Skip to main content
SpringerLink
Log in

Mosaic genome evolution and phylogenetics of Chrysodeixis includens nucleopolyhedrovirus (ChinNPV) and virulence of seven new isolates from the Brazilian states of Minas Gerais and Mato Grosso

  • Original Article
  • Published:
Archives of Virology Aims and scope Submit manuscript

Abstract

In a comparative analysis of genome sequences from isolates of the baculovirus Chrysodeixis includens nucleopolyhedrovirus (ChinNPV) from Brazil and Guatemala, we identified a subset of isolates possessing chimeric genomes. We identified six distinct phylogenetically incongruous regions (PIRs) dispersed in the genomes, of between 279 and 3345 bp in length. The individual PIRs possessed high sequence similarity among the affected ChinNPV isolates but varied in coverage in some instances. The donor for four of the PIRs implicated in horizontal gene transfer (HGT) was identified as Trichoplusia ni single nucleopolyhedrovirus (TnSNPV), an alphabaculovirus closely related to ChinNPV, or another unknown but closely related virus. BLAST searches of the other two PIRs returned only ChinNPV sequences, but HGT from an unknown donor baculovirus cannot be excluded. Although Chrysodeixis includens and Trichoplusia ni are frequently co-collected from soybean fields in Brazil, pathogenicity data suggest that natural coinfection of C. includens larvae with ChinNPV and TnSNPV is probably uncommon. Additionally, since the chimeric ChinNPV genomes with tracts of TnSNPV sequence were restricted to a single monophyletic lineage of closely related isolates, a model of progressive restoration of the native DNA sequence by recombination with ChinNPV possessing a fully or partially non-chimeric genome is reasonable. However, multiple independent HGT from TnSNPV to ChinNPV during the evolution of these isolates cannot be excluded. Mortality data suggest that the ChinNPV isolates with chimeric genomes are not significantly different in pathogenicity towards C. includens when compared to most other ChinNPV isolates. Exclusion of the PIRs prior to phylogenetic analysis had a large impact on the topology of part of the maximum-likelihood tree, revealing a homogenous clade of three isolates (IB, IC and ID) from Paraná state in Brazil collected in 2006, together with an isolate from Guatemala collected in 1972 (IA), comprising the lineage uniquely affected by HGT from TnSNPV. The other 10 Brazilian ChinNPV isolates from Paraná, Mato Grosso, and Minas Gerais states showed higher variability, where only three isolates from Paraná state formed a monophyletic group correlating with geographical origin.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  1. Bueno RCOF, Parra JRP, Bueno AF, Moscardi F, Oliveira JRG, Camillo MF (2007) Sem barreira. Revista Cultivar 93:12–15

    Google Scholar 

  2. Specht A, Paula-Moraes SV, Sosa-Gómez DR (2015) Host plants of Chrysodeixis includens (Walker) (Lepidoptera, Noctuidae, Plusiinae). Rev Bras Entomol 1:55–57. https://doi.org/10.1016/j.rbe.2015.09.002

    Article  Google Scholar 

  3. Moscardi F, Souza ML, Castro MEB, Moscardi ML, Szewczyk B (2011) Baculovirus pesticides: present state and future perspectives. In: Ahmad I, Ahmad F, Pichtel J (eds) Microbes and microbial technology, Springer, New York, pp 415–445. https://doi.org/10.1007/978-1-4419-7931-5_16

  4. Bernardi O, Malvestiti GS, Dourado PM, Oliveira W, Martinelli S, Berger GU, Head GP, Omoto C (2012) Assessment of the high dose concept and level of control provided by MON 87701 X MON 89788 soybean against Anticarsia gemmatalis and Pseudoplusia includens (Lepidoptera: Noctuidae) in Brazil. Pest Manag Sci 68:1083–1091. https://doi.org/10.1002/ps.3271

    Article  CAS  PubMed  Google Scholar 

  5. Jehle JA, Blissard GW, Bonning BC, Cory JS, Herniou EA, Rohrmann GF, Theilmann DA, Thiem SM, Vlak JM (2006) On the classification and nomenclature of baculoviruses: a proposal for revision. Arch Virol 151:1257–1266. https://doi.org/10.1007/s00705-006-0763-6

