Skip to main content
SpringerLink
Log in

Metabolomic study of urinary polyamines in rat exposed to 915 MHz radiofrequency identification signal

  • Original Article
  • Published:
Amino Acids Aims and scope Submit manuscript

Abstract

Metabolomic analysis of urinary polyamines (PAs) from rat exposed to 915 MHz radiofrequency identification (RFID) signal for 8 h/day for 2 weeks was performed by gas chromatography–mass spectrometry as N-ethoxycarbonyl/N-pentafluoropropionyl derivatives. Large alterations in nine PA levels including four aliphatic and five acetylated PAs were monitored in sham-exposed and RFID-exposed groups. Total PA and urinary levels of N 1-acetylputrescine, N 1-acetylcadaverine, putrescine, cadaverine, N 1-acetylspermidine, N 8-acetylspermidine, spermidine and spermine were reduced, whereas N 1-acetylspermine was significantly increased after sham and RFID exposure compared with those before exposure. Their levels were normalized to the corresponding group means before exposure and then plotted into star symbol patterns. N 1-Acetylspermine after RFID exposure was 54 % higher compared to the level before RFID exposure, while it was elevated by only 17 % in the sham group. The results suggest that 915 MHz RFID exposure may induce metabolic disturbance of PA. It may also elevate spermidine/spermine acetyltransferase (SSAT) activity. Thus, the present metabolic profiling combined with star pattern recognition method might be useful for understanding the complexity of biochemical events after exposure to RFID signal.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

Similar content being viewed by others

Abbreviations

PAs:

Polyamines

RF-EMF:

Radiofrequency electromagnetic field

RFID:

Radiofrequency identification

N-EOC-N-PFP:

N-ethoxycarbonyl-N-pentafluoropropionyl

GC–MS:

Gas chromatography-mass spectrometry

SIM:

Selected ion monitoring

ECF:

Ethyl chloroformate

IS:

Internal standard

PFPA:

Pentafluoropropionyl anhydride

SSAT:

Spermidine/spermine acetyltransferase

References

  • Anthony EP (2008) Spermidine/spermine-N 1-acetyltransferase: a key metabolic regulator. Am J Physiol Endocrinol Metab 294:E995–E1010

    Article  Google Scholar 

  • Billaudel B, Taxile M, Poulletier de Gannes F, Ruffie G, Lagroye I, Veyret B (2009a) Effects of exposure to DAMPS and GSM signals on ornithine decarboxylase (ODC) activity: II. SH-SY5Y human neuroblastoma cells. Int J Radiat Biol 85(6):519–522. doi:10.1080/09553000902883778

  • Billaudel B, Taxile M, Ruffie G, Veyret B, Lagroye I (2009b) Effects of exposure to DAMPS and GSM signals on ornithine decarboxylase (ODC) activity: I. L-929 mouse fibroblasts. Int J Radiat Biol 85(6):510–518. doi:10.1080/09553000902883760

    Article  PubMed  CAS  Google Scholar 

  • Brainard GC, Kavet R, Kheifets LI (1999) The relationship between electromagnetic field and light exposures to melatonin and breast cancer risk: a review of the relevant literature. J Pineal Res 26:65–100

    Article  PubMed  CAS  Google Scholar 

  • Hoyto A, Juutilainen J, Naarala J (2007) Ornithine decarboxylase activity of L929 cells after exposure to continuous wave or 50 Hz modulated radiofrequency radiation a replication study. Bioelectromagnetics 28:501–508

    Article  PubMed  CAS  Google Scholar 

  • ICNIRP (1998) Guidelines for limiting exposure to time-varying electric magenetic, and electromagnetic fields (up to 300 GHz). Health Phys 74:494–522

    Google Scholar 

  • Jell J, Merali S, Hensen ML, Mazurchuk R, Spernyak JA, Diegelman P, Kisiel ND, Barrero C, Deeb KK, Alhonen L, Patel MS, Porter CW (2007) Genetically altered expression of spermidine/spermine N 1-acetyltransferase affects fat metabolism in mice via acetyl-CoA. J Biol Chem 282(11):8404–8413

    Article  PubMed  CAS  Google Scholar 

  • Jung KB, Kim TH, Kim JL, Doh HJ, Chung YC, Choi JH, Pack JK (2008) Development and validation of reverberation-chamber type whole-body exposure system for mobile-phone frequency. Electromagn Biol Med 27(1):73–82. doi:10.1080/15368370701878895

    Article  PubMed  CAS  Google Scholar 

  • Kim HS, An YS, Paik MJ, Lee YS, Choi HD, Kim BC, Pack JK, Kim N, Ahn YH (2013a) The effects of exposure to 915 MHz radiofrequency identification on cerebral glucose metabolism in rat: a [F-18] FDG micro-Pet Study. Int J Radiat Biol 89(9):750–755. doi:10.3109/09553002.2013.791756

