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Genomic sequencing and characterization of a novel group of canine bufaviruses from Henan province, China

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Abstract

Canine bufavirus (CBuV) is a novel protoparvovirus of dogs that was first reported in 2018 in Italy. The prevalence and genetic diversity of CBuV in China are not clear. In this study, a total of 115 canine fecal samples were collected from northern China in 2019, and two of the samples tested positive for CBuV DNA by PCR. These two field CBuV strains were designated Henan38 and Henan44. The complete genomic sequences of Henan38 and Henan44 were obtained by gap-filling PCR, sequenced, and assembled. Phylogenetic analysis demonstrated that the two strains clustered together in a novel group that was distant from previously reported CBuV strains. This study will strengthen our understanding of the epidemiology and genetic diversity of CBuV in China.

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Funding

This work was supported by the National Natural Science Foundation of China (31702271) and the Guangdong Provincial Natural Science Foundation (2017A030310367).

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Authors and Affiliations

  1. College of Veterinary Medicine, South China Agricultural University, Guangzhou, 510642, Guangdong Province, People’s Republic of China

    Ran Shao, Feiyan Zheng, Siqi Cai, Jinzhao Ji, Zixin Ren, Jiawei Zhao, Liyan Wu, Jiajun Ou, Gang Lu & Shoujun Li

  2. Guangdong Provincial Key Laboratory of Prevention and Control for Severe Clinical Animal Diseases, Guangzhou, 510642, Guangdong Province, People’s Republic of China

    Ran Shao, Feiyan Zheng, Siqi Cai, Jinzhao Ji, Zixin Ren, Jiawei Zhao, Liyan Wu, Jiajun Ou, Gang Lu & Shoujun Li

  3. Guangdong Technological Engineering Research Center for Pet, Guangzhou, 510642, Guangdong Province, People’s Republic of China

    Ran Shao, Feiyan Zheng, Siqi Cai, Jinzhao Ji, Zixin Ren, Jiawei Zhao, Liyan Wu, Jiajun Ou, Gang Lu & Shoujun Li

Authors
  1. Ran Shao
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  2. Feiyan Zheng
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  3. Siqi Cai
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  4. Jinzhao Ji
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  5. Zixin Ren
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  6. Jiawei Zhao
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  7. Liyan Wu
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  8. Jiajun Ou
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  9. Gang Lu
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  10. Shoujun Li
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Corresponding authors

Correspondence to Gang Lu or Shoujun Li.

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Conflict of interest

The authors declare that they do not have a conflict of interest.

Ethical approval

This research did not involve any human participants or experiments on live animals.

Accession numbers

The complete genome sequences of Henan38 and Henan44 were deposited in the NCBI nucleotide database under the accession numbers MT364251 and MT364252, respectively.

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Handling Editor: Ana Cristina Bratanich

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Shao, R., Zheng, F., Cai, S. et al. Genomic sequencing and characterization of a novel group of canine bufaviruses from Henan province, China. Arch Virol 165, 2699–2702 (2020). https://doi.org/10.1007/s00705-020-04785-2

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  • DOI: https://doi.org/10.1007/s00705-020-04785-2

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