Abstract
A new electroanalytical method for vitamin B1 determination, based on adsorptive chronopotentiometric stripping analysis and non-specific adsorption onto mercury film electrode, was developed and validated. Stripping chronopotentiograms showed a single well-defined oxidation wave corresponding to vitamin B1 at about − 0.43 V in citrate buffer pH 6.0. The most important experimental factors affecting the monitored electroanalytical response of vitamin B1 were investigated and optimised. Under the optimal experimental conditions, linear response of vitamin B1 was obtained in the concentration range of 5–50 mg dm−3, with the achieved limit of detection of 1.64 mg dm−3, and the limit of quantitation of 4.97 mg dm−3. A mean recovery of 97.1% and relative standard deviations of 3.75% were achieved. The developed electrochemical procedure was successfully applied for the determination of vitamin B1 in pharmaceutical products. The results of the proposed method were in good agreement with those obtained by parallel HPLC analyses, confirming an accuracy of the developed method.
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References
Combs GF Jr, McClung JP (2017) The vitamins: fundamental aspects in nutrition and health. Academic Press Inc, San Diego, p 297
Ball GFM (2005) Vitamins in foods: analysis, bioavailability and stability. CRC Press, Boca Raton, p 149
Nath A, Shope TR, Koch TR (2017) Thiamine (vitamin B1) after weight loss bariatric surgery. In: Preedy V, Rajendram R, Martin C (eds) Metabolism and pathophysiology of bariatric surgery. Elsevier Inc, San Diego, p 479
Cimpoiu C, Hosu A (2007) J Liq Chromatogr Relat Technol 30:701
Liddicoat C, Hucker B, Liang H, Vriesekoop F (2015) Food Chem 177:325
Jacobsen J (2008) Food Chem 106:1209
Markopoulou CK, Kagkadis KA, Koundourellis JE (2002) J Pharm Biomed Anal 30:1403
Lynch PLM, Young IS (2000) J Chromatogr A 881:267
Westmacott KL, Crew A, Doran O, Hart JP (2018) Talanta 181:13
Wan Q, Yang N, Ye Y (2002) Anal Sci 18:413
Oni J, Westbroek P, Nyokong T (2001) Electroanalysis 14:1165
Brahman PK, Dar RA, Pitre KS (2013) Arab J Chem 9:1889
Brahman PK, Dar RA, Pitre KS (2013) Sens Actuators B 177:807
Tyszczuk-Rotko K (2012) Food Chem 134:1239
Aboul-Kasim E (2000) J Pharm Biomed Anal 22:1047
Suturović Z, Švarc-Gajić J, Marjanović N, Kravić S (2002) Acta Period Technol 33:55
ICH (1996) Guidance for industry, Q2B validation of analytical procedures: methodology. ICH, Geneva
Miller J, Miller JC (2010) Statistics and chemometrics for analytical chemistry. Pearson Education, Harlow, p 37
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The financial support of the Ministry of Education, Science and Technological Development of the Republic of Serbia (Project III 46009) is gratefully acknowledged.
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Brezo-Borjan, T., Stojanović, Z., Suturović, Z. et al. A simple adsorptive chronopotentiometric stripping method for determination of vitamin B1 in pharmaceutical products. Monatsh Chem 151, 285–291 (2020). https://doi.org/10.1007/s00706-020-02567-9
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DOI: https://doi.org/10.1007/s00706-020-02567-9