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The role of heme oxygenase-1 in down regulation of PGE2 production by taurine chloramine and taurine bromamine in J774.2 macrophages

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Summary.

Taurine chloramine (TauCl) and taurine bromamine (TauBr), products of myeloperoxidase halide system, exert anti-inflammatory properties. TauCl was demonstrated to inhibit the production of a variety of pro-inflammatory mediators including cyclooxygenase-2 (COX-2) dependent production of prostaglandin E2 (PGE2). Recently we have demonstrated that both major leukocyte haloamines, TauCl and TauBr, induced expression of HO-1 in non-activated and LPS-activated J774.2 macrophages. In this study, we have shown that TauCl and TauBr, at non-cytotoxic concentrations, inhibited the production of (PGE2) without altering the expression of COX-2 protein, in LPS/IFN-γ stimulated J774.2 cells. The inhibitory effect of TauCl and TauBr was reversed by chromium III mesoporhyrin (CrMP), an inhibitor of HO-1 activity. Our data suggest that HO-1 might participate in anti-inflammatory effects of TauCl/TauBr possibly by inhibition of COX-2 activity and decrease of PGE2 production.

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Abbreviations

COX-2:

Cyclooxygenase-2

DMSO:

dimethyl sulfoxide

MTT:

3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide

FCS:

fetal calf serum

HO-1:

heme oxygenase-1

IFNγ:

interferon γ

IL-6:

interleukin-6

IL-8:

interleukin-8

IL-10:

interleukin-10

IL-12:

interleukin-12

iNOS:

inducible nitric oxide synthase

LPS:

lipopolysaccharide

PBS:

phosphate buffered saline

PD 98059:

2′-amino-3′-methoxyflavone

PGE2 :

prostaglandin E2

PGJ2 :

prostaglandin J2

ROS:

reactive oxygen species

RT:

room temperature

SB 203580:

4-(4-fluorophenyl)-2-(4-methylsulfinylphenyl)-5-(4-pyridyl)1H-imidazole-

SDS:

dodecyl sodium sulfate

TauBr:

taurine bromamine

TauCl:

taurine chloramine

TGF-β:

tumor growth factor-β

TNF-α:

tumor necrosis factor

References

  • NG Abraham (2003) ArticleTitleTherapeutic applications of human heme oxygenase gene transfer and gene therapy Curr Pharm Des 9 2513–2524 Occurrence Handle14529550 Occurrence Handle10.2174/1381612033453758 Occurrence Handle1:CAS:528:DC%2BD3sXotlWjtro%3D

    Article  PubMed  CAS  Google Scholar 

  • IC Chen KC Huang WW Lin (2006) ArticleTitleHMG-CoA reductase inhibitors upregulate heme oxygenase-1 expression in murine RAW264.7 macrophages via ERK, P38 MAPK and protein kinase G pathways Cell Signal 18 32–39 Occurrence Handle16214041 Occurrence Handle10.1016/j.cellsig.2005.03.016 Occurrence Handle1:CAS:528:DC%2BD2MXhtVOksr7K

    Article  PubMed  CAS  Google Scholar 

  • P Gong AI Cederbaum N Nieto (2003) ArticleTitleIncreased expression of cytochrome P450 2E1 induces heme oxygenase-1 through ERK MAPK pathway J Biol Chem 278 29693–29700 Occurrence Handle12777398 Occurrence Handle10.1074/jbc.M304728200 Occurrence Handle1:CAS:528:DC%2BD3sXlvFOns7o%3D

    Article  PubMed  CAS  Google Scholar 

  • A Haider R Olszanecki R Gryglewski ML Schwartzman E Lianos A Kappas A Nasjletti NG Abraham (2002) ArticleTitleRegulation of cyclooxygenase by the heme–heme oxygenase system in microvessel endothelial cells J Pharmacol Exp Ther 300 188–194 Occurrence Handle11752115 Occurrence Handle10.1124/jpet.300.1.188 Occurrence Handle1:CAS:528:DC%2BD38Xht1Wmsw%3D%3D

    Article  PubMed  CAS  Google Scholar 

  • E Kontny W Rudnicka J Kowalczewski J Marcinkiewicz W Maslinski (2003) ArticleTitleSelective inhibition of cyclooxygenase 2-generated prostaglandin E2 synthesis in rheumatoid arthritis synoviocytes by taurine chloramine Arthritis Rheum 48 1551–1555 Occurrence Handle12794822 Occurrence Handle10.1002/art.10975 Occurrence Handle1:CAS:528:DC%2BD3sXltlSgur0%3D

