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Genetic variants in tooth agenesis–related genes might be also involved in tooth size variations

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Abstract

Objective

The present study aimed to evaluate if genetic variants in PAX9, MSX1, TGFα, FGF3, FGF10, FGF13, GLI2 and GLI3 are involved in TS of permanent teeth.

Materials and methods

Pretreatment dental records from orthodontic patients were assessed prior to recruitment. Patients with tooth agenesis and congenital anomalies (including oral cleft) and/or syndromes were excluded. Dental casts were used to measure the maximum crown dimensions of all fully erupted permanent teeth except second and third molars in mesiodistal direction. Teeth with caries, occlusal wear, mesiodistal restorations, and obvious deformities were not evaluated. Genomic DNA samples were used for genotyping. The allelic discrimination of 13 genetic variants was performed. The associations between TS and genotype were analyzed by linear regression, adjusted by gender at a significance level of p ≤ 0.05.

Results

Genetic polymorphisms in the tooth agenesis–related genes studied here were associated with increased and decreased TS, in both maxilla and mandible (p < 0.05).

Conclusion

This study reported associations of novel tooth agenesis–related gene variants with permanent tooth size variations.

Clinical relevance

The presence of some genetic variants could allow the prediction of permanent tooth size.

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Funding

This work was supported by the São Paulo Research Foundation (FAPESP) (funding number: 2015/06866-5) and individual scholarships (FAPESP 2018/03533-3, 2017/16990-0 and 2016/08149-1, and CAPES).

Author information

Authors and Affiliations

  1. Department of Pediatric Dentistry, School of Dentistry of Ribeirão Preto, University of São Paulo, Avenida do Café s/n – Campus da USP, Sao Paulo, 4040-904, Brazil

    Arthur S. Cunha, Luiza Vertuan dos Santos, Guido Artemio Marañón-Vásquez, Maria Bernadete Stuani, Mírian Aiko Nakane Matsumoto & Erika Calvano Küchler

  2. Department of Orthodontics, University Medical Centre of Regensburg, Franz-Josef-Strauss-Allee 11, 93053, Regensburg, Germany

    Christian Kirschneck

  3. School of Health Science, Positivo University, Curitiba, Brazil

    Jennifer Tsi Gerber

  4. Department of Oral Biology, School of Dental Medicine, University of Pittsburgh, 412 Salk Pavilion, 335 Sutherland Street, Pittsburgh, PA, 15261, USA

    Alexandre Rezende Vieira

  5. Department of Stomatology, Federal University of Parana, Curitiba, Paraná, Brazil

    Rafaela Scariot

Authors
  1. Arthur S. Cunha
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  2. Luiza Vertuan dos Santos
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  4. Christian Kirschneck
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  5. Jennifer Tsi Gerber
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  6. Maria Bernadete Stuani
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  10. Erika Calvano Küchler
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Contributions

ASC performed the genotype analysis and wrote the manuscript.

LVS performed the tooth measurements.

GAMV performed the DNA extraction and wrote the manuscript.

CK conceptualized and designed the study and wrote the manuscript.

JTSG performed the statistical analysis and wrote the results.

MBS designed the clinical part of the study and calibrated the operator to tooth measurements.

MANM patients’ recruitment, organized the sample and the data.

ARV provided funding support and led the PCR analysis.

RS designed and performed the statistical analysis.

ECK managed the funding resource, conceptualized and designed the study, mentored the students, and wrote the manuscript. All the authors read, corrected, and approved the final version of the manuscript.

Corresponding authors

Correspondence to Arthur S. Cunha or Erika Calvano Küchler.

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The authors declare that they have no conflict of interest.

Ethical approval

All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

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Informed consent was obtained from all individual participants included in the study.

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Cunha, A.S., dos Santos, L.V., Marañón-Vásquez, G.A. et al. Genetic variants in tooth agenesis–related genes might be also involved in tooth size variations. Clin Oral Invest 25, 1307–1318 (2021). https://doi.org/10.1007/s00784-020-03437-8

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