Skip to main content

Advertisement

SpringerLink
Log in

Influence of CAD/CAM all-ceramic materials on cell viability, migration ability and adenylate kinase release of human gingival fibroblasts and oral keratinocytes

  • Original Article
  • Published:
Clinical Oral Investigations Aims and scope Submit manuscript

Abstract

Objectives

The aim of this study was to analyze the influence of four CAD/CAM all-ceramic materials on cell viability, migration ability and adenylate kinase (ADK) release of human gingival fibroblasts (HGF) and oral keratinocytes (HOK).

Materials and methods

HGF and HOK were cultured on disc-shaped CAD/CAM all-ceramic materials (e.max CAD LT, e.max CAD HT, Empress CAD and Mark II) and on discs made of tissue culture polystyrene surface (TCPS) serving as control. Cell viability was analyzed by using an MTT assay, and migration ability was investigated by a scratch assay. A ToxiLight assay has been performed to analyze the effect of all-ceramic materials on ADK release and cell apoptosis.

Results

At MTT assay for HGF, no significant decrease of cell viability could be detected at all points of measurement (p each > 0.05), while HOK demonstrated a significant decrease in cell viability especially on Empress CAD and Mark II at each point of measurement (p each < 0.001). Scratch assay demonstrated an increased migration ability for HGF on e.max CAD HT, Empress CAD and Mark II (p each < 0.001), whereas HOK showed a significantly decreased migration ability on all tested materials at all points of measurement (between −36 % and −71 %; p each < 0.001). At ToxiLight assay, only small cytotoxic effects of the all-ceramic materials could be investigated.

Conclusions

This study disclosed significant differences in cell viability and migration ability of HGF and HOK on CAD/CAM all-ceramic materials.

Clinical Relevance

CAD/CAM all-ceramic materials can influence oral cell lines responsible for soft tissue creation which may affect the esthetic outcome.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

References

  1. Christensen GJ (2003) The confusing array of tooth-colored crowns. J Am Dent Assoc 134:1253–1255

    Article  PubMed  Google Scholar 

  2. Belser UC, MacEntee MI, Richter WA (1985) Fit of three porcelain-fused-to-metal marginal designs in vivo: a scanning electron microscope study. J Prosthet Dent 53:24–29

    Article  PubMed  Google Scholar 

  3. Bindl A, Mormann WH (1999) Clinical evaluation of adhesively placed Cerec endo-crowns after 2 years—preliminary results. J Adhes Dent 1:255–265

    PubMed  Google Scholar 

  4. Bindl A, Mormann WH (2007) Fit of all-ceramic posterior fixed partial denture frameworks in vitro. Int J Periodont Restor Dent 27:567–575

    Google Scholar 

  5. Giordano R (2006) Materials for chairside CAD/CAM-produced restorations. J Am Dent Assoc 137(Suppl):14S–21S

    Article  PubMed  Google Scholar 

  6. Haselton DR, Diaz-Arnold AM, Hillis SL (2000) Clinical assessment of high-strength all-ceramic crowns. J Prosthet Dent 83:396–401

    Article  PubMed  Google Scholar 

  7. Martin N, Jedynakiewicz NM (1999) Clinical performance of CEREC ceramic inlays: a systematic review. Dent Mater 15:54–61

    Article  PubMed  Google Scholar 

  8. Akbar JH, Petrie CS, Walker MP, Williams K, Eick JD (2006) Marginal adaptation of Cerec 3 CAD/CAM composite crowns using two different finish line preparation designs. J Prosthodont 15:155–163

    Article  PubMed  Google Scholar 

  9. Beuer F, Aggstaller H, Edelhoff D, Gernet W, Sorensen J (2009) Marginal and internal fits of fixed dental prostheses zirconia retainers. Dent Mater 25:94–102

    Article  PubMed  Google Scholar 

  10. Bindl A, Mormann WH (2005) Marginal and internal fit of all-ceramic CAD/CAM crown-copings on chamfer preparations. J Oral Rehabil 32:441–447

    Article  PubMed  Google Scholar 

  11. Kokubo Y, Ohkubo C, Tsumita M, Miyashita A, Vult von Steyern P, Fukushima S (2005) Clinical marginal and internal gaps of Procera AllCeram crowns. J Oral Rehabil 32:526–530

    Article  PubMed  Google Scholar 

  12. Lee KB, Park CW, Kim KH, Kwon TY (2008) Marginal and internal fit of all-ceramic crowns fabricated with two different CAD/CAM systems. Dent Mater J 27:422–426

    Article  PubMed  Google Scholar 

  13. Luthardt RG, Bornemann G, Lemelson S, Walter MH, Huls A (2004) An innovative method for evaluation of the 3-D internal fit of CAD/CAM crowns fabricated after direct optical versus indirect laser scan digitizing. Int J Prosthodont 17:680–685

    PubMed  Google Scholar 

  14. Nakamura T, Dei N, Kojima T, Wakabayashi K (2003) Marginal and internal fit of Cerec 3 CAD/CAM all-ceramic crowns. Int J Prosthodont 16:244–248

    PubMed  Google Scholar 

  15. Reich S, Wichmann M, Nkenke E, Proeschel P (2005) Clinical fit of all-ceramic three-unit fixed partial dentures, generated with three different CAD/CAM systems. Eur J Oral Sci 113:174–179

    Article  PubMed  Google Scholar 

  16. Sailer I, Feher A, Filser F, Gauckler LJ, Luthy H, Hammerle CH (2007) Five-year clinical results of zirconia frameworks for posterior fixed partial dentures. Int J Prosthodont 20:383–388

