Abstract
Klebsiella pneumoniae causes a wide range of serious and life-threatening infections. Klebsiella phage IME183, isolated from hospital sewage, exhibited lytic activity against K. pneumoniae of capsular type K2. Transmission electron microscopy revealed that phage IME183 has a head with a diameter of 50 nm and a short tail. Its genome is 41,384 bp in length with a GC content of 52.92%. It is predicted to contain 50 open reading frames (ORFs). The results of evolutionary analysis suggest that phage IME183 should be considered a member of a new species in the genus Przondovirus.
Data availability statement
The genomic sequence obtained in the current study is available in the GenBank database under accession number MZ398245.
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Funding
This research was supported by the National Key Research and Development Program of China (no. 2018YFA0903000), the Military Biosecurity Research Program (no. 20SWAQX27), the Key Project of Beijing University of Chemical Technology (no. XK1803-06), Funds for First-class Discipline Construction (no. XK1805), and Fundamental Research Funds for Central Universities (nos. BUCTRC201917 and BUCTZY2022).
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All authors contributed to the study conception and design. Experimental work, data collection, and analysis were performed by JX and FT. This study was conceived and designed by QF, FL, and YT. The first draft of the manuscript was written by JX, and all authors commented on previous versions of manuscript. All authors read and approved the final manuscript.
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Xu, J., Tian, F., Feng, Q. et al. Genomic characterization of the novel bacteriophage IME183, infecting Klebsiella pneumoniae of capsular type K2. Arch Virol 168, 261 (2023). https://doi.org/10.1007/s00705-023-05873-9
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DOI: https://doi.org/10.1007/s00705-023-05873-9