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Murine osteoblasts respond to LPS and IFN-γ similarly to macrophages

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Abstract

Osteoblasts are bone-forming mesenchymal cells, while macrophages are cells of hematopoietic origin responsible for innate immunity. Lipopolysaccharide (LPS) can induce tolerance in macrophages, whereas interferon (IFN)-γ can activate macrophages to produce cytokines, exert bactericidal effects, and present antigens. In this study, we examined such macrophagic phenotypes regulated by LPS and IFN-γ in murine osteoblasts. In both primary calvarial osteoblasts and osteoblastic MC3T3-E1 cells, LPS pretreatment resulted in reduced production of IL-6 in response to a subsequent LPS stimulation or to Salmonella infection, indicating the existence of LPS-induced tolerance in osteoblasts. Furthermore, IFN-γ treatment of MC3T3-E1 cells resulted in both enhanced IL-6 production in response to LPS and upregulation of major histocompatibility complex class II (MHC II). Following infection, Salmonella-containing vacuoles (SCVs) were formed in MC3T3-E1 cells, and IFN-γ pretreatment enhanced bactericidal effects on intracellular Salmonella. Taken together, these observations indicate that osteoblasts can exhibit a subset of phenotypes reminiscent of macrophages in the course of bacterial infection.

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Authors and Affiliations

  1. Department of Microbiology and Immunology, School of Medicine, Keio University, 35 Shinanomachi, Shinjuku-ku, Tokyo, 160-8582, Japan

    Kenta Maruyama, Gen-ichiro Sano & Koichi Matsuo

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  1. Kenta Maruyama
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  2. Gen-ichiro Sano
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  3. Koichi Matsuo
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Correspondence to Koichi Matsuo.

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Maruyama, K., Sano, Gi. & Matsuo, K. Murine osteoblasts respond to LPS and IFN-γ similarly to macrophages. J Bone Miner Metab 24, 454–460 (2006). https://doi.org/10.1007/s00774-006-0708-x

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  • DOI: https://doi.org/10.1007/s00774-006-0708-x

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