    PubMed  Google Scholar 

  17. Seo D, Yi Y, Roh B (2009) The effect of preparation designs on the marginal and internal gaps in Cerec3 partial ceramic crowns. J Dent 37:374–382

    Article  PubMed  Google Scholar 

  18. Wataha JC (2000) Biocompatibility of dental casting alloys: a review. J Prosthet Dent 83:223–234

    Article  PubMed  Google Scholar 

  19. Wataha JC (2002) Alloys for prosthodontic restorations. J Prosthet Dent 87:351–363

    Article  PubMed  Google Scholar 

  20. Wataha JC, Nakajima H, Hanks CT, Okabe T (1994) Correlation of cytotoxicity with element release from mercury- and gallium-based dental alloys in vitro. Dent Mater 10:298–303

    Article  PubMed  Google Scholar 

  21. Wataha JC, Rueggeberg FA, Lapp CA, Lewis JB, Lockwood PE, Ergle JW et al (1999) In vitro cytotoxicity of resin-containing restorative materials after aging in artificial saliva. Clin Oral Investig 3:144–149

    Article  PubMed  Google Scholar 

  22. Nicholson JW, Czarnecka B (2008) The biocompatibility of resin-modified glass-ionomer cements for dentistry. Dent Mater 24:1702–1708

    Article  PubMed  Google Scholar 

  23. Roggendorf MJ, Kunzi B, Ebert J, Roggendorf HC, Frankenberger R, Reich SM (2012) Seven-year clinical performance of CEREC-2 all-ceramic CAD/CAM restorations placed within deeply destroyed teeth. Clin Oral Investig 16:1413–1424

    Article  PubMed  Google Scholar 

  24. Alfarsi MA, Okutan HM, Bickel M (2009) CAD/CAM to fabricate ceramic implant abutments and crowns: a preliminary in vitro study. Aust Dent J 54:12–16

    Article  PubMed  Google Scholar 

  25. Stadelmann WK, Digenis AG, Tobin GR (1998) Physiology and healing dynamics of chronic cutaneous wounds. Am J Surg 176:26–38

    Article  Google Scholar 

  26. Tao H, Berno AJ, Cox DR, Frazer KA (2007) In vitro human keratinocyte migration rates are associated with SNPs in the KRT1 interval. PLoS ONE 2:697

    Article  Google Scholar 

  27. Nebe B, Forster C, Pommerenke H, Fulda G, Behrend D, Bernewski U et al (2001) Structural alterations of adhesion mediating components in cells cultured on poly-beta-hydroxy butyric acid. Biomaterials 22:2425–2434

    Article  PubMed  Google Scholar 

  28. Anselme K, Bigerelle M, Noel B, Dufresne E, Judas D, Iost A, Hardouin P (2000) Qualitative and quantitative study of human osteoblast adhesion on materials with various surface roughnesses. J Biomed Mater Res 49:155–166

    Article  PubMed  Google Scholar 

  29. Sykaras N, Iacopino AM, Marker VA, Triplett RG, Woody RD (2000) Implant materials, designs, and surface topographies: their effect on osseointegration. A literature review. Int J Oral Maxillofac Implants 15:675–690

    PubMed  Google Scholar 

  30. Motro PF, Kursoglu P, Kazazoglu E (2012) Effects of different surface treatments on stainability of ceramics. J Prosthet Dent 108:231–237

    Article  PubMed  Google Scholar 

  31. Mustafa K, Wennerberg A, Arvidson K, Messelt EB, Haag P, Karlsson S (2008) Influence of modifying and veneering the surface of ceramic abutments on cellular attachment and proliferation. Clin Oral Implants Res 19:1178–1187

    Article  PubMed  Google Scholar 

  32. Pendegrass CJ, Gordon D, Middleton CA, Sun SN, Blunn GW (2008) Sealing the skin barrier around transcutaneous implants: in vitro study of keratinocyte proliferation and adhesion in response to surface modifications of titanium alloy. J Bone Joint Surg Br 90:114–121

    Article  PubMed  Google Scholar 

  33. Sommer S, Ackermann G (1986) Quantitative characterization of procedures using ultraviolett and visible molecular absorption spectrophotometry. Pure Appl Chem 58:1015–1022

    Article  Google Scholar 

  34. Mosmann T (1983) Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 65:55–63

    Article  PubMed  Google Scholar 

Download references

Conflict of interests

Dr. Karl-Martin Lehmann is a paid consultant of the VITA Zahnfabrik H. Rauter. There are no further conflicts of interests to this study. No fundings were received to this study.

Author information

Authors and Affiliations

  1. Department of Oral and Maxillofacial Surgery, University Medical Center Mainz, Augustusplatz 2, 55131, Mainz, Germany

    A M Pabst, C Walter & T Ziebart

  2. Department of Prosthetic Dentistry, University Medical Center Mainz, Augustusplatz 2, 55131, Mainz, Germany

    L Grassmann, M Weyhrauch, H Scheller & K M Lehmann

  3. Department of Oral Surgery, University Medical Center Mainz, Augustusplatz 2, 55131, Mainz, Germany

    D D Brüllmann

Authors
  1. A M Pabst
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. C Walter
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. L Grassmann
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. M Weyhrauch
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. D D Brüllmann
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. T Ziebart
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. H Scheller
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. K M Lehmann
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to K M Lehmann.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Pabst, A.M., Walter, C., Grassmann, L. et al. Influence of CAD/CAM all-ceramic materials on cell viability, migration ability and adenylate kinase release of human gingival fibroblasts and oral keratinocytes. Clin Oral Invest 18, 1111–1118 (2014). https://doi.org/10.1007/s00784-013-1098-9

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00784-013-1098-9

Keywords

Search

Navigation