    Article  CAS  PubMed  Google Scholar 

  6. Harrison RL, Herniou EA, Jehle JA, Theilmann DA, Burand JP, Becnel JJ, Krell PJ, van Oers MM, Mowery JD, Bauchan GR, ICTV Report Consortium (2018) ICTV virus taxonomy profile: Baculoviridae. J Gen Virol 99:1185–1186. https://doi.org/10.1099/jgv.0.001107

    Article  CAS  PubMed  Google Scholar 

  7. Herniou EA, Arif BM, Becnel JJ, Blissard GW, Bonning B, Harrison R, Jehle JA, Theilmann DA,Vlak JM (2012) Baculoviridae. In: King AMQ, Adams MJ, Carstens EB, Lefkowitz EJ (eds) Virus taxonomy: classification and nomenclature of viruses. Ninth Report of the International Committee Taxonomy of Viruses. Elsevier Academic Press, San Diego, pp 163–173

  8. Rohrmann GF (2019) Baculovirus molecular biology, 4th edn. National Center for Biotechnology Information (US), Bethesda. https://www.ncbi.nlm.nih.gov/books/NBK543458/

  9. Herniou EA, Olszewski JA, O’Reilly DR, Cory JS (2004) Ancient coevolution of baculoviruses and their insect hosts. J Virol 78:3244–3251. https://doi.org/10.1128/jvi.78.7.3244-3251

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  10. Thézé J, Lopez-Vaamonde C, Cory JS, Herniou EA (2018) Biodiversity, evolution and ecological specialization of baculoviruses: a treasure trove for future applied research. Viruses 10:366. https://doi.org/10.3390/v10070366

    Article  CAS  PubMed Central  Google Scholar 

  11. Wang M, Hu Z (2019) Cross-talking between baculoviruses and host insects towards a successful infection. Phil Trans R Soc B 374:20180324. https://doi.org/10.1098/rstb.2018.0324

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  12. Keddie BA, Aponte GW, Volkman LE (1989) The pathway of infection of Autographa californica nuclear polyhedrosis virus in an insect host. Science 243:1728–1730. https://doi.org/10.1126/science.2648574

    Article  CAS  PubMed  Google Scholar 

  13. Ferrelli ML, Salvador R, Biedma ME, Berretta MF, Haase S, Sciocco-Cap A, Ghiringhelli P, Romanowski V (2012) Genome of Epinotia aporema granulovirus (EpapGV), a polyorganotropic fast killing betabaculovirus with a novel thymidylate kinase gene. BMC Genom 13:548. https://doi.org/10.1186/1471-2164-13-548

    Article  CAS  Google Scholar 

  14. Miele SAB, Garavaglia MJ, Belaich MN, Ghiringhelli PD (2011) Baculovirus: molecular insights on their diversity and conservation. Int J Evol Biol 2011:1–15. https://doi.org/10.4061/2011/379424

    Article  CAS  Google Scholar 

  15. Garavaglia MJ, Miele SAB, Iserte JA, Belaich MN, Ghiringhelli PD (2012) The ac53, ac78, ac101, and ac103 genes are newly discovered core genes in the family Baculoviridae. J Virol 86:12069–12079. https://doi.org/10.1128/JVI.01873-12

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  16. Javed MA, Biswas S, Willis LG, Harris S, Pritchard C, van Oers MM, Theilmann DA (2017) Autographa californica multiple nucleopolyhedrovirus AC83 is a per os infectivity factor (PIF) protein required for occlusion-derived virus (ODV) and budded virus nucleocapsid assembly as well as assembly of the PIF complex in ODV envelopes. J Virol 91:e02115-e2116. https://doi.org/10.1128/JVI.02115-16

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  17. Lange M, Wang H, Zhihong H, Jehle JA (2004) Towards a molecular identification and classification system of lepidopteran-specific baculoviruses. Virology 325:36–47. https://doi.org/10.1016/j.virol.2004.04.023

    Article  CAS  PubMed  Google Scholar 

  18. Kost TA, Condreay JP, Jarvis DL (2005) Baculovirus as versatile vectors for protein expression in insect and mammalian cells. Nat Biotechnol 23:567–575. https://doi.org/10.1038/nbt1095

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  19. Felberbaum RS (2015) The baculovirus expression vector system: a commercial manufacturing platform for viral vaccines and gene therapy vectors. Biotechnol J 10:702–714. https://doi.org/10.1002/biot.201400438

