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Design of a voltammetric immunosensor for determination of 1-nitropyrene

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Abstract

In this study a design of an immunosensor for 1-nitropyrene is illustrated. First the response of 1-nitropyrene reduced at a glassy carbon electrode between 0 and −0.8 V was investigated with respect to pH and solvent. Then polyclonal anti-1-nitropyrene antibodies isolated from a rabbit antiserum were covalently bound to the surface of the glassy carbon electrode. This modified electrode was immersed in standard solutions or sample extracts for 5–15 min. 1-Nitropyrene was bound by the antibodies, accumulated and then analyzed at pH 6.5 in another supporting electrolyte by differential pulse voltammetry. Afterwards the immunosensor could be regenerated for the next measurement by rinsing with acetonitrile–water (40:60, v/v). A linear response was found between 20 and 100 ng/cm3. The limit of detection was 10 ng/cm3 and the intraday reproducibility of three immunosensors assembled during two months was between 4.5 and 10%. This sensor was applied to the analysis of 1-nitropyrene in air particulate matter and smoked tea.

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Acknowledgments

The authors thank Professor Dr Dietmar Knopp (Technical University, Munich, Department of Hydrochemistry) for the polyclonal antiserum from rabbits and the glass filter loaded with air particulate matter.

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Authors and Affiliations

  1. Center for Analytical Chemistry, Department for Agrobiotechnology IFA Tulln, University of Natural Resources and Applied Life Sciences, Vienna, Konrad Lorenz Strasse 20, 3430, Tulln, Austria

    Norbert Stoppacher

  2. Department of Biochemistry, University of Vienna and MFPL, Dr. Bohr Gasse 9, 1030, Vienna, Austria

    Fritz Pittner

  3. Department of Analytical and Food Chemistry, University of Vienna, Waehringer Strasse 38, 1090, Vienna, Austria

    Gerhard Sontag

Authors
  1. Norbert Stoppacher
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  2. Fritz Pittner
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  3. Gerhard Sontag
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Correspondence to Gerhard Sontag.

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Stoppacher, N., Pittner, F. & Sontag, G. Design of a voltammetric immunosensor for determination of 1-nitropyrene. Monatsh Chem 140, 909–914 (2009). https://doi.org/10.1007/s00706-009-0152-4

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  • DOI: https://doi.org/10.1007/s00706-009-0152-4

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