    Article  PubMed  CAS  Google Scholar 

  • E Kontny K Szczepanska J Kowalczewski M Kurowska I Janicka J Marcinkiewicz W Maslinski (2000) ArticleTitleThe mechanism of taurine chloramine inhibition of cytokine (interleukin-6, interleukin-8) production by rheumatoid arthritis fibroblast-like synoviocytes Arthritis Rheum 43 2169–2177 Occurrence Handle11037876 Occurrence Handle10.1002/1529-0131(200010)43:10<2169::AID-ANR4>3.0.CO;2-# Occurrence Handle1:CAS:528:DC%2BD3cXnslWjsrY%3D

    Article  PubMed  CAS  Google Scholar 

  • J Marcinkiewicz A Grabowska J Bereta T Stelmaszynska (1995) ArticleTitleTaurine chloramine, a product of activated neutrophils, inhibits in vitro the generation of nitric oxide and other macrophage inflammatory mediators J Leukoc Biol 58 667–674 Occurrence Handle7499964 Occurrence Handle1:CAS:528:DyaK2MXhtVSitr%2FJ

    PubMed  CAS  Google Scholar 

  • J Marcinkiewicz B Chain B Nowak A Grabowska K Bryniarski J Baran (2000) ArticleTitleAntimicrobial and cytotoxic activity of hypochlorous acid: interactions with taurine and nitrite Inflamm Res 49 280–289 Occurrence Handle10939618 Occurrence Handle10.1007/PL00000208 Occurrence Handle1:CAS:528:DC%2BD3cXlsVCjtr0%3D

    Article  PubMed  CAS  Google Scholar 

  • J Marcinkiewicz M Mak M Bobek R Biedron A Bialecka M Koprowski E Kontny W Maslinski (2005) ArticleTitleIs there a role of taurine bromamine in inflammation? Interactive effects with nitrite and hydrogen peroxide Inflamm Res 54 42–49 Occurrence Handle15723204 Occurrence Handle10.1007/s00011-004-1322-9 Occurrence Handle1:CAS:528:DC%2BD2MXkvVWrtrg%3D

    Article  PubMed  CAS  Google Scholar 

  • J Marcinkiewicz M Kurnyta R Biedron M Bobek E Kontny W Maslinski (2006) ArticleTitleAnti-inflammatory effects of taurine derivatives (taurine chloramine, taurine bromamine, and taurolidine) are mediated by different mechanisms Adv Exp Med Biol 583 481–492 Occurrence Handle17153635 Occurrence Handle10.1007/978-0-387-33504-9_54 Occurrence Handle1:CAS:528:DC%2BD1MXhtFWis7Y%3D

    Article  PubMed  CAS  Google Scholar 

  • RG Midwinter AV Peskin MC Vissers CC Winterbourn (2004) ArticleTitleExtracellular oxidation by taurine chloramine activates ERK via the epidermal growth factor receptor J Biol Chem 279 32205–32211 Occurrence Handle15166244 Occurrence Handle10.1074/jbc.M402070200 Occurrence Handle1:CAS:528:DC%2BD2cXmtVChsro%3D

    Article  PubMed  CAS  Google Scholar 

  • M Nagl MW Hess K Pfaller P Hengster W Gottardi (2000) ArticleTitleBactericidal activity of micromolar N-chlorotaurine: evidence for its antimicrobial function in the human defense system Antimicrob Agents Chemother 44 2507–2513 Occurrence Handle10952603 Occurrence Handle10.1128/AAC.44.9.2507-2513.2000 Occurrence Handle1:CAS:528:DC%2BD3cXmtFyltL4%3D

    Article  PubMed  CAS  Google Scholar 

  • R Olszanecki A Gebska VI Kozlovski RJ Gryglewski (2002) ArticleTitleFlavonoids and nitric oxide synthase J Physiol Pharmacol 53 571–584 Occurrence Handle12512693 Occurrence Handle1:CAS:528:DC%2BD3sXltFGjtQ%3D%3D

    PubMed  CAS  Google Scholar 

  • R Olszanecki J Marcinkiewicz (2004) ArticleTitleTaurine chloramine and taurine bromamine induce heme oxygenase-1 in resting and LPS-stimulated J774.2 macrophages Amino Acids 27 29–35 Occurrence Handle15309569 Occurrence Handle10.1007/s00726-004-0097-1 Occurrence Handle1:CAS:528:DC%2BD2cXmsVKktL8%3D

    Article  PubMed  CAS  Google Scholar 

  • E Park G Schuller-Levis JH Jia MR Quinn (1997) ArticleTitlePreactivation exposure of RAW 264.7 cells to taurine chloramine attenuates subsequent production of nitric oxide and expression of iNOS mRNA J Leukoc Biol 61 161–166 Occurrence Handle9021921 Occurrence Handle1:CAS:528:DyaK2sXhtVOrsbs%3D

    PubMed  CAS  Google Scholar 

  • MR Quinn E Park G Schuller-Levis (1996) ArticleTitleTaurine chloramine inhibits prostaglandin E2 production in activated RAW 264.7 cells by post-transcriptional effects on inducible cyclooxygenase expression Immunol Lett 50 185–188 Occurrence Handle8803618 Occurrence Handle10.1016/0165-2478(96)02542-4 Occurrence Handle1:CAS:528:DyaK28Xkt1Shtrs%3D