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  20. Moscardi F (1999) Assessment of the application of baculoviruses for the control of Lepidoptera. Annu Rev Entomol 44:257–289. https://doi.org/10.1146/annurev.ento.44.1.257

    Article  CAS  PubMed  Google Scholar 

  21. Popham HJR, Nusawardani T, Bonning BC (2016) Introduction to the use of baculoviruses as biological insecticides. In: Murhammer D (eds) Baculovirus and insect cell expression protocols. Methods in molecular biology, vol 1350. Humana Press, New York. https://doi.org/10.1007/978-1-4939-3043-2_19

  22. Moscardi F (2007) A nucleopolyhedrovirus for control of the velvetbean caterpillar in Brazilian soybeans. In: Vicent C, Goettel MS, Lazarovits G (eds) Biological control: a global perspective, vol 38, pp 344–352. https://doi.org/10.1079/9781845932657.0344.

  23. Haase S, Sciocco-Cap A, Romanowski V (2015) Baculovirus insecticides in Latin America: historical overview, current status and future perspectives. Viruses 7:2230–2267. https://doi.org/10.3390/v7052230

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  24. Sosa-Gómez DR, Morgado FS, Corrêa RFT, Silva LA, Ardisson-Araújo DMP, Rodrigues BMP, Oliveira EE, Aguiar RWS, Ribeiro BM (2020) Entomopathogenic viruses in the neotropics: current status and recently discovered species. NeotropEntomol 49:315–331. https://doi.org/10.1007/s13744-020-00770-1

    Article  Google Scholar 

  25. Craveiro SR, Inglis PW, Togawa RC, Grynberg P, Melo FL, Ribeiro ZMA, Ribeiro BM, Báo SN, Castro MEB (2015) The genome sequence of Pseudoplusia includens single nucleopolyhedrovirus and an analysis of p26 gene evolution in the baculoviruses. BMC Genom 16:127. https://doi.org/10.1186/s12864-015-1323-9

    Article  Google Scholar 

  26. Craveiro SR, Santos LAVM, Togawa RC, Inglis PW, Grynberg P, Ribeiro ZMA, Ribeiro BM, Castro MEB (2016) Complete genome sequences of six Chrysodeixis includens nucleopolyhedrovirus isolates from Brazil and Guatemala. Microbiol Resour Ann 4:e01192-e1216. https://doi.org/10.1128/genomeA.01192-16

    Article  Google Scholar 

  27. Li L, Li Q, Willis LG, Erlandson M, Theilmann DA, Donly C (2005) Complete comparative genomic analysis of two field isolates of Mamestra configurata nucleopolyhedrovirus-A. J Gen Virol 86:91–105. https://doi.org/10.1099/vir.0.80488-0

    Article  CAS  PubMed  Google Scholar 

  28. Brito AF, Braconi CT, Weidmann M, Dilcher M, Alves JM, Gruber A, Zanotto PM (2015) The pangenome of the Anticarsia gemmatalis multiple nucleopolyhedrovirus (AgMNPV). Genome Biol Evol 8:94–108. https://doi.org/10.1093/gbe/evv231

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  29. Brito AF, Melo FL, Ardisson-Araújo DMP, Sihler W, Souza ML, Ribeiro BM (2018) Genome-wide diversity in temporal and regional populations of the betabaculovirus Erinnyis ello granulovirus (ErelGV). BMC Genomics 19:698. https://doi.org/10.1186/s12864-018-5070-6

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  30. Alexandre TM, Ribeiro ZM, Craveiro SR, Cunha F, Fonseca IC, Moscardi F, Castro MEB (2010) Evaluation of seven viral isolates as potential biocontrol agents against Pseudoplusia includens (Lepidoptera: Noctuidae) caterpillars. J Invertebr Pathol 105:98–104. https://doi.org/10.1016/j.jip.2010.05.015

    Article  PubMed  Google Scholar 

  31. Craveiro SR, Melo FL, Ribeiro ZM, Ribeiro BM, Bao SN, Inglis PW, Castro MEB (2013) Pseudoplusia includens single nucleopolyhedrovirus: genetic diversity, phylogeny and hypervariability of the pif-2 gene. J Invertebr Pathol 114:258–267. https://doi.org/10.1016/j.jip.2013.08.005

