Abstract
Elaborate petals are highly diverse in morphology, structure, and epidermal differentiation and play a key role in attracting pollinators. There have been few studies on the elaborate structure of petals in the tribe Isopyreae (Ranunculaceae). Seven genera in Isopyreae (Aquilegia, Semiaquilegia, Urophysa, Isopyrum, Paraquilegia, Dichocarpum, and Leptopyrum) have petals that vary in morphology, and two genera (Enemion and Thalictrum) have no petals. The petals of nine species belonged to 7 genera in the tribe were studied to reveal their nectary structure, epidermal micromorphology and ancestral traits. The petal nectaries of Isopyreae examined in this study were located at the tip of spurs (Aquilegia yabeana and A. rockii), or the bottom of shallow sacs (Semiaquilegia adoxoides, Urophysa henryi, Isopyrum manshuricum, and Paraquilegia microphylla), a cup-shaped structure (Dichocarpum fargesii) and a bilabiate structure (Leptopyrum fumarioides). The petal nectary of eight species in Isopyreae (except A. ecalcarata) was composed of secretory epidermis, nectary parenchyma, and vascular tissues, and some sieve tubes reached the secretory parenchyma cells. Among the eight species with nectaries examined in the present study, A. yabeana had the most developed nectaries, with 10–15 layers of secretory parenchyma cells. The epidermal cells of mature petals of the nine species were divided into 11 types. Among these 11 types, there were two types of secretory cells and two types of trichomes. Aquilegia yabeana and A. rockii had the highest number of cell types (eight types), and I. manshuricum and L. fumarioides had the lowest number of cell types (three types). Aquilegia ecalcarata had no secretory cells, and the papillose conical polygonal secretory cells of D. fargesii were different from those of the other seven species with nectaries. Trichomes were found only in Aquilegia, Semiaquilegia, Urophysa, and Paraquilegia. The ancestral mode of nectar presentation in Isopyreae was petals with hidden nectar (70.58%). The different modes of nectar presentation in petals may reflect adaptations to different pollinators in Isopyreae.
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Acknowledgements
We are very grateful to Mr. Guo-Yun Zhang of the State Key Laboratory of Crop Stress Biology for Arid Areas, Northwest A&F University, for assistance with scanning electron microscopy. We are very grateful to Mr. Meng Han for collecting materials of Aquilegia and Ms. Chun-qian Ren for ancestral state reconstruction analysis.
Funding
This work was supported by the National Natural Science Foundation of China (Nos. 31770203, 31100141) and the Fundamental Research Funds for the Central Universities (No. GK202002011).
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Xiao-hui Zhang designed the research and critically revised the work. Qing-qing Zhu contributed to data analysis and wrote the manuscript. All authors read and approved the manuscript.
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Supplementary file1. Figure S1 The phylogenetic tree constructed based on complete chloroplast genomes. Numbers on branches are bootstrap supports. (JPG 1465 KB)
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Supplementary file2. Figure S2 Evolution of nectar presentation modes in Isopyreae. (JPG 363 KB)
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Zhu, QQ., Xue, C., Sun, L. et al. The diversity of elaborate petals in Isopyreae (Ranunculaceae): a special focus on nectary structure. Protoplasma 260, 437–451 (2023). https://doi.org/10.1007/s00709-022-01787-6
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DOI: https://doi.org/10.1007/s00709-022-01787-6