    Article  PubMed  CAS  Google Scholar 

  • SW Ryter LE Otterbein D Morse AM Choi (2002) ArticleTitleHeme oxygenase/carbon monoxide signaling pathways: regulation and functional significance Mol Cell Biochem 234–235 249–263 Occurrence Handle12162441 Occurrence Handle10.1023/A:1015957026924

    Article  PubMed  Google Scholar 

  • SW Ryter J Alam AM Choi (2006) ArticleTitleHeme oxygenase-1/carbon monoxide: from basic science to therapeutic applications Physiol Rev 86 583–650 Occurrence Handle16601269 Occurrence Handle10.1152/physrev.00011.2005 Occurrence Handle1:CAS:528:DC%2BD28Xktlylu7k%3D

    Article  PubMed  CAS  Google Scholar 

  • TA Swierkosz JA Mitchell TD Warner RM Botting JR Vane (1995) ArticleTitleCo-induction of nitric oxide synthase and cyclo-oxygenase: interactions between nitric oxide and prostanoids Br J Pharmacol 114 1335–1342 Occurrence Handle7541688 Occurrence Handle1:CAS:528:DyaK2MXkvFChtb8%3D

    PubMed  CAS  Google Scholar 

  • WF Stenson MW Nickells JP Atkinson (1981) ArticleTitleMetabolism of exogenous arachidonic acid by murine macrophage-like tumor cell lines Prostaglandins 21 675–689 Occurrence Handle6803301 Occurrence Handle10.1016/0090-6980(81)90225-2 Occurrence Handle1:CAS:528:DyaL3MXltlSisLs%3D

    Article  PubMed  CAS  Google Scholar 

  • EL Thomas PM Bozeman MM Jefferson CC King (1995) ArticleTitleOxidation of bromide by the human leukocyte enzymes myeloperoxidase and eosinophil peroxidase. Formation of bromamines J Biol Chem 270 2906–2913 Occurrence Handle7852368 Occurrence Handle10.1074/jbc.270.7.2906 Occurrence Handle1:CAS:528:DyaK2MXjslahtrg%3D

    Article  PubMed  CAS  Google Scholar 

  • M Vairano C Dello Russo G Pozzoli G Tringali P Preziosi P Navarra (2001) ArticleTitleA functional link between heme oxygenase and cyclo-oxygenase activities in cortical rat astrocytes Biochem Pharmacol 61 437–441 Occurrence Handle11226377 Occurrence Handle10.1016/S0006-2952(00)00582-7 Occurrence Handle1:CAS:528:DC%2BD3MXhsVOiu7Y%3D

    Article  PubMed  CAS  Google Scholar 

  • FA Wagener HD Volk D Willis NG Abraham MP Soares GJ Adema CG Figdor (2003) ArticleTitleDifferent faces of the heme-heme oxygenase system in inflammation Pharmacol Rev 55 551–571 Occurrence Handle12869663 Occurrence Handle10.1124/pr.55.3.5 Occurrence Handle1:CAS:528:DC%2BD3sXnsVynurk%3D

    Article  PubMed  CAS  Google Scholar 

  • N Wijayanti T Kietzman S Immenschuh (2005) ArticleTitleHeme oxygenase-1 gene activation by the NAD(P)H oxidase inhibitor 4-(2-aminoethyl) benzenesulfonyl fluoride via protein kinase B, p38-dependent signalling pathway in monocytes J Biol Chem 280 21820–21829 Occurrence Handle15833736 Occurrence Handle10.1074/jbc.M502943200 Occurrence Handle1:CAS:528:DC%2BD2MXksl2iu7Y%3D

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Chair of Immunology, Jagiellonian University Medical College, Kraków, Poland

    M. Kurnyta, R. Biedroń, P. Chorobik, M. Bereta & J. Marcinkiewicz

  2. Chair of Pharmacology, Jagiellonian University Medical College, Kraków, Poland

    R. Olszanecki

Authors
  1. R. Olszanecki
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  2. M. Kurnyta
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  3. R. Biedroń
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  4. P. Chorobik
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  5. M. Bereta
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  6. J. Marcinkiewicz
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Authors’ address: Janusz Marcinkiewicz, Department of Immunology, Jagiellonian University Medical College, 18 Czysta St, 31-121 Kraków, Poland

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Olszanecki, R., Kurnyta, M., Biedroń, R. et al. The role of heme oxygenase-1 in down regulation of PGE2 production by taurine chloramine and taurine bromamine in J774.2 macrophages. Amino Acids 35, 359–364 (2008). https://doi.org/10.1007/s00726-007-0609-x

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  • DOI: https://doi.org/10.1007/s00726-007-0609-x

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