Skip to main content
SpringerLink
Log in

Fast analysis of wine for total homocysteine content by high-performance liquid chromatography

  • Original Article
  • Published:
Amino Acids Aims and scope Submit manuscript

Abstract

Alimentary methionine is believed to be the main source for plasma homocysteine. Recent literature supplies information about homocysteine content in daily food components, but not in wine, an attractive complement of the evening meal in some western countries. In this communication, a simple and fast high-performance liquid chromatography method for determination of total homocysteine in wine is described. The two steps procedure relies on reduction of the disulfide forms of homocysteine with tris-(2-carboxyethyl)phosphine and on-column derivatization with o-phthaldialdehyde followed by separation and fluorescence detection. The entire analysis time, including sample work-up, amounts 14 min. The calibration performed with wine matrix, spiked with homocystine within the practical concentration range, proved linear response of the detector. The proposed method was applied for the analysis of 32 different types of wines for total homocysteine. The average concentration of the analyte was 10.31 (±4.25) µM and 6.11 (±3.44) µM for red (n = 23) and white (n = 9) wines, respectively.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

Abbreviations

Hcy:

Homocysteine.

tHcy:

Total homocysteine

(Hcy)2 :

Homocystine

TCEP:

tris-(2-Carboxyethyl)phosphine

OPA:

o-Phthaldialdehyde

LOQ:

Limit of quantitation

LOD:

Limit of detection

RSD:

Relative standard deviation

References

  • Ayaori M, Hisada T, Yoshida H, Shige H, Ito T, Nakajima K, Higashi K, Yonemura A, Ishikawa T, Ohsuzu F, Saionji K, Tamai S, Nakamura H (2000) Effect of alcohol intake on the levels of plasma homocysteine in healthy males. J Nutr Sci Vitaminol 46:171–174

    Article  PubMed  CAS  Google Scholar 

  • Bald E, Głowacki R (2005) Analysis of saliva for glutathione and metabolically related thiols by liquid chromatography with ultraviolet detection. Amino Acids 28:431–433

    Article  PubMed  CAS  Google Scholar 

  • Bleich S, Bleich K, Kropp S, Bittermann HJ, Degner D, Sperling W, Ruther E, Kornhuber J (2001) Moderate alcohol consumption in social drinkers raises plasma homocysteine levels: a contradiction to the ‘French Paradox’? Alcohol 36:189–192

    Article  CAS  Google Scholar 

  • Burger M, Mensink G, Bronstrup A, Thierfelder W, Pietrzik K (2004) Alcohol consumption and its relation to cardiovascular risk factors in Germany. Eur J Clin Nutr 58:605–614

    Article  PubMed  CAS  Google Scholar 

  • De Bree A, Verschuren WMM, Blom HJ, Kromhout D (2001) Lifestyle factors and plasma homocysteine concentrations in a general population sample. Am J Epidemiol 154:150–154

    Article  PubMed  Google Scholar 

  • Demirkol O, Adams C, Ercal N (2004) Biologically important thiols in various vegetables and fruits. J Agric Food Chem 52:8151–8154

    Article  PubMed  CAS  Google Scholar 

  • Ducros V, Demuth K, Sauvant MP, Quillard M, Caussé E, Candito M, Read MH, Gerhardt MF (2002) Methods for homocysteine analysis and biological relevance of the results. J Chromatogr B 781:207–226

    Article  CAS  Google Scholar 

  • Eikelboom JW, Lonn E, Genest J, Hankey G, Yusuf S (1999) Homocyst(e)ine and cardiovascular disease: a critical review of the epidemiologic evidence. Ann Intern Med 131:363–375

    PubMed  CAS  Google Scholar 

  • FDA Guidance for Industry Bioanalytical Method Validation (2001) http://www.fda.gov/cder/guidance/4252fnl.pdf

  • Fonseca V, Guba SC, Fink LM (1999) Hyperhomocysteinemia and the endocrine system: implications for atherosclerosis and thrombosis. Endocrinol Rev 20:738–759

    Article  CAS  Google Scholar 

  • Głowacki R, Borowczyk K, Bald E, Jakubowski H (2010a) On-column derivatization with o-phthaldialdehyde for fast determination of homocysteine in human urine. Anal Bioanal Chem. doi:10.1007/s00216-010-3456-7 (in press)

  • Głowacki R, Bald E, Jakubowski H (2010b) An on-column derivatization method for the determination of homocysteine-thiolactone and protein N-linked homocysteine. Amino Acids (in press)

  • Husemoen LLN, Linneberg A, Fenger M, Thuesen BH, Jørgensen T (2009) Changes in lifestyle, biological risk factors and total homocysteine in relation to MTHFR C677T genotype: a 5-year follow-up study. Eur J Clin Nutr 63:1233–1240

    Article  PubMed  CAS  Google Scholar 

  • Jacobsen DW (1993) Cardiovascular disorders (risk assessment). Anal Chem 65:367–373

    Article  Google Scholar 

  • Jacques PF, Bostom AG, Wilson PWF, Rich S, Rosenberg IH, Selhub J (2001) Determinants of plasma total homocysteine concentration in the Framingham offspring cohort. Am J Clin Nutr 73:613–621

    PubMed  CAS  Google Scholar 

  • Pexa A, Fisher K, Deussen A, Henke T (2008) Homocysteine in food. Eur Food Res Technol 226:933–935

    Article  CAS  Google Scholar 

  • Rajdl D, Racek J, Trefil L, Siala K (2007) Effect of white wine consumption on oxidative stress markers and homocysteine levels. Physiol Res 56:203–212

    PubMed  CAS  Google Scholar 

  • Refsum H, Ueland PM, Nygard O, Vollet SE (1998) Homocysteine and cardiovascular disease. Ann Rev Med 49:31–62

    Article  PubMed  CAS  Google Scholar 

  • Sakamoto A, Nishimura Y, Ono H, Sakura N (2002) Betaine and homocysteine concentrations in foods. Pediatr Int 44:409–413

    Article  PubMed  CAS  Google Scholar 

  • Sakuta H, Suzuki T (2005) Alcohol consumption and plasma homocysteine. Alcohol 37:73–77

    Article  PubMed  CAS  Google Scholar 

  • Ubbink JB, Fehily AM, Pickering J, Elwood PC, Vermaak HWJ (1998) Homocysteine and ischaemic heart disease in the Caerphilly cohort. Atherosclerosis 140:349–356

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

The authors are grateful to University of Łódź for the financial support of this research.

Author information

Authors and Affiliations

  1. Department of Environmental Chemistry, University of Łódź, Lodz, Poland

    Rafał Głowacki, Kamila Borowczyk & Edward Bald

Authors
  1. Rafał Głowacki
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Kamila Borowczyk
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Edward Bald
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Edward Bald.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Głowacki, R., Borowczyk, K. & Bald, E. Fast analysis of wine for total homocysteine content by high-performance liquid chromatography. Amino Acids 42, 247–251 (2012). https://doi.org/10.1007/s00726-010-0509-3

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00726-010-0509-3

Keywords

Search

Navigation