    Article  CAS  PubMed  Google Scholar 

  32. Craveiro SR, Inglis PW, Monteiro LLS, Santos LAVM, Togawa RC, Ribeiro ZMA, Ribeiro BM, Castro MEB (2020) Complete genome sequences of seven new Chrysodeixis includens nucleopolyhedrovirus isolates from Minas Gerais and Mato Grosso states in Brazil. Microbiol Resour Announc 9:e01501-e1519. https://doi.org/10.1128/MRA.01501-19

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  33. Aguirre E, Beperet I, Williams T, Caballero P (2019) Genetic variability of Chrysodeixis includens nucleopolyhedrovirus (ChinNPV) and the insecticidal characteristics of selected genotypic variants. Viruses 11:581. https://doi.org/10.3390/v11070581

    Article  CAS  PubMed Central  Google Scholar 

  34. Xu X, Liu H (2008) Baculovirus surface display of E2 envelope glycoprotein of classical swine fever virus and immunogenicity of the displayed proteins in a mouse model. Vaccine 26:5455–5460. https://doi.org/10.1016/j.vaccine.2008.07.090

    Article  CAS  PubMed  Google Scholar 

  35. Morgado FS, Silva LA, Bernardes LM, Czepak C, Strand MR, Ribeiro BM (2020) Trichoplusia ni and Chrysodeixis includens larvae show different susceptibility to Chrysodeixis includens single nucleopolyhedrovirus per os infection. J Pest Sci 93:1019–1029. https://doi.org/10.1007/s10340-020-01217-7

    Article  Google Scholar 

  36. Harrison RL, Rowley DL, Popham HJR (2019) A novel alphabaculovirus from the soybean looper, Chrysodeixis includens, that produces tetrahedral occlusion bodies and encodes two copies of he65. Viruses 11:579. https://doi.org/10.3390/v11070579

    Article  CAS  PubMed Central  Google Scholar 

  37. Blissard GW, Rohrmann GF (1990) Baculovirus diversity and molecular biology. Ann Rev Entomol 35:127–155. https://doi.org/10.1146/annurev.en.35.010190.001015

    Article  CAS  Google Scholar 

  38. O’Reilly DR, Miller LK (1990) Regulation of expression of a baculovirus ecdysteroid UDP-glucosyltransferase gene. J Virol 64:1321–1328. https://doi.org/10.1128/JVI.64.3.1321-1328.1990

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  39. Kamita GK, Maeda S, Hammock BD (2003) High-frequency homologous recombination between baculoviruses involves DNA replication. J Virol 77:13053–13061. https://doi.org/10.1128/JVI.77.24.13053-13061.2003

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  40. Erlandson MA (2009) Genetic variation in field populations of baculoviruses: mechanisms for generating variation and its potential role in baculovirus epizootiology. Virol Sin 24:458–469. https://doi.org/10.1007/s12250-009-3052-1

    Article  Google Scholar 

  41. Gilbert C, Peccoud J, Chateigner A, Moumen B, Cordaux R, Herniou EA (2016) Continuous influx of genetic material from host to virus populations. PLoS Genet 12(2):e1005838. https://doi.org/10.1371/journal.pgen.1005838

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  42. Hughes AL, Friedman R (2003) Genome-wide survey for genes horizontally transferred from cellular organisms to baculoviruses. Mol Biol Evol 20:979–987. https://doi.org/10.1093/molbev/msg107

    Article  CAS  PubMed  Google Scholar 

  43. Ikeda M, Hamajima R, Kobayashi M (2015) Baculoviruses: diversity, evolution and manipulation of insects. Entomol Sci 18:1–20. https://doi.org/10.1111/ens.12105

    Article  Google Scholar 

  44. Hill T, Unckless RL (2017) Baculovirus molecular evolution via gene turnover and recurrent positive selection of key genes. J Virol 91:e01319-e1417. https://doi.org/10.1128/JVI.01319-17

    Article  PubMed  PubMed Central  Google Scholar 

  45. Rohrmann GF (2013) Baculovirus molecular biology, 3rd edn. National Center for Biotechnology Information (US), Bethesda. https://www.ncbi.nlm.nih.gov/books/NBK114593/

  46. Muñoz D, Murillo R, Krell PJ, Vlak JM, Caballero P (1999) Four genotypic variants of a Spodoptera exigua nucleopolyhedrovirus (Se-SP2) are distinguishable by a hypervariable genomic region. Virus Res 59:61–74. https://doi.org/10.1016/S0168-702(98)00125-7

