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Captopril inhibits matrix metalloproteinase activity and improves dentin bonding durability

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Abstract

Objectives

We investigated the inhibitory effects of captopril on matrix metalloproteinases (MMPs) and its effect as a primer on dentin bonding durability.

Materials and methods

One hundred fifty human third molars were selected. Flat surfaces of the middle dentin were exposed, etched 15 s, and followed by pretreatment with a primer for 60 s, including distilled water (control, the negative control primer), 2% chlorhexidine digluconate (CHD, the positive control primer), and captopril solution. Inhibitory effects of primers on MMPs were evaluated by hydroxyproline and gelatinase activity tests. All primers were applied on dentin followed by bonding. Some of the samples were sliced into slabs, placed in a fluorescent solution containing gelatin, and incubated for in situ zymography. Some were cut into sticks, and after aging for 1 day, 12 months, or 24 months, microtensile bonding strength was tested. Some were cut into slabs, aged for 1 day, 12 months, or 24 months, and taken out for nanoleakage tests to reveal interface defects.

Results

Hydroxyproline and gelatinase activity analyses showed that captopril exerted better inhibitory effects on MMPs, relative to 2% CHD (p < 0.05). A 0.2% captopril aqueous solution (0.2% CapW) was chosen to apply to the dentin. In situ zymography showed that inhibitory effects of captopril on gelatinase were significantly higher compared to 2% CHD (p < 0.01). Microtensile strength revealed that the bonding effects of the 0.2% CapW group lasted longer, compared to the control and 2% CHD groups (p < 0.05). Interface defects, detected by nanoleakage, were significantly reduced in the 0.2% CapW group, compared to the control and 2% CHD groups (p < 0.05).

Conclusions

Captopril inhibits dentin MMP activities and effectively improves dentin bonding durability.

Clinical relevance

Captopril is a promising dentin bonding primer for improving bonding durability.

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Acknowledgements

The authors thank Dandan Song in the Center of Cryo-Electron Microscopy (CCEM), Zhejiang University, for their technical assistance on scanning electron microscopy, transmission electron microscopy, and Cryo-TEM. The authors thank the technical support by Bio-ultrastructure Analysis Laboratory of Analysis, Center of Agrobiology and Environmental Sciences, Zhejiang University.

Funding

This work was funded by the Educational Department Foundation of Zhejiang Province of China (N20120554).

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Author notes

  1. Chang Shu and Xinyu Zheng contributed equally to this work.

Authors and Affiliations

  1. Stomatology Hospital, School of Stomatology, Zhejiang University School of Medicine, Zhejiang Provincial Clinical Research Center for Oral Disease, Key Laboratory of Oral Biomedical Research of Zhejiang Province, Cancer Center of Zhejiang University, Hangzhou, 310006, People’s Republic of China

    Chang Shu, Yang Wang, Yi Xu, Denghui Zhang & Shuli Deng

  2. Department of Stomatology, Zhejiang University Hospital, Zhejiang University, Hangzhou, People’s Republic of China

    Xinyu Zheng

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  1. Chang Shu
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Contributions

Conceptualization, Chang Shu, Xinyu Zheng, and Shuli Deng; methodology, Chang Shu, Xinyu Zheng, Yang Wang, Yi Xu, and Denghui Zhang; formal analysis and investigation, Chang Shu, Yang Wang, Yi Xu, and Denghui Zhang; writing — original draft preparation, Chang Shu; writing — review and editing, Chang Shu, Xinyu Zheng, and Shuli Deng; funding acquisition, Xinyu Zheng and Shuli Deng; supervision, Shuli Deng.

Corresponding author

Correspondence to Shuli Deng.

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This study was performed in line with the principles of the Declaration of Helsinki. Approval was granted by the Institutional Ethics Committee of Stomatology Hospital, School of Stomatology, Zhejiang University School of Medicine, China (number 2018010).

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Informed consent was obtained from all individual participants included in the study.

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Shu, C., Zheng, X., Wang, Y. et al. Captopril inhibits matrix metalloproteinase activity and improves dentin bonding durability. Clin Oral Invest 26, 3213–3225 (2022). https://doi.org/10.1007/s00784-021-04303-x

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