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Molecular imaging assessment of periodontitis lesions in an experimental mouse model

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Abstract

Objective

We aimed to evaluate molecular imaging as a novel diagnostic tool for mice periodontitis model induced by ligature and Porphyromonas gingivalis (Pg) inoculation.

Materials and methods

Twelve female mice were assigned to the following groups: no treatment as control group (n = 4); periodontitis group induced by ligature and Pg as Pg group (n = 4); and Pg group treated with glycyrrhizinic acid (GA) as Pg + GA group (n = 4). All mice were administered a myeloperoxidase (MPO) activity-specific luminescent probe and observed using a charge-coupled device camera on day 14. Image analysis on all mice was conducted using software to determine the signal intensity of inflammation. Additionally, histological and radiographic evaluation for periodontal inflammation and bone resorption at the site of periodontitis, and quantitative enzyme-linked immunosorbent assay (ELISA) were conducted on three mice for each group. Each experiment was performed three times.

Results

Levels of serum IgG antibody against P. gingivalis were significantly higher in the Pg than in the Pg + GA group. Histological analyses indicated that the number of osteoclasts and neutrophils were significantly lower in the Pg + GA than in the Pg group. Micro-CT image analysis indicated no difference in bone resorption between the Pg and Pg + GA groups. The signal intensity of MPO activity was detected on the complete craniofacial image; moreover, strong signal intensity was localized specifically at the periodontitis site in the ex vivo palate, with group-wise differences.

Conclusions

Molecular imaging analysis based on MPO activity showed high sensitivity of detection of periodontal inflammation in mice.

Clinical relevance

Molecular imaging analysis based on MPO activity has potential as a diagnostic tool for periodontitis.

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Acknowledgements

The authors would like to thank the staff at the Okayama Medical Innovation Center and Public Laboratory at Okayama University for their support in the molecular imaging study. The authors would also like to thank Dr. Mitsuaki Ono and the Department of Oral Rehabilitation and Regenerative Medicine at Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences for their support in the micro-CT scanning.

Funding

This work was funded by a JSPS KAKENHI grant-in-aid for Young Scientists (B) Number JP24792327, the Kobayashi Magobe Memorial Medical Foundation, the Ryobi Teien Foundation, and a grant-in-aid for the COE projects by MEXT, Japan, titled “Center of excellence for molecular and gene targeting therapies with micro-doze molecular imaging modalities.”

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Authors and Affiliations

  1. Department of Pathophysiology - Periodontal Science, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, 2-5-1 Shikata-cho, Kita-ku, Okayama, 700-8525, Japan

    Hidetaka Ideguchi, Keisuke Yamashiro, Tadashi Yamamoto, Masayuki Shimoe, Shoichi Hongo, Shinsuke Kochi, Chiaki Yoshihara-Hirata, Hiroaki Aoyagi, Mari Kawamura & Shogo Takashiba

Authors
  1. Hidetaka Ideguchi
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  2. Keisuke Yamashiro
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  3. Tadashi Yamamoto
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  4. Masayuki Shimoe
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  5. Shoichi Hongo
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  6. Shinsuke Kochi
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  7. Chiaki Yoshihara-Hirata
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  8. Hiroaki Aoyagi
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  9. Mari Kawamura
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  10. Shogo Takashiba
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Corresponding author

Correspondence to Shogo Takashiba.

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This article does not contain any studies with human participants performed by any of the authors.

Conflict of interest

The authors declare that they have no conflicts of interest.

Ethical approval

All animal experimental protocols and procedures were approved by the Animal Care and Use Committee, Okayama University, Japan (#OKU-2014459).

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For this type work, formal consent is not required.

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Ideguchi, H., Yamashiro, K., Yamamoto, T. et al. Molecular imaging assessment of periodontitis lesions in an experimental mouse model. Clin Oral Invest 23, 821–827 (2019). https://doi.org/10.1007/s00784-018-2510-2

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  • DOI: https://doi.org/10.1007/s00784-018-2510-2

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