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Effect of zinc on nectar secretion of Hibiscus rosa-sinensis L

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Abstract

Zinc toxicity in secretory cells caused a range of effects, mainly depending on metal concentration. Low concentrations activated nectary function increasing nectar secretion but secretion was greatly inhibited or stopped entirely by ongoing concentration. Water loss rate of zinc treated flower parts was significantly reduced whereas green sepals were dehydrated more rapidly in comparison to colored petals. The content of zinc, calcium, magnesium and manganese increased mainly in sepals under excess of zinc, but in the secreted nectar this metal was not evident. Morphological changes were observed in mucilage cells concerning the mucilage structure and appearance. The parenchymatic, subglandular cells displayed an early vacuolarization and cytoplasm condensation. Secretory hairs appeared to be thinner, the apical cell folded inwards and plasmolytic shrinkage became severe in all cells. The waxy cuticula showed an increased electron density. A plasmalemma detachment from the external cell walls was observed creating a gap between cell wall and plasmalemma. ER cisterns of all treated nectary hairs dominated the cytoplasm and electron dense deposits were seen within its profiles. A great number of other organelles were also present, showing electron dense deposits in their membranes as well. The vacuome was drastically reduced in all cells, except in the subglandular ones and electron dense membrane remnants were observed.

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Authors and Affiliations

  1. Department of Botany, University of Thessaloniki, 54124, Thessaloniki, Macedonia, Greece

    Thomas Sawidis, Alexandra Papadopoulou & Maria Voulgaropoulou

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  1. Thomas Sawidis
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  2. Alexandra Papadopoulou
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  3. Maria Voulgaropoulou
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Correspondence to Thomas Sawidis.

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Handling Editor: Peter Nick

Dedicated to Em. Prof. Dr. Eberhard Schnepf — teacher, colleague and friend.

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Sawidis, T., Papadopoulou, A. & Voulgaropoulou, M. Effect of zinc on nectar secretion of Hibiscus rosa-sinensis L. Protoplasma 251, 575–589 (2014). https://doi.org/10.1007/s00709-013-0557-0

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