Abstract
Isolated Microspore Culture (IMC) is an efficient method to obtain the homozygous strain; however, it is difficult to apply in ornamental kale due to its low rate of microspore embryogenesis. Histone acetylation is an important epigenetic mechanism and may affect the changes of the microspore development pathway, promoting microspore embryogenesis. Here, microspores from three cut-flower ornamental kales, namely Crane Feather Queen (CFQ), Crane Pink (CP), and Crane Bicolor (CB), were treated with the histone deacetylase (HDAC) inhibitor suberoylanilide hydroxamic acid (SAHA) to induce embryogenesis. The haploid ‘CFQ’ microspore plantlets were doubled with colchicine. The results for ‘CFQ’ revealed that, the appropriate concentration of SAHA was 0.03 μM and obtained 17.27 embryos per bud. For ‘CP,’ the appropriate concentration of SAHA was 0.045 μM and obtained 11.19 embryos per bud. For ‘CB,’ the appropriate concentration of SAHA was 0.045 μM and obtained 6.10 embryos per bud. Haploid ‘CFQ’ microspore plantlets were treated with 75 mg/L colchicine for 7 d and the doubling rate was 41.7%. Haploid ‘CFQ’ plantlets were treated with 1000 mg/L colchicine by root-soaking for 4 h and the doubling rate was 64.3%. SAHA could promote microspore embryogenesis, and colchicine root soaking was more effective than adding colchicine to the medium for haploid plantlet doubling in cut-flower ornamental kale.
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This work was supported by grants from the National Natural Science Foundation of China (Grant No. 32002070). We would like to thank Editage (www.editage.cn) for English language editing.
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This work was supported by grants from the National Natural Science Foundation of China (Grant No. 32002070).
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J. Ren, H. Feng, C. Liu, and G. Song designed the experiments. C. Liu, G. Song, Z. Liu, J. Zou, S. Dong, and S. Du helped transplant plantlets. C. Liu performed the experiments, conducted the data analysis, and wrote the manuscript. H. Feng and B. Fang revised the manuscript. All authors reviewed and approved this submission.
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Chuanhong Liu and Gengxing Song contributed equally to this work and share first authorship.
Jie Ren and Hui Feng contributed equally to this work and share corresponding author.
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Liu, C., Song, G., Fang, B. et al. Suberoylanilide hydroxamic acid induced microspore embryogenesis and promoted plantlet regeneration in ornamental kale (Brassica oleracea var. acephala). Protoplasma 260, 117–129 (2023). https://doi.org/10.1007/s00709-022-01764-z
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DOI: https://doi.org/10.1007/s00709-022-01764-z