    Article  PubMed  Google Scholar 

  47. Cory JS, Green BM, Paul RK, Hunter-Fujita F (2005) Genotypic and phenotypic diversity of a baculovirus population within an individual insect host. J Invertebr Pathol 89:101–111. https://doi.org/10.1016/j.jip.2005.03.008

    Article  PubMed  Google Scholar 

  48. Greene GL, Leppla NC, Dicherson WA (1976) Velvetbean caterpillar: a rearing produced and artificial medium. J Econ Entomol 4:487–488. https://doi.org/10.1093/jee/69.4.487

    Article  Google Scholar 

  49. Morales L, Moscardi F (1993) Comparação entre duas metodologias de bioensaios para vírus entomopatogênicos. An Soc Entomol Bras 22:535–540

    Google Scholar 

  50. Finney DJ (1971) Probit analysis, 3rd edn. Cambridge University Press, Cambridge. https://doi.org/10.1002/jps.2600600940

  51. Busvine JR (1971) A critical review of the techniques for testing insecticides. Commonwealth Agricultural Bureaux, London, pp 263–288 (345 pp)

  52. Morales L, Moscardi F, Dr S-G, Paro F, Soldório I (2001) Fluorescent brighteners improve Anticarsia gemmatalis (Lepidoptera: Noctuidae) nucleopolyhedrovirus (AgMNPV) activity on AgMNPV-suscetible and resistant strains of the insect. Biol Control 20:247–253. https://doi.org/10.1006/bcon.2000.0904

    Article  CAS  Google Scholar 

  53. Darling AC, Mau B, Blattner FR, Perna NT (2004) Mauve: multiple alignment of conserved genomic sequence with rearrangements. Genome Res 14:1394–1403. https://doi.org/10.1101/gr.2289704

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  54. Katoh K, Standley DM (2013) MAFFT Multiple Sequence Alignment Software Version 7: Improvements in performance and usability. Mol Biol Evol 30:772–780. https://doi.org/10.1093/molbev/mst010

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  55. Hillary W, Lin SH, Upton C (2011) Base-By-Base version 2: single nucleotide-level analysis of whole viral genome alignments. BMC Microb Inform Exp 1:2. https://doi.org/10.1186/2042-5783-1-2

    Article  CAS  Google Scholar 

  56. Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ (1990) Basic local alignment search tool. J Mol Biol 215:403–410. https://doi.org/10.1016/S0022-2836(05)80360-2

    Article  CAS  PubMed  Google Scholar 

  57. Nguyen L-T, Schmidt HA, von Haeseler A, Minh BQ (2015) IQ-TREE: a fast and effective stochastic algorithm for estimating maximum likelihood phylogenies. Mol Biol Evol 32:268–274. https://doi.org/10.1093/molbev/msu300

    Article  CAS  PubMed  Google Scholar 

  58. Bruen T, Philippe H, Bryant D (2006) A simple and robust statistical test for detecting the presence of recombination. Genetics 172:2665–2681. https://doi.org/10.1534/genetics.105.048975

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  59. Martin DP, Murrell B, Golden M, Khoosal A, Muhire B (2015) RDP4: detection and analysis of recombination patterns in virus genomes. Virus Evol. https://doi.org/10.1093/ve/vev003

    Article  PubMed  PubMed Central  Google Scholar 

  60. Castresana J (2000) Selection of conserved blocks from multiple alignments for their use in phylogenetic analysis. Mol Biol Evol 17:540–552

    Article  CAS  PubMed  Google Scholar 

  61. Le T, Hoa Wu, Tieqiao Robertson A, Bulach D, Cowan P, Goodge K, Tribe D (1997) Genetically variable triplet repeats in a RING-finger ORF of Helicoverpa species baculoviruses. Virus Res 49:67–77. https://doi.org/10.1016/S0168-1702(97)01454-8

    Article  CAS  PubMed  Google Scholar 

  62. Harrison RL, Lynn DE (2007) Genomic sequence analysis of a nucleopolyhedrovirus isolated from the diamondback moth, Plutella xylostella. Virus Genes 35:857–873. https://doi.org/10.1007/s11262-007-0136-6

    Article  CAS  PubMed  Google Scholar 

  63. Zhou J-B, Li X-Q, De-Eknamkul W, Suraporn S, Xu J-P (2012) Identification of a new Bombyx mori nucleopolyhedrovirus and analysis of its bro gene family. Virus Genes 44:539–547. https://doi.org/10.1007/s11262-012-0721-1

