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Complete genome sequence of a new mitovirus associated with walking iris (Trimezia northiana)

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Abstract

The complete genome sequence of a new member of the family Mitoviridae was obtained from walking iris (Trimezia northiana (Schneev.) Ravenna by high-throughput sequencing. This is the first putative mitovirus identified in a monocotyledonous plant. The new mitovirus was tentatively named “walking iris virus 1” (WIV1). The complete genome of WIV1 is 2,858 nt in length with a single ORF encoding a viral replicase (RdRp). The highest level of amino acid sequence identity was 45% to Beta vulgaris mitovirus 1. In the viral replicase, a conserved protein domain for mitovirus RNA-dependent RNA polymerase and six highly conserved motifs were detected, consistent with other members of the family Mitoviridae. Phylogenetic inferences placed WIV1 among members of the genus Duamitovirus (family Mitoviridae) in a monophyletic clade with other plant mitoviruses. Sequence comparison and phylogenetic analysis support the classification of WIV1 as a new member of the genus Duamitovirus (family Mitoviridae).

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Funding

This study was partially supported by USDA-ARS project number 8042-22000-280-00D.

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Authors and Affiliations

  1. Department of Botany and Plant Pathology, Oregon State University, Cordley Hall, 2701 SW Campus Way, Corvallis, OR, 97333, USA

    Robert Alvarez-Quinto

  2. USDA-ARS, National Germplasm Resources Laboratory, Beltsville, MD, 20705, USA

    Samuel Grinstead & Dimitre Mollov

  3. USDA-ARS, Molecular Plant Pathology Laboratory, Beltsville, MD, 20705, USA

    Samuel Grinstead

  4. Genetic Improvement for Fruits & Vegetables Laboratory, USDA-ARS, Beltsville, MD, USA

    Richard Jones

  5. USDA-ARS, Horticultural Crops Disease and Pest Management Research Unit, Corvallis, OR, 97330, USA

    Dimitre Mollov

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  1. Robert Alvarez-Quinto
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Contributions

Conceptualization: Dimitre Mollov. Formal analysis: Robert A. Alvarez-Quinto, Samuel Grinstead, Richard Jones. Investigation: Robert A. Alvarez-Quinto, Samuel Grinstead, Richard Jones, Dimitre Mollov. Methodology: Samuel Grinstead, Richard Jones, Dimitre Mollov. Supervision: Dimitre Mollov. Writing – original draft: Robert A. Alvarez-Quinto, Dimitre Mollov. Writing – review & editing: Robert A. Alvarez-Quinto, Samuel Grinstead, Richard Jones, Dimitre Mollov

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Correspondence to Dimitre Mollov.

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Communicated by Stephen John Wylie

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Alvarez-Quinto, R., Grinstead, S., Jones, R. et al. Complete genome sequence of a new mitovirus associated with walking iris (Trimezia northiana). Arch Virol 168, 273 (2023). https://doi.org/10.1007/s00705-023-05901-8

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