    Article  PubMed  CAS  Google Scholar 

  • Kim HS, Paik MJ, Kim YJ, Lee G, Lee YS, Choi HD, Kim BC, Pack JK, Kim N, Ahn YH (2013b) Effects of whole-body exposure to 915 MHz RFID on secretory functions of the thyroid system in rats. Bioelectromagnetics 34(7):521–529. doi:10.1002/bem.21797

    PubMed  CAS  Google Scholar 

  • Kim HS, Kim YJ, Lee YH, Lee YS, Choi HD, Pack JK, Kim N, Ahn YH (2015) Effect of whole-body exposure to the 848.5-MHz code division multiple access (CDMA) electromagnetic field on adult neurogenesis in the young, healthy rat brain. Int J Radiat Biol 91:354–359

    Article  PubMed  CAS  Google Scholar 

  • Monti MG, Ghiaroni S, Marverti G, Montanari M, Moruzzi MS (2004) Polyamine depletion switches the form of 2-deoxy-d-riboseinduced cell death from apoptosis to necrosis in HL-60 cells. Int J Biochem Cell Biol 36:1238–1248

    Article  PubMed  CAS  Google Scholar 

  • Paik MJ, Lee SM, Cho KH, Kim KR (2006) Urinary polyamines and N-acetylated polyamines in four patients with Alzheimer’s disease as their N-ethoxycarbonyl-N-pentafluoropropionyl derivatives by gas chromatography-mass spectrometry in selected ion monitoring mode. Anal Chim Acta 576:55–60. doi:10.1016/j.aca.2006.01.070

    Article  PubMed  CAS  Google Scholar 

  • Paik MJ, Lee KA, Jeong R, Kim KR, Park CS, Ahn YH, Lee G (2007) Pattern recognition analysis of polyamines in the plasma of rat models with adenovirus infection. Clin Chim Acta 380(1–2):228–231. doi:10.1016/j.cca.2007.02.003

    Article  PubMed  CAS  Google Scholar 

  • Paik MJ, Kwon D, Cho J, Kim KR (2009) Altered urinary polyamine patterns of cancer patients under acupuncture therapy. Amino Acids 37:407–413. doi:10.1007/s00726-008-0169-8

    Article  PubMed  CAS  Google Scholar 

  • Paik MJ, Ahn YH, Lee PH, Kang H, Park CB, Choi S, Lee G (2010) Polyamine patterns in the cerebrospinal fluid of patients with Parkinson’s disease and multiple system atrophy. Clin Chim Acta 411:1532–1535. doi:10.1016/j.cca.2010.05.034

    Article  PubMed  CAS  Google Scholar 

  • Pegg AE (2008) Spermidine/Spermine-N(1)-Acetyltransferase: a key metabolic regulator. Am J Physiol Endocrinol Metab 294(6):E995–E1010. doi:10.1152/ajpendo.90217

    Article  PubMed  CAS  Google Scholar 

  • Takao K, Rickhag M, Hegardt C, Oredsson S, Persson L (2006) Induction of apoptotic cell death by putrescine. Int J Biochem Cell Biol 38:621–6288

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

This work was supported by the IT R&D program of MSIP/IITP (B0138-15-1002, study on the EMF exposure control in smart society) and by Suncheon Research Center for Natural Medicines.

Author information

Authors and Affiliations

  1. College of Pharmacy, Sunchon National University, Sunchon, 540-950, Republic of Korea

    Man-Jeong Paik

  2. Department of Neurosurgery, Ajou University School of Medicine, Suwon, 443-749, Republic of Korea

    Hye Sun Kim & Young Hwan Ahn

  3. Neuroscience Graduate Program, Department of Biomedical Sciences, Graduate School of Ajou University, Suwon, Republic of Korea

    Young Hwan Ahn

  4. Division of Life Science and Pharaceuticals, College of Pharmacy, Ewha Woman’s University, Seoul, Republic of Korea

    Yun-Sil Lee

  5. Radio Technology Research Department, Electronics and Telecommunications Research Institute, Daejeon, Republic of Korea

    Hyung Do Choi

  6. Department of Radio Sciences and Engineering, College of Engineering, Chungnam National University, Daejeon, Republic of Korea

    Jeong-Ki Pack

  7. School of Electrical and Computer Engineering, Chungbuk National University, Cheongju, Republic of Korea

    Nam Kim

Authors
  1. Man-Jeong Paik
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Hye Sun Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Yun-Sil Lee
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Hyung Do Choi
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Jeong-Ki Pack
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Nam Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Young Hwan Ahn
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Man-Jeong Paik or Young Hwan Ahn.

Ethics declarations

Conflict of interest

The authors declare that they have no competing financial interests.

Additional information

Handling Editor: S. Beninati.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Paik, MJ., Kim, H.S., Lee, YS. et al. Metabolomic study of urinary polyamines in rat exposed to 915 MHz radiofrequency identification signal. Amino Acids 48, 213–217 (2016). https://doi.org/10.1007/s00726-015-2079-x

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00726-015-2079-x

Keywords

Search

Navigation