas major effects on the bone micro-morphology could not be observed.

Clinical relevance

A deep understanding of specific periodontal and alveolar bone alterations in patients under antiresorptive therapy might help to prevent the onset of medication-related osteonecrosis of the jaw.

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Data availability

Data will be provided upon reasonable request.

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Acknowledgements

We kindly appreciate the competent assistance of Prof. Dr. Martin Sager and Ms. Iris Schrey (ZETT Institute, Heinrich Heine University, Düsseldorf, Germany).

Funding

The analysis was funded by a grant (14–007) of the Osteology Foundation, Lucerne, Switzerland. The funder had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript; and in the decision to publish the results.

Author information

Author notes

  1. Kathrin Becker, Robert Kerberger, and Giulia Brunello contributed equally to this work.

Authors and Affiliations

  1. Department for Orthodontics, Universitätsklinikum Düsseldorf, Düsseldorf, Germany

    Kathrin Becker, Robert Kerberger & Joel Rockhoff

  2. Department of Oral Surgery, Universitätsklinikum Düsseldorf, Düsseldorf, Germany

    Giulia Brunello & Nicole Rauch

  3. Department of Neuroscience, Dental School, University of Padova, Padova, Italy

    Giulia Brunello

  4. Department of Oral Surgery and Implantology, Goethe University, Carolinum, Frankfurt, Germany

    Frank Schwarz

Authors
  1. Kathrin Becker
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  2. Robert Kerberger
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  3. Giulia Brunello
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  4. Joel Rockhoff
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  5. Nicole Rauch
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  6. Frank Schwarz
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Contributions

The authors contributions of the present research were the following: F.S. and K.B. conceived the ideas; K.B., R.K., J.R., N.R., and G.B. collected and analyzed the data; and F.S. and K.B. led the writing. All authors critically revised the manuscript and agreed to the final version.

Corresponding author

Correspondence to Frank Schwarz.

Ethics declarations

Ethics approval

The study protocol was approved by the appropriate local authority (Landesamt für Natur und Verbraucherschutz, Recklinghausen, Germany) and was performed in compliance with the European Directive 2010/63 on the protection of animals used for scientific purposes, ISO 10993–2 (2006) and Good Laboratory Practice regulations.

Consent to participate

Not applicable for research involving animals.

Conflict of interest

Prof. F. Schwarz declares to have received grants from the Osteology Foundation, to have been supported by Geistlich Biomaterials and Osteology Foundation for scientific presentations at conferences and educational events, and to be an Executive Board Member of the Osteology Foundation. Dr. Kathrin Becker has been a speaker at Osteology Research Academy events and is a member of the Osteology Scientific Review Board. The other authors declare that they have no conflict of interests related to this study.

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Becker, K., Kerberger, R., Brunello, G. et al. Volumetric analysis of the periodontal microstructure under antiresorptive therapy: an experimental study in rabbits. Clin Oral Invest 26, 5359–5368 (2022). https://doi.org/10.1007/s00784-022-04503-z

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