Abstract
Objectives
The objective of this study is to formulate experimental dental adhesives with different polyhexamethylene guanidine hydrochloride concentrations (PHMGH) and evaluate their physical, chemical, and biological properties.
Materials and methods
The experimental adhesives were formulated with 0 (control, GCTRL), 0.5 (G0.5%), 1 (G1%), or 2 (G2%) wt.% into the adhesive. The adhesives were analyzed for degree of conversion (DC%), softening in solvent (ΔKHN%), ultimate tensile strength (UTS), microtensile bond strength (μTBS) immediately and after 1 year of aging, antibacterial activity, and cytotoxicity.
Results
There were no differences among groups for DC%, ΔKHN%, and UTS (p > 0.05%). There were no differences between each PHMGH-doped adhesive compared to GCTRL in the immediate μ-TBS (p > 0.05). Adhesives with at least 1 wt.% of PHMGH presented better stability of μ-TBS. PHMGH-doped adhesives showed improved longitudinal μ-TBS compared to GCTRL (p < 0.05). Lower Streptococcus mutans biofilm formation was observed for PHMGH-doped adhesives (p < 0.05). There was lower viability of planktonic S. mutans in the media in contact with the samples when at least 1 wt.% of PHGMGH was incorporated (p < 0.05). The formulated adhesives showed no cytotoxicity against pulp cells (p > 0.05).
Conclusions
The adhesive with 2 wt.% of PHMGH showed the highest antibacterial activity, without affecting the physicochemical properties and cytotoxicity, besides conferring stability for the dental adhesion.
Clinical relevance
PHMGH, a positively charged polymer, conveyed antibacterial activity to dental adhesives. Furthermore, it did not negatively affect the essential physicochemical and biocompatibility properties of the adhesives. More importantly, the incorporation of PHMGH provided stability for the μ-TBS compared to the control group without this additive.
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Acknowledgments
The authors acknowledge the CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico) for the scholarship of L.B.S.
Funding
This study was financed in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - Brasil (CAPES) - Finance Code 001.
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L.B.S. contributed to the formal analysis, investigation, writing — original draft preparation. I.M.G. contributed to the conception, formal analysis, investigation, visualization, writing — review and editing. F.V. contributed to formal analysis, investigation. F.M.C. contributed to the conception, formal analysis, supervision, investigation. V.C.B.L contributed to conception, investigation, formal analysis, supervision, visualization, writing — review and editing. All authors read and approved the final manuscript.
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All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki Declaration and its later amendments or comparable ethical standards. The study was approved by the Local Ethical Committee of the Federal University of Rio Grande do Sul.
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Silvestrin, L.B., Garcia, I.M., Visioli, F. et al. Physicochemical and biological properties of experimental dental adhesives doped with a guanidine-based polymer: an in vitro study. Clin Oral Invest 26, 3627–3636 (2022). https://doi.org/10.1007/s00784-021-04332-6
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DOI: https://doi.org/10.1007/s00784-021-04332-6