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Quantification of piperine in different varieties of Piper nigrum by a validated high-performance thin-layer chromatography‒densitometry method

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Abstract

Piperine is the most important alkaloid molecule present in Piper nigrum. In this study, a simple, rapid, quantitative high-performance thin-layer chromatography (HPTLC) technique proposed by International Organization for Standardization (ISO) was optimized and validated for the quantitative estimation of piperine in P. nigrum. Densitometric analysis of piperine was carried out in the absorbance mode at 254 nm. The method gave spot at RF = 0.63, corresponding to piperine in different samples. The limit of detection and limit of quantification per spot were confirmed with the mobile phase toluene‒ethyl acetate (3:2, V/V). Linear regression analysis data for the calibration plot for piperine showed a good linear relationship with a correlation coefficient (r2) of 0.99077 in the concentration range of 200‒1000 ng per spot. The method was validated for sensitivity, linearity, accuracy, precision and specificity as per the International Council for Harmonisation guidelines. This simple, precise and accurate HPTLC method provides a novel approach for the routine analysis of black pepper for quality control and standardization purposes, which may be further useful for industrial perspectives.

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Abbreviations

ICH:

International Council for Harmonisation

r :

Correlation coefficient

R F :

Retardation factor

HPTLC:

High-performance thin-layer chromatography

ISO:

International Organization for Standardization

LOD:

Limit of detection

LOQ:

Limit of quantification

RSD:

Relative standard deviation

LQC:

Low quality control

MQC:

Medium quality control

HQC:

High quality control

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Acknowledgements

The authors are thankful to the National Medicinal Plant Board, Grant Number: F.No.Z.18017/187/CSS/R&D/WB-02/2017-18-NMPB-IV A, Ministry of AYUSH for financial support. The authors are also thankful to the Institute of Bioresources and Sustainable Development, an autonomous institute under Dept. of Biotechnology (DBT), Govt. of India, Imphal, India, for necessary help and support through IBSD-JU joint collaboration.

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Authors and Affiliations

  1. Department of Pharmaceutical Technology, School of Natural Product Studies, Jadavpur University, Kolkata, 700032, India

    Shibu Narayan Jana, Subhadip Banerjee, Pallab Kanti Haldar, Barun Dasgupta & Pulok K. Mukherjee

  2. Department of Instrumentation and Electronics Engineering, Jadavpur University, Salt Lake Campus, Kolkata, 700106, India

    Dilip Sing & Rajib Bandyopadhayay

  3. Department of Biotechnology, Institute of Bioresources and Sustainable Development, Govt. of India, Takyelpat, Imphal, Manipur, 795001, India

    Amit Kar, Nanaocha Sharma & Pulok K. Mukherjee

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  1. Shibu Narayan Jana
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Correspondence to Pulok K. Mukherjee.

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Jana, S.N., Sing, D., Banerjee, S. et al. Quantification of piperine in different varieties of Piper nigrum by a validated high-performance thin-layer chromatography‒densitometry method. JPC-J Planar Chromat 34, 521–530 (2021). https://doi.org/10.1007/s00764-021-00149-x

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