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Preparation and certification of a reference material for the total mercury and methylmercury mass fractions in fish

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Abstract

Certified reference materials (CRMs) play an important role in tracing results to the International System of Units through an unbroken chain of comparisons. Demand for new certified reference materials is steadily increasing in all areas. In Brazil, the demand for CRMs exceeds their availability by far, and the needs of the scientific community are not met. Food production is one area where CRMs are required, and they play an important role for export and local products. This paper describes the preparation and certification of a reference material for the content of mercury and methylmercury in fish samples. The material selection, preparation, homogeneity and stability studies, and characterization are described. Certification was performed by flow injection analysis–cold vapor atomic absorption spectrometry (FIA–CV-AAS) and isotope dilution–inductively coupled plasma mass spectrometry (ID–ICP-MS), which is a primary method. The standards ISO 30 (ABNT 30–34) and ISO Guide 35 were used as a basis for the preparation and characterization of the material. The material was certified for the mass fractions of total mercury w(total Hg) = (0.271 ± 0.059) μg g−1 and methylmercury w(MeHg) = (0.245 ± 0.053) μg g−1.

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References

  1. Voegborlo RB, Akagi H (2007) Determination of mercury in fish by cold vapour atomic absorption spectrometry using an automatic mercury analyzer. Food Chem 100:853–858

    Article  CAS  Google Scholar 

  2. Hight SC, Cheng J (2006) Determination of methylmercury and estimation of total mercury in seafood using high performance liquid chromatography (HPLC) and inductively coupled plasma-mass spectrometry (ICP-MS): method development and validation. Anal Chim Acta 567:160–172

    Article  CAS  Google Scholar 

  3. Río-Segade S, Bendicho C (1999) Determination of total and inorganic mercury in biological and environmental samples with on-line oxidation coupled to flow injection-cold vapour atomic absorption spectrometry. Spectrochim Acta Part B 54:1129–1139

    Article  Google Scholar 

  4. ISO GUIA 34 (2004) Requisitos gerais para competência de produtores de material de referência. Associação Brasileira de Normas Técnicas, Rio de Janeiro

  5. ISO Guide 35 (2006) Reference materials—general and statistical principles for certification. International Organization for Standardization, Geneva

  6. Azevedo JS, Sarkis JES, Oliveira TA, Ulrich JC (2012) Tissue-specific mercury concentrations in two catfish species from the Brazilian coast. Braz J Oceanogr 60(2):209–217

    Article  Google Scholar 

  7. Akagi H, Malm O, Branches FJP, Kinjo Y, Kashima Y, Guimaraes JRD, Oliveira RB, Haraguchi K, Pfeiffer WC, Takizawa Y, Kato H (1995) Human exposure to mercury due to gold mining in the Tapajos river basin, Amazon, Brazil: speciation of mercury in human hair, blood and urine. Water Air Soil Pollut 80:85–94

    Article  CAS  Google Scholar 

  8. Hortellani MA, Sarkis JES, Bonetti J, Bonetti C (2005) Evaluation of mercury contamination in sediments from Santos—São Vicente Estuarine System, São Paulo State, Brazil. J Braz Chem Soc 16(6A):1140–1149

    Google Scholar 

  9. Accredited testing and calibration laboratories (RBLE and RBC). http://www.inmetro.gov.br/laboratorios/rble/docs/CRL0242.pdf

  10. Horvat M, May K, Stoeppler M, Byrne AR (1988) Comparative studies of methylmercury determination in biological and environmental samples. Appl Organomet Chem 2:515–524

    Article  CAS  Google Scholar 

  11. May K, Stoeppler M, Reisinger K (1988) Studies in the ratio total mercury methylmercury in the aquatic food chain. In: Merian E, Frei RW, Hardi W, Schlatter CH (ed) Carcinogenic and mutagenic metal compounds 2. Gordon and Breach Science Publishers, New York

  12. Quevauviller P, Drabaek I, Muntau H, Bianchi M, Bortoli A, Griepink B (1996) Certified reference materials (CRMs 463 and 464) for the quality control of total and methyl mercury determination in tuna fish. Trends Anal Chem 15:160–167

    Article  CAS  Google Scholar 

  13. Garcia Alonso JI (1995) Determination of fission products and actinides by inductively coupled plasma-mass spectrometry using isotope dilution analysis: a study of random and systematic errors. Anal Chim Acta 312:57–78

    Article  Google Scholar 

  14. Barwick VJ, Ellison SLR (1999) Measurement uncertainty: approaches to the evaluation of uncertainties associated with recovery. Analyst 124:981–990

    Article  CAS  Google Scholar 

  15. Ellison SLR, Williams A (eds) (2012) Eurachem/CITAC guide: quantifying uncertainty in analytical measurement, 3rd edn. ISBN 978-0-948926-30-3. Available from http://www.eurachem.org

  16. JCGM 100 (2008) Evaluation of measurement data—guide to the expression of uncertainty in measurement. Joint Committee for Guides in Metrology (JCGM)

  17. Fearn T, Thompson M (2001) A new test for “sufficient homogeneity”. Analyst 126:1414–1417

    Article  CAS  Google Scholar 

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Acknowledgments

The authors are grateful to the IPEN, Conselho Nacional de Desenvolvimento Científico e Tecnológico—CNPQ, Fundação de Apoio ao Instituto de Pesquisas Tecnológicas—FIPT, and Ecomar Industria de Pesca and would like to thank Dr. Vera Ponçano (Rede Metrológica do Estado de São Paulo); Marcos Hortellani Msc, Helena Miho Msc, and Dr. Vera Maihara (IPEN); and Dilza Mantovani Msc (Instituto Tecnológico de Alimentos—ITAL).

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Authors and Affiliations

  1. Centro de Química e Meio Ambiente, Instituto de Pesquisas Energéticas e Nucleares (IPEN), São Paulo, Brazil

    J. C. Ulrich & J. E. S. Sarkis

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  1. J. C. Ulrich
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  2. J. E. S. Sarkis
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Correspondence to J. C. Ulrich.

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Ulrich, J.C., Sarkis, J.E.S. Preparation and certification of a reference material for the total mercury and methylmercury mass fractions in fish. Accred Qual Assur 18, 511–516 (2013). https://doi.org/10.1007/s00769-013-1019-2

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  • DOI: https://doi.org/10.1007/s00769-013-1019-2

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