    Article  CAS  PubMed  Google Scholar 

  64. Santos ER, Oliveira LB, Peterson L, Sosa-Gómez DR, Ribeiro BM, Ardisson-Araújo DMP (2018) The complete genome sequence of the first hesperiid-infecting alphabaculovirus isolated from the leguminous pest Urbanus proteus (Lepidoptera: Hesperiidae). Virus Res 249:76–84. https://doi.org/10.1016/j.virusres.2018.03.009

    Article  CAS  PubMed  Google Scholar 

  65. Kang W, Suzuli M, Evgueni Z, Okano K, Maeda S (1999) Characterization of baculovirus repeated open reading frames (bro) in Bombyx mori nucleopolyhedrovirus. J Virol 73:10339–10345. https://doi.org/10.1128/JVI.73.12.10339-10345.1999

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  66. Bideshi DK, Renault S, Stasiak K, Federici BA, Bigot Y (2003) Phylogenetic analysis and possible function of bro-like genes, a multigene family widespread among large double-stranded DNA viruses of invertebrates and bacteria. J Gen Virol 84:2531–2544. https://doi.org/10.1099/vir.0.19256-0

    Article  CAS  PubMed  Google Scholar 

  67. Williams T, Virto C, Murillo R, Caballero P (2017) Covert infection of insects by baculoviruses. Front Microbiol 8:1337. https://doi.org/10.3389/fmicb.2017.01337

    Article  PubMed  PubMed Central  Google Scholar 

  68. Cory JS, Myers JH (2003) The ecology and evolution of baculoviruses. Annu Rev Ecol Evol S 34:239–272. https://doi.org/10.1146/annurev.ecolsys.34.011802.132402

    Article  Google Scholar 

  69. Del Rincón-Castro M, Ibarra J (1997) Genotypic divergence of three single nuclear polyhedrosis virus (SNPV) strains from the cabbage looper, Trichoplusia ni. Biochem Syst Ecol 25:287–295. https://doi.org/10.1016/S0305-1978(97)00002-1

    Article  Google Scholar 

  70. Gilbert C, Chateigner A, Ernenwein L, Barbe V, Bezier A, Herniou EA, Cordaux R (2014) Population genomics supports baculoviruses as vectors of horizontal transfer of insect transposons. Nat Commun 5:3348. https://doi.org/10.1038/ncomms4348

    Article  CAS  PubMed  Google Scholar 

  71. Herniou EA, Luque T, Chen X, Vlak JM, Winstanley D, Cory JS, O’Reilly DR (2001) Use of whole genome sequence data to infer baculovirus phylogeny. J Virol 75:8117–8126. https://doi.org/10.1128/JVI.75.17.8117-8126.2001

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  72. Doolittle WF (1999) Phylogenetic classification and the universal tree. Science 284:2124–2129. https://doi.org/10.1126/science.284.5423.2124

    Article  CAS  PubMed  Google Scholar 

  73. Ardisson-Araújo DMP, de Melo FL, Andrade MS et al (2014) Genome sequence of Erinnyis ello granulovirus (ErelGV), a natural cassava hornworm pesticide and the first sequenced sphingid-infecting betabaculovirus. BMC Genom 15:856. https://doi.org/10.1186/1471-2164-15-856

    Article  CAS  Google Scholar 

  74. Trentin LB, Santos ER, Junior AGO, Sosa-Gómez DR, Ribeiro BM, Ardisson-Araújo DMP (2019) The complete genome of Rachiplusia nu nucleopolyhedrovirus (RanuNPV) and the identification of a baculoviral CPD-photolyase homolog. Virology 534:64–71. https://doi.org/10.1016/j.virol.2019.05.019

    Article  CAS  PubMed  Google Scholar 

  75. Sugiura N, Setoyama Y, Chiba M, Kimata K, Watanabe H (2011) Baculovirus envelope protein ODV-E66 is a novel chondroitinase with distinct substrate specificity. J Biol Chem 286:29026–29034. https://doi.org/10.1074/jbc.M111.251157

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  76. Kawaguchi Y, Sugiura N, Onishi M, Kimata K, Kimura M, Kakuta Y (2012) Crystallization and X-ray diffraction analysis of chondroitin lyase from baculovirus: envelope protein ODV-E66. Acta Crystallogr Sect F Struct Biol Cryst Commun 68:190–192. https://doi.org/10.1107/S1744309111053164

