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Development of a Microfluidic NMR Device for Rapid and Quantitative Detection of Tumor Markers

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Abstract

In this work, a low-field microfluidic nuclear magnetic resonance (NMR) detection device was developed by fabricating a multi-layer microfluidic NMR probe. In combination with biological sensor technology based on immunomagnetic nanoparticles (IMNPs), the microfluidic NMR detection device was used to rapidly distinguish the concentration of target tumor markers. The experimental results show that the concentration of the target tumor markers can be differentiated with high sensitivity and specificity by the rate of the transverse relaxation time change ΔT2 even with interference from other biomarkers. A good linear relationship between ΔT2 and the concentration of the target tumor markers was also found, indicating that the microfluidic NMR device could be used for quantitative detection of tumor markers. Finally, the validity of the microfluidic NMR device for detecting target tumor markers was proved by comparison with a commercial cell counter, and the results detected by the two devices have a good consistency with a correlation coefficient of 0.996. In conclusion, the presented low-field microfluidic NMR device is a potential tool for the rapid and accurate quantitative detection of tumor markers.

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Acknowledgements

We gratefully thank the financial supports from National Key Scientific Instrument and Equipment Development Project of China under Grant no. 51627808, National Natural Science Foundation of China under Grant no. 51605089, and Jiangsu Province National Natural Science Foundation of China under Grant no. BK20150609.

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Authors and Affiliations

  1. Jiangsu Key Laboratory for Design and Manufacture of Micro-Nano Biomedical Instruments, Southeast University, Nanjing, 211189, People’s Republic of China

    Rongsheng Lu, Pengkun Lei, Qing Yang, Zhonghua Ni & Hong Yi

  2. School of Mechanical Engineering, Southeast University, Nanjing, 211189, People’s Republic of China

    Rongsheng Lu, Pengkun Lei, Qing Yang, Zhonghua Ni & Hong Yi

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  1. Rongsheng Lu
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  2. Pengkun Lei
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  3. Qing Yang
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  4. Zhonghua Ni
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  5. Hong Yi
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Correspondence to Rongsheng Lu or Hong Yi.

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Lu, R., Lei, P., Yang, Q. et al. Development of a Microfluidic NMR Device for Rapid and Quantitative Detection of Tumor Markers. Appl Magn Reson 50, 357–370 (2019). https://doi.org/10.1007/s00723-018-1071-5

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  • DOI: https://doi.org/10.1007/s00723-018-1071-5