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Elevated CO2-induced production of nitric oxide differentially modulates nitrate assimilation and root growth of wheat seedlings in a nitrate dose-dependent manner

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Abstract

Wheat is a major staple food crop worldwide contributing approximately 20% of total protein consumed by mankind. The nitrogen and protein concentration of wheat crop and grain often decline as a result of exposure of the crop to elevated CO2 (EC). The changes in nitrogen (N) assimilation, root system architecture, and nitric oxide (NO)-mediated N signaling and expression of genes involved in N assimilation and high affinity nitrate uptake were examined in response to different nitrate levels and EC in wheat. Activity of enzyme nitrate reductase (NRA) was downregulated under EC both in leaf and root tissues. Plants grown under EC displayed enhanced production of NO and more so when nitrate supply was high. Based on exogenous supply of NO, inhibitors of NO production, and NO scavenger, regulatory role of NO on EC mediated changes in root morphology and NRA was revealed. The enhanced NO production under EC and high N levels negatively regulated the transcript abundance of NR and high affinity nitrate transporters (HATS).

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Acknowledgements

SAB acknowledges ICAR for the junior research fellowship support received during the course of the study.

Funding

ICAR-Indian Agricultural Research Institute funded (institute project-CRSCIARISIL20144047279) and provided the necessary facilities.

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  1. Sandeep B. Adavi and Lekshmy Sathee contributed equally to this work.

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  1. Division of Plant Physiology, Indian Agricultural Research Institute, New Delhi, 110012, India

    Sandeep B. Adavi & Lekshmy Sathee

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Correspondence to Lekshmy Sathee.

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Adavi, S.B., Sathee, L. Elevated CO2-induced production of nitric oxide differentially modulates nitrate assimilation and root growth of wheat seedlings in a nitrate dose-dependent manner. Protoplasma 256, 147–159 (2019). https://doi.org/10.1007/s00709-018-1285-2

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