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Gene expression of connective tissue growth factor (CTGF/CCN2) in calcifying tissues of normal and cbfa1-null mutant mice in late stage of embryonic development

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Abstract

Connective tissue growth factor (CTGF/CCN2), one of the most recently described growth factors, is produced by chondrocytes, vascular endothelial cells, and transforming growth factor (TGF)-β-stimulated fibroblasts. CTGF was isolated from a chondrosarcoma-derived chondrocytic cell line, HCS-2/8, and found to be normally expressed in cartilage tissues, especially in hypertrophic chondrocytes, and also to stimulate both the proliferation and the differentiation of chondrocytes in vitro. Therefore, CTGF is thought to be one of the most important regulators of endochondral ossification in vivo. Herein we describe the expression pattern of the ctgf gene in the calcifying tissues of normal developing mouse embryos in comparison with that in core binding factor a1 (Cbfa1)-targeted mutant (cbfa1-null) mouse embryos, in which impaired development and growth were characteristically observed in the skeletal system. After 15 days of development (E15), the expression of ctgf was detected in the zone of hypertrophy and provisional calcification, in which ossification proceeds toward the epiphysis during the skeletal development of the mouse embryo. Furthermore, ctgf was expressed in developing molar and incisal tooth germs around the perinatal stage. However, no expression of the gene was found in the cbfa1-null mouse embryos. These results indicate that CTGF may have certain important roles in the development of the calcifying tissues in the mouse embryo.

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Authors and Affiliations

  1. Department of Oral Function and Anatomy, Okayama University Graduate School of Medicine and Dentistry, Okayama, Japan

    Tomoichiro Yamaai & Tomosada Sugimoto

  2. Department of Biochemistry and Molecular Dentistry, Okayama University Graduate School of Medicine and Dentistry, 2-5-1 Shikata-cho, Okayama, 700-8525, Japan

    Tohru Nakanishi, Masahiro Asano, Kumiko Nawachi, Gen Yoshimichi, Kazumi Ohyama & Masaharu Takigawa

  3. Department of Medicine, Osaka University Medical School, Suita, Japan

    Toshifumi Komori

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  1. Tomoichiro Yamaai
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  2. Tohru Nakanishi
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  4. Kumiko Nawachi
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  9. Masaharu Takigawa
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Correspondence to Masaharu Takigawa.

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Yamaai, T., Nakanishi, T., Asano, M. et al. Gene expression of connective tissue growth factor (CTGF/CCN2) in calcifying tissues of normal and cbfa1-null mutant mice in late stage of embryonic development. J Bone Miner Metab 23, 280–288 (2005). https://doi.org/10.1007/s00774-004-0600-5

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