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  77. Croizier G, Ribeiro HCT (1992) Recombination as a possible major cause of genetic heterogeneity in Anticarsia gemmatalis nuclear polyhedrosis virus populations. Virus Res 26:183–196. https://doi.org/10.1016/0168-1702(92)90012-X

    Article  CAS  Google Scholar 

  78. Kondo A, Maeda S (1991) Host range expansion by recombination of the baculoviruses Bombyx mori nuclear polyhedrosis virus and Autographa californica nuclear polyhedrosis virus. J Virol 65:3625–3632. https://doi.org/10.1128/JVI.65.7.3625-3632.1991

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  79. Wu X, Cao C, Xu Y, Lu X (2004) Construction of a host range-expanded hybrid baculovirus of BmNPV and AcNPV, and knockout of cysteinase gene for more efficient expression. Sci China Ser C Life Sci 47:406–415. https://doi.org/10.1007/BF03187098

    Article  CAS  Google Scholar 

Download references

Acknowledgements

This work was supported in part from Fundação de Apoio a Pesquisa do Distrito Federal (FAPDF) grant number 193.001532/2016 to BMR/Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and grant number 305756/2017-6 to BMR/Universidade de Brasília (UnB)/Embrapa Recursos Genéticos e Biotecnologia. Further funding was provided by Embrapa and grant 193.001531/2016-PRONEX (Programa de Apoio a Núcleos de Excelência). The authors also wish to thank Zilda M.A. Ribeiro for laboratory support.

Author information

Authors and Affiliations

  1. Embrapa Recursos Genéticos e Biotecnologia, Parque Estação Biológica, Brasília, DF, Brazil

    Peter W. Inglis, Luis Arthur V. M. Santos, Saluana R. Craveiro & Maria Elita B. Castro

  2. Departamento de Biologia Celular, Universidade de Brasília-UnB, Brasília, DF, Brazil

    Bergmann M. Ribeiro

Authors
  1. Peter W. Inglis
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Luis Arthur V. M. Santos
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Saluana R. Craveiro
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Bergmann M. Ribeiro
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Maria Elita B. Castro
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

MEBC contributed to the study conception, funding acquisition and design. BMR also contributed to funding acquisition. Material preparation, data collection and analysis were performed by LAVMS, SRC and PWI. The first draft of the manuscript was written by PWI, MEBC and BMR and all authors read and approved the final manuscript.

Corresponding author

Correspondence to Peter W. Inglis.

Ethics declarations

Conflict of interest

The authors declare that they have no conflict of interest.

Additional information

Handling Editor: T. K. Frey.

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Electronic supplementary material

Below is the link to the electronic supplementary material.

705_2020_4858_MOESM1_ESM.tif

Supplementary file1 Fig. S1 Mauve collinearity plot of ChinNPV genomes along with TnSNPV and ChchSNPV genomes (TIF 1905 kb)

705_2020_4858_MOESM2_ESM.eps

Supplementary file2 Fig. S2 Midpoint-rooted ML tree of ChinNPV, TnSNPV and ChchSNPV genome sequences. The tree was calculated in IQTREE using an unpartitioned TVM+F+R5 model, selected as optimal according to BIC. Support values given above branches are ultrafast bootstrap percentages. GenBank numbers of genome sequences are given in Tables 3 and 4 (EPS 1239 kb)

705_2020_4858_MOESM3_ESM.eps

Supplementary file3 Fig. S3 Midpoint-rooted ML tree of ChinNPV, TnSNPV and ChchSNPV genome sequences, with phylogenetically incongruent regions identified among ChinNPV isolates deleted. The tree was calculated in IQTREE using an unpartitioned TVM+F+R6 model, selected as optimal according to BIC. Support values given above branches are ultrafast bootstrap percentages. GenBank numbers of genome sequences are given in Tables 3 and 4 (EPS 1152 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Inglis, P.W., Santos, L.A.V.M., Craveiro, S.R. et al. Mosaic genome evolution and phylogenetics of Chrysodeixis includens nucleopolyhedrovirus (ChinNPV) and virulence of seven new isolates from the Brazilian states of Minas Gerais and Mato Grosso. Arch Virol 166, 125–138 (2021). https://doi.org/10.1007/s00705-020-04858-2

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00705-020-04858-2

Search

Navigation