Abstract
Currently, little is known about the function of l-arginine in the homeostasis of intestinal lipid metabolism. This study was conducted to test the hypothesis that dietary l-arginine supplementation may alter intestinal microbiota and lipid metabolism in tilapia. Tilapia were fed a basal diet (containing 16.9 g l-arginine per kilogram diets) or the basal diet supplemented with 1% or 2% l-arginine for 8 wks. In the present study, we found that dietary supplementation with 1% or 2% l-arginine induced a shift in the community structure of gut microbiota, as showed by increased (p < 0.05) α-diversity, altered (p < 0.05) β-diversity and function profile. This finding coincided with decreased lipid accretion in the intestine of tilapia, which was associated with an enhancement in mRNA levels for peroxisome proliferator-activated receptor α (Pparα), acyl-coenzyme a oxidase 1 (Acox1), and peroxisome proliferator-activated receptor γ coactivator-1α (Pgc-1α). Using intestinal epithelial cell culture, we demonstrated that the lipid-lowering effect of l-arginine was mainly mediated by activating the AMP-activated protein kinase (AMPK) signaling pathway, carnitine palmitoyltransferase 1 (CPT1), and PPARα, as well as mRNA levels for Acox1 and Acox2. Collectively, our results suggest that dietary l-arginine supplementation of tilapia changed the intestinal microbiota and activated intestinal fatty acid oxidation. However, future studies are warranted to determine the relationship between microbiota and lipid metabolism in the intestine.
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Abbreviations
- ACC:
-
Acetyl-CoA carboxylase
- Acox1 :
-
Acyl-coenzyme a oxidase 1
- Acox2 :
-
Acyl-coenzyme a oxidase 2
- AMPK:
-
AMP-activated protein kinase
- Apob100 :
-
Apolipoprotein B100
- Arg:
-
l-Arginine
- Atgl :
-
Adipose triglyceride lipase
- Cd36 :
-
Cluster determinant 36
- Cpt1:
-
Carnitine palmitoyltransferase 1
- Ctrl:
-
Control
- Dgat2 :
-
Diacylglycerol O-acyltransferase 2
- Fasn :
-
Fatty acid synthase
- FBS:
-
Fetal bovine serum
- Hsl :
-
Hormone-sensitive lipase
- OA:
-
Oleic acid
- ORO:
-
Oil red O
- Mgl :
-
Monoacylglycerol lipase
- Pgc-1α :
-
Peroxisome proliferator-activated receptor γ coactivator-1α
- Pparα :
-
Peroxisome proliferator-activated receptor α
- Srebf-1 :
-
Sterol regulatory element-binding transcription factor 1
- TGs:
-
Triglycerides
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This work was supported by the Grants from the National Natural Science Foundation of China (No. 31625025, 31572410, 31572412, 31272450, 31272451) and the “111” Project (B16044).
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The authors’ responsibilities were as follows. ZW designed research; SL conducted the research; CW and ZW analyzed the data; ZW and SL wrote the paper. ZW had responsibility for the final content. All authors read and approved the final manuscript.
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Li, S., Wang, C. & Wu, Z. Dietary l-arginine supplementation of tilapia (Oreochromis niloticus) alters the microbial population and activates intestinal fatty acid oxidation. Amino Acids 54, 339–351 (2022). https://doi.org/10.1007/s00726-021-03018-3
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DOI: https://doi.org/10.1007/s00726-021-03018-3