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Comparison of two ecotypes of the metal hyperaccumulator Thlaspi caerulescens (J. & C. PRESL) at the transcriptional level

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Abstract

This paper investigates differences in gene expression among the two Thlaspi caerulescens ecotypes La Calamine (LC) and Lellingen (LE) that have been shown to differ in metal tolerance and metal uptake. LC originates from a metalliferous soil and tolerates higher metal concentrations than LE which originates from a non-metalliferous soil. The two ecotypes were treated with different levels of zinc in solution culture, and differences in gene expression were assessed through application of a cDNA microarray consisting of 1,700 root and 2,700 shoot cDNAs. Hybridisation of root and shoot cDNA from the two ecotypes revealed a total of 257 differentially expressed genes. The regulation of selected genes was verified by quantitative reverse transcriptase polymerase chain reaction. Comparison of the expression profiles of the two ecotypes suggests that LC has a higher capacity to cope with reactive oxygen species and to avoid the formation of peroxynitrite. Furthermore, increased transcripts for the genes encoding for water channel proteins could explain the higher Zn tolerance of LC compared to LE. The higher Zn tolerance of LC was reflected by a lower expression of the genes involved in disease and defence mechanisms. The results of this study provide a valuable set of data that may help to improve our understanding of the mechanisms employed by plants to tolerate toxic concentrations of metal in the soil.

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Acknowledgements

We thank Ana Assunção for her advice and for her help in providing the Thlaspi plants for our experiments. We would also like to thank the anonymous reviewers for their thoughtful comments and critiques. This work was funded by the European Community (PHYTAC, contract number QLRT-2001-00429).

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The authors declare that they have no conflict of interest.

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  1. Markus Plessl

    Present address: Julius-von-Sachs-Institut für Biowissenschaften, Universität Würzburg, 97082, Würzburg, Germany

Authors and Affiliations

  1. Institute of Biochemical Plant Pathology, Helmholtz Zentrum München—German Research Center for Environmental Health, 85764, Neuherberg, Germany

    Markus Plessl & Dieter Ernst

  2. Laboratory of Genetics, Wageningen University, 6703 BD, Wageningen, The Netherlands

    Diana Rigola & Mark G. M. Aarts

  3. Department of Biosciences, University of Kuopio, 70211, Kuopio, Finland

    Viivi H. Hassinen, Arja Tervahauta & Sirpa Kärenlampi

  4. Department of Ecology and Physiology, Faculty of Earth and Life Sciences, Vrije Universiteit Amsterdam, 1081 HV, Amsterdam, The Netherlands

    Henk Schat

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Correspondence to Dieter Ernst.

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Dedicated to Professor Cornelius Lütz on the occasion of his 65th birthday.

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Supplementary Table

Gene expression ratios of T. caerulescens clones found in the different experiments. The greatest homologues to database are indicated. Italic-typed concentration: induction or repression of a gene; italic-typed ecotype: lower or higher gene expression. Values are average ratios of three biological replicates combined with three complementary swaps. LE, Lellingen; LC, La Calamine; R, hybridisation against root cDNA; S, hybridisation against shoot cDNA; 0, 2, 10, 100, 1,000 indicates the used Zn concentrations (micromolars). Clones 1275–1281 were from A. thaliana. (XLSM 143 kb)

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Plessl, M., Rigola, D., Hassinen, V.H. et al. Comparison of two ecotypes of the metal hyperaccumulator Thlaspi caerulescens (J. & C. PRESL) at the transcriptional level. Protoplasma 239, 81–93 (2010). https://doi.org/10.1007/s00709